Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa

Nasiri, Aliakbar; Gharebagh, Roghayeh Afsar; Nojoumi, Seyed Ali; Akbarizadeh, Majid Reza; Harirchi, Sharareh; Arefnezhad, Masoud; Sahraei, Shahla; Hesaraki, Mehran; Afshari, Mahdi; Javadian, Fereshteh; Asadi, Maryam Sheykhzade; Shahi, Zahra; Sargazi, Aliyeh

doi:10.15171/ijbsm.2016.06

Cited by 12 publications

(7 citation statements)

References 12 publications

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“…Our results showed that AgNPs had good antibacterial activity at concentration of 15.6 μg/ml. Similar to our study, many studies have demonstrated the antibacterial effect of AgNPs on Gram-positive and Gram-negative bacteria (38)(39)(40). Although AgNPs had good antibacterial effects, they may also cause significant cytotoxicity (33).…”

Section: Discussionsupporting

confidence: 87%

Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa

et al. 2017

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Background and Objectives: Nowadays, the prevalence of multidrug-resistant pathogens such as Pseudomonas aeruginosa is increasing worldwide. Many studies have been seeking new treatment strategies to treat infections caused by these microorganisms. Silver nanoparticles (AgNPs) along with L-arginine have significant antimicrobial effects and could be used as alternatives for ineffective drugs.Methods: In this study, the antibacterial activity of AgNPs, L-arginine and various concentrations of AgNPs along with L-arginine (12.5 and 25 mg/ml) were investigated against P. aeruginosa PAO1 using the broth macrodilution method.Results: Minimum inhibitory concentration of AgNPs, L-arginine and AgNPs combined with 25 and 12.5 mg/ml L-arginine was 15.6 μg/ml, 25 mg/ml, 1.9 μg/ml and 3.9 μg/ml, respectively. Minimum bactericidal concentration of AgNPs, L-arginine and AgNPs combined with 25 and 12.5 mg/ml L-arginine was 31.2 μg/ml, 50 mg/ml, 3.9 μg/ml and 7.8 μg/ml, respectively.Conclusion: Our study suggests that AgNPs along with L-arginine can be used as an alternative antibacterial agent against P. aeruginosa, and might be useful for treatment of wound infections.

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Section: Discussionsupporting

confidence: 87%

Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa

et al. 2017

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“…This difference may be due to research on a specific clinical specimen (Urine only). In 2016, Nasiri et al 26 reported 1 mg/ml MIC of AgNPs against P. aeruginosa isolated from urine cultures of hospitalized patients. Their MIC values were slightly lower than the ones in the present study.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Silver Nanoparticles on Pyocyanin Production of Pseudomonas aeruginosa Isolated From Clinical Specimens

Najafi¹,

Moghaddam²,

Yousefi³

2021

AJMB

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Background: Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen causing a wide range of human infections. The organism is resistant to a wide range of antibiotics. The purpose of this study was to investigate the effect of AgNPs on pyocyanin pigment production of P. aeruginosa bacteria isolated from clinical specimens. Methods: In this study, 15 clinical isolates of P. aeruginosa were collected from different specimens of hospitalized patients. P. aeruginosa was detected by biochemical and molecular (detection of pbo1 gene by colony PCR method) methods and the MIC and MBC of AgNPs were determined by agar dilution method. Inhibition of P. aeruginosa pyocyanin production at AgNPs concentrations of 0, 0.3, 0.5, 1 and 1.5 mg/ml of was studied with OD of 520 nm. Results: The mean MIC and MBC of AgNPs were 1.229 and 1.687 mg/ml, respectively. Pyocyanin production was investigated for all isolates at different concentrations of nanoparticles, and their comparison showed that with increasing nanoparticle concentration, pyocyanin production significantly decreased (p<0.05). Conclusion: According to the results of this study, AgNPs had an inhibitory effect on P. aeruginosa and its pigment production and with increasing nanoparticles concentration, pigment production decreased; therefore, it seems that the nanoparticles can be used to treat and prevent diseases caused by P. aeruginosa.

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“…Significant research on silver NPs as antimicrobial agents has been reported in the literature and much research has also been done on the efficacy of silver NPs against P. aeruginosa [88][89][90][91][92][93][94][95][96][97][98][99]. Due to their undisputable antibacterial properties, silver NPs are among the most commonly exploited nanomaterials in commercialized products [100].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

Armijo¹,

Wawrzyniec²,

Kopciuch³

et al. 2020

J Nanobiotechnol

115

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Background: Novel methods are necessary to reduce morbidity and mortality of patients suffering from infections with Pseudomonas aeruginosa. Being the most common infectious species of the Pseudomonas genus, P. aeruginosa is the primary Gram-negative etiology responsible for nosocomial infections. Due to the ubiquity and high adaptability of this species, an effective universal treatment method for P. aeruginosa infection still eludes investigators, despite the extensive research in this area. Results:We report bacterial inhibition by iron-oxide (nominally magnetite) nanoparticles (NPs) alone, having a mean hydrodynamic diameter of ~ 16 nm, as well as alginate-capped iron-oxide NPs. Alginate capping increased the average hydrodynamic diameter to ~ 230 nm. We also investigated alginate-capped iron-oxide NP-drug conjugates, with a practically unchanged hydrodynamic diameter of ~ 232 nm. Susceptibility and minimum inhibitory concentration (MIC) of the NPs, NP-tobramycin conjugates, and tobramycin alone were determined in the PAO1 bacterial colonies. Investigations into susceptibility using the disk diffusion method were done after 3 days of biofilm growth and after 60 days of growth. MIC of all compounds of interest was determined after 60-days of growth, to ensure thorough establishment of biofilm colonies. Conclusions:Positive inhibition is reported for uncapped and alginate-capped iron-oxide NPs, and the corresponding MICs are presented. We report zero susceptibility to iron-oxide NPs capped with polyethylene glycol, suggesting that the capping agent plays a major role in enabling bactericidal ability in of the nanocomposite. Our findings suggest that the alginate-coated nanocomposites investigated in this study have the potential to overcome the bacterial biofilm barrier. Magnetic field application increases the action, likely via enhanced diffusion of the iron-oxide NPs and NP-drug conjugates through mucin and alginate barriers, which are characteristic of cystic-fibrosis respiratory infections. We demonstrate that iron-oxide NPs coated with alginate, as well as alginate-coated magnetite-tobramycin conjugates inhibit P. aeruginosa growth and biofilm formation in established colonies. We have also determined that susceptibility to tobramycin decreases for longer culture times. However, susceptibility to the iron-oxide NP © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article'

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Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa

Cited by 12 publications

References 12 publications

Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa

Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa

The Effect of Silver Nanoparticles on Pyocyanin Production of Pseudomonas aeruginosa Isolated From Clinical Specimens

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

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