Evaluation of the antimicrobial activity of cationic polyethylenimines on dry surfaces

Abstract

was evaluated when the polymer was applied as a coating to various surfaces using a paint-like procedure. Antimicrobial activity of PEIþ as a function of time was determined using an assay for long-term survival involving placement of single drops of various bacterial concentrations on dry surfaces. These data were compared with an assay method where bacteria were applied by spraying and surfaces were incubated overnight under agar. PEIþ-coated surfaces were found to be highly bactericidal after 30 min when bacteria were sprayed onto surfaces. However, when bacteria were applied as single drops, PEIþ-coated surfaces were less biocidal at short contact times particularly for A. baumannii and E. coli. The observations are explained in the context of the difference in drying time between drops deposited on uncoated surfaces and PEIþ-coated surfaces and the sensitivity of bacterial survival to dehydration. These results demonstrate that PEIþ-coated surfaces are not effectively biocidal for some types of bacteria under certain conditions and that the method of assaying bactericidal efficiency can greatly affect the results obtained.

Antifouling and antimicrobial biomaterials: an overview

“…Besides S. aureus and E. coli , Acinetobacter baumannii has also been used to demonstrate the antimicrobial activity of cationic N , N ‐dodecyl,methyl‐PEI. Specifically, hydrophobic PEI‐coated surfaces were found to be highly bactericidal after 30 min when bacteria were sprayed onto dry surfaces, but less bactericidal when strains were applied as single drops .…”

Polymers for Combating Biocorrosion

“…To covalently graft antibacterial polymers, it is necessary to immobilize a uniform monolayer of initiators on the metal substrate surfaces, as shown in Figure 3. The immobilization of initiators on the metallic substrate surfaces is commonly achieved by a direct coupling of chloromethyl-/chlorosulfonylterminated silane (Yuan et al, 2009a(Yuan et al, ,b,c, 2010aYang et al, 2014) and bromomethyl-terminated biomimetic catechol (Koplin et al, 2008). Monomers containing tertiary amino groups, such as 2-dimethylaminoethyl methacrylate (DMAEMA) and 4-vinyl pyridine (4VP), have been extensively used in synthesizing antimicrobial polymers via surface graft polymerization (Figure 3) (Fan et al, 2005;Koplin et al, 2008;Yuan et al, 2009aYuan et al, ,b,c, 2010aYang et al, 2014).…”

“…The immobilization of initiators on the metallic substrate surfaces is commonly achieved by a direct coupling of chloromethyl-/chlorosulfonylterminated silane (Yuan et al, 2009a(Yuan et al, ,b,c, 2010aYang et al, 2014) and bromomethyl-terminated biomimetic catechol (Koplin et al, 2008). Monomers containing tertiary amino groups, such as 2-dimethylaminoethyl methacrylate (DMAEMA) and 4-vinyl pyridine (4VP), have been extensively used in synthesizing antimicrobial polymers via surface graft polymerization (Figure 3) (Fan et al, 2005;Koplin et al, 2008;Yuan et al, 2009aYuan et al, ,b,c, 2010aYang et al, 2014). As an example of water-soluble polyamine, polyethyleneimine has not only been employed in preparing antimicrobial coatings (Milovic et al, 2005;Huang et al, 2008;Aguirre et al, 2017), but also been used as a corrosion inhibitor to protect steel (Braunecker and Matyjaszewski, 2007;Kugel et al, 2011;Rajasekar and Ting, 2011).…”

High antibacterial efficiency of pDMAEMA modified silicon nanowire arrays

“…Such materials are important in fields such as food packaging, water treatment, and implantable medical devices [1][2][3][4]. Because of their high stability and low environmental impact, various antibacterial polymers have been used as surface modifiers on materials such as polyolefins, chitosan, and N-dodecyl-N-methylpolyethylenimine [5][6][7]. Poly(2-(dimethylamino ethyl)methacrylate) (pDMAEMA) has been investigated extensively in this context because it is rich in tertiary amino groups which can be converted to positively charged quaternary ammonium (QA) groups with bactericidal properties.…”