The Effect of Silver Nanoparticles on Pyocyanin Production of Pseudomonas aeruginosa Isolated From Clinical Specimens

Abstract

Background: Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen causing a wide range of human infections. The organism is resistant to a wide range of antibiotics. The purpose of this study was to investigate the effect of AgNPs on pyocyanin pigment production of P. aeruginosa bacteria isolated from clinical specimens.
 Methods: In this study, 15 clinical isolates of P. aeruginosa were collected from different specimens of hospitalized patients. P. aeruginosa was detected by biochemical and molecular (detection of pbo1 gene by colony PCR method) methods and the MIC and MBC of AgNPs were determined by agar dilution method. Inhibition of P. aeruginosa pyocyanin production at AgNPs concentrations of 0, 0.3, 0.5, 1 and 1.5 mg/ml of was studied with OD of 520 nm.
 Results: The mean MIC and MBC of AgNPs were 1.229 and 1.687 mg/ml, respectively. Pyocyanin production was investigated for all isolates at different concentrations of nanoparticles, and their comparison showed that with increasing nanoparticle concentration, pyocyanin production significantly decreased (p<0.05).
 Conclusion: According to the results of this study, AgNPs had an inhibitory effect on P. aeruginosa and its pigment production and with increasing nanoparticles concentration, pigment production decreased; therefore, it seems that the nanoparticles can be used to treat and prevent diseases caused by P. aeruginosa.

Delpinium uncinatum mediated green synthesis of AgNPs and its antioxidant, enzyme inhibitory, cytotoxic and antimicrobial potentials

“…Small round to fibrous type cells verified the impact of inhibition (Fig 9 ). A similar study of the Hep-G2 cell viability was reported previously [61]. The cytotoxicity of AgNPs is a direct result of oxidative stress induced by Ag ion release from AgNPs.…”

The influence of nanomaterials on pyocyanin production by Pseudomonas aeruginosa

“…Pseudomonas aeruginosa is an opportunistic pathogen belonging to the ESKAPE group, often associated with nosocomial infections and complications in cystic fibrosis patients (Hall et al 2016;Grygorcewicz et al 2020). Therefore, many researchers focus on eliminating P. aeruginosa employing different nanomaterials, mainly metal oxides (Lee et al 2014;Zanni et al 2017;Saleh et al 2019;El-Deeb et al 2020;Ali et al 2020;Najafi et al 2021) However, it was reported that Pseudomonas spp. biofilm formation or cell respiration can be stimulated by nanomaterials, such as silver nanoparticles, zinc oxide, and hybrid nanomaterial consisting of silica nanotubes modified with titanium dioxide (Yang and Alvarez 2015;Augustyniak et al 2020;Ouyang et al 2020).…”

Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cells

“…After incubation, the first well in which no growth was observed was considered as MIC. From this dilution and higher dilutions, 10 μL were taken on nutrient agar culture medium, the dilution in which no growth was observed was considered as MBC [34].…”