Evaluation of the antifungal activity of sophorolipids from Starmerella bombicola against food spoilage fungi

Hipólito, Amanda; Silva, Ricardo Augusto Alves da; Caretta, Talita de Oliveira; Silveira, Victória Akemi Itakura; Amador, Ismael Rodrigues; Panagio, Luciano Aparecido; Borsato, Dionísio; Celligoi, Maria Antonia Pedrine Colabone

doi:10.1016/j.bcab.2020.101797

Cited by 40 publications

(34 citation statements)

References 42 publications

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“…Antimicrobial activity was first reported for lactonic SLs that inhibited growth of specific alkane utilizing yeasts (only when grown on C10 to C18 alkanes) [31]. Succeeding studies showed that SLs also inhibit bacteria (e.g., B. subtilis and P. aeruginosa) and fungi (e.g., U. maydis or foodborne Aspergillus strains) [15,[32][33][34][35][36][37]. Here, the antimicrobial properties of SLs were investigated against some standard microbial strains used for antimicrobial studies and representing different microbial groups, and three ecologically highly relevant strains.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

“…Several plausible hypotheses on the natural role of SLs in Starmerella bombicola have been postulated in the literature and can be summarized as follows: (1) SL production constitutes an overflow metabolism [30]; (2) SLs exert antimicrobial activity, thereby inhibiting the growth of competing microorganisms [15,[31][32][33][34][35][36][37]; (3) SL production is a protection mechanism against high osmotic pressure [38]; (4) SLs improve the uptake of hydrophobic substrates [39,40]; and (5) SLs serve as an extracellular storage compound of carbon and energy [38].…”

Section: Introductionmentioning

confidence: 99%

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Elucidation of the Natural Function of Sophorolipids Produced by Starmerella bombicola

Clercq

Roelants

Castelein

et al. 2021

JoF

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The yeast Starmerella bombicola distinguishes itself from other yeasts by its potential of producing copious amounts of the secondary metabolites sophorolipids (SLs): these are glycolipid biosurfactants composed out of a(n) (acetylated) sophorose moiety and a lipid tail. Although SLs are the subject of numerous research papers and have been commercialized, e.g., in eco-friendly cleaning solutions, the natural function of SLs still remains elusive. This research article investigates several hypotheses for why S. bombicola invests that much energy in the production of SLs, and we conclude that the main natural function of SLs in S. bombicola is niche protection: (1) the extracellular storage of an energy-rich, yet metabolically less accessible carbon source that can be utilized by S. bombicola upon conditions of starvation with (2) antimicrobial properties. In this way, S. bombicola creates a dual advantage in competition with other microorganisms. Additionally, SLs can expedite growth on rapeseed oil, composed of triacylglycerols which are hydrophobic substrates present in the yeasts’ environment, for a non-SL producing strain (Δcyp52M1). It was also found that—at least under lab conditions—SLs do not provide protection against high osmotic pressure prevalent in sugar-rich environments such as honey or nectar present in the natural habitat of S. bombicola.

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Section: Antimicrobial Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Elucidation of the Natural Function of Sophorolipids Produced by Starmerella bombicola

Clercq

Roelants

Castelein

et al. 2021

JoF

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“…and Pythium sp. 10 ; Colletotrichum gloeosporioides, Fusarium verticilliodes and Corynespora cassiicola 11 ; Fusarium oxysporum, Pyricularia orizae, R. solani and Phytophthora infestans 12 ; and Aspergillus ssp., B. cinerea and Rhizopus ssp., 13 the potential of sophorolipids as a substitute for synthetic pesticides is still poorly explored. Thus, the present study aimed to produce and apply the sophorolipids of S. bombicola against four tomato phytopathogens in vitro, and also conduct in vivo tests of leaves and cherry tomato fruits against B. cinerea.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of sophorolipids produced by Starmerella bombicola against phytopathogens from cherry tomato

et al. 2021

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BACKGROUND: Phytopathogenic microorganisms are the main cause of plant diseases, generating significant economic losses for the agricultural and food supply chain. Cherry tomatoes (Solanum lycopersicum var. cerasiforme) are very perishable plants and highly demanding in the use of pesticides; therefore, alternative solutions such as biosurfactants have aroused as a potent substituent. The main objective of the present study was to investigate the antimicrobial activity of sophorolipids against the phytopathogens Botrytis cinerea, Sclerotium rolfsii, Rhizoctonia solani and Pythium ultimum. RESULTS:The biosurfactant inhibited the mycelial growth in vitro with a minimum concentration of 2 mg mL −1 . The application of sophorolipids at 1, 2 and 4 mg mL −1 in detached leaves of tomato before the inoculation of the fungus B. cinerea was the best treatment, reducing leaf necrosis by up to 76.90%. The use of sophorolipids for washing tomato fruits before the inoculation of B. cinerea was able to inhibit the development of gray mold by up to 96.27%. CONCLUSION:The results for tomato leaves and fruits revealed that the biosurfactant acts more effectively when used preventively. Sophorolipids are stable molecules that show promising action for the potential replacement of pesticides in the field and the post-harvest process against the main tomato phytopathogens.

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“…Os soforolipídios pertencem a classe dos glicolipídios, são metabólitos secundários produzidos por muitas leveduras não patogênicas, a partir de açúcares e lipídeos, secretados para o meio extracelular como uma mistura de compostos de estruturas químicas relacionadas (Cooper e Paddock, 1983). O principal microrganismo produtor é a levedura não patogênica Starmerella bombicola, devido aos seus altos rendimentos de produção bastante expressivos e baixa toxicidade (Hipólito et al 2020;Silveira et al 2020).…”

Section: Introductionunclassified

“…Os soforolipídios são eficazes como agente bactericida independentemente de seu conteúdo acídico e/ou lactônico, capaz de induzir morte células planctônicas tanto de bactérias quanto de fungos, sendo comparável aos antimicrobianos convencionais (Hipólito et al 2020;Silveira, Freitas, Celligoi, 2018). Os soforolipídios interferem na formação de biofilmes em concentrações superiores a 5% (v/v) (Díaz de Rienzo et al 2015), e sua atividade antimicrobiana contra patógenos alimentares ) e bactérias degradantes em curtumes de couro (Solaiman et al 2016) já foi evidenciada quando utilizado na limpeza e sanitização nestas indústrias.…”

Section: Introductionunclassified

Soforolipídios de Starmerella bombicola como sanitizante na lavagem de carcaças por aspersão e imersão no abate e processamento de aves.

Freitas

Silveira

Borsato

et al. 2021

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Este trabalho teve como objetivo aplicar soforolipídios produzidos pela levedura Starmerella bombicola, como sanitizante, na água de lavagem de carcaças de frango, simulando as etapas do abate e processamento por aspersão e imersão de aves contra os microrganismos Staphylococcus aureus, Escherichia coli, coliformes termotolerantes e aeróbios mesófilos. Para essa avaliação foram aplicadas soluções de soforolipídios a 500, 2.250 e 4.000 µg.mL-1 para verificar o efeito antimicrobiano. Os resultados avaliados demonstraram que a sanitização por aspersão a redução mais significativa foi com a solução de soforolipídios a 4.000 µg.mL-1, com a redução de 1,32 para S. aureus; 1,47 para E. coli; 1,43 para coliformes termotolerantes e 1,05 para aeróbios mesófilos. Na água de imersão, a ação sanitizante aumentou gradativamente com o tempo de exposição. Ocorreu a redução total das células viáveis com 90 minutos com 2.250 μg.mL-1 de soforolipídios e com 30 minutos na concentração de 4.000 μg.mL-1. Os soforolipídios mostraram um potencial inovador contra os microrganismos contaminantes da indústria avícola, podendo ser um novo agente sanitizante trazendo maior segurança alimentar aos produtos da indústria avícola.

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Evaluation of the antifungal activity of sophorolipids from Starmerella bombicola against food spoilage fungi

Cited by 40 publications

References 42 publications

Elucidation of the Natural Function of Sophorolipids Produced by Starmerella bombicola

Elucidation of the Natural Function of Sophorolipids Produced by Starmerella bombicola

Antimicrobial activity of sophorolipids produced by Starmerella bombicola against phytopathogens from cherry tomato

Soforolipídios de Starmerella bombicola como sanitizante na lavagem de carcaças por aspersão e imersão no abate e processamento de aves.

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