Evaluation of the antibiotic activity of extracellular compounds produced by the Pseudomonas strain against the Xanthomonas citri pv. citri 306 strain

Oliveira, Admilton Gonçalves de; Murate, Letı́cia Sayuri; Spago, Flávia Regina; Lopes, Lucilene de Paula; Beranger, Jamile Priscila de Oliveira; Martin, Juca Abramo Barrera San; Nogueira, Marco Antônio; Mello, João Carlos Palazzo de; Andrade, Célia Guadalupe Tardeli de Jesus; Andrade, Galdino

doi:10.1016/j.biocontrol.2010.10.008

Cited by 57 publications

(40 citation statements)

References 30 publications

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“…These findings are comparable with results obtained by Oliveira et al (2011) who observed that the same F3 fraction also had high antibiotic activity against X. axonopodis pv. citri (Xcc), decreasing by 93.5% the number of lesions of citrus canker in orange leaves.…”

Section: Discussionsupporting

confidence: 91%

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Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

Lopes¹,

Oliveira²,

Beranger³

et al. 2012

Trop. plant pathol.

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Bacterial leaf blight caused by Xanthomonas axonopodis is a major problem in the production of eucalyptus seedlings. The pathogen causes leaf lesions that reduce the photosynthetic area and limit plant growth. The objective of this study was to evaluate the antibiotic activity of secondary metabolic compounds of a Pseudomonas sp. against X. axonopodis, following extraction with dichloromethane and fractionation by vacuum liquid chromatography. The antibiotic activity of an ethyl acetate phase (F3 fraction) was determined by agar diffusion tests and bioautography. The minimum inhibitory concentration of F3 fraction and the action of F3 on the cell morphology of X. axonopodis was determined by scanning electron microscopy. Electron microscopy revealed changes in exopolysaccharides and morphology of treated cells. When eucalyptus seedlings were sprayed with the F3 fraction before or after inoculation with X. axonopodis in a greenhouse experiment the number of leaf lesions was respectively 93.9% and 89.7% lower than in untreated control plants. High antibiotic activity of the F3 fraction against X. axonopodis and leaf blight suggests that it has potential to control the disease in eucalyptus seedlings.

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Section: Discussionsupporting

confidence: 91%

“…The antibiotic compounds produced by antagonistic species, when in competition, can inhibit the growth of pathogenic species. Therefore the production, purification and use of these compounds can also be a viable strategy to control plant pathogens (Harman, 2000;Oliveira et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

Lopes¹,

Oliveira²,

Beranger³

et al. 2012

Trop. plant pathol.

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“…In the previous studies was testing the effect of secondary metabolite produced by Pseudomonas aeruginosa strain LV on different phytopathogenic bacteria [8]- [10], and other pathogenic bacteria [18] [19]. In the present study was evaluate the effect secondary metabolites produced by Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 98%

Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

Murate¹,

Oliveira²,

Higashi³

et al. 2015

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This study investigated the protective effects of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was cultured in liquid medium and the supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was concentrated, filtrated, lyophilized and fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions were obtained. All fractions' activity against Xac 306 by agar well diffusion assay and minimum inhibitory concentration but in different concentrations were tested. Cytotoxicity effects were observed in all fractions in 50 µg•mL −1 concentration. The comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no genotoxic effects at tested concentrations. In plants only VLC3 showed significant results (p < 0.05), reducing the incidence of citrus canker lesions.

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“…The production, extraction and obtation of fractions with antibiotics compounds was realized the according to [14] which method was patented [18]. After treat the supernatant with dichloromethane, the phase obtained was treated with six different solvents with different polarity resulting in six different fractions as following (v/v): hexane (100; F1), dichloromethane (100; F2), ethyl acetate (100; F3), methanol (100; F4), methanol: water (1:1; F5) and water (100; F6).The six fractions was used in the next tests.…”

Section: Production Purification and Fractioned Of Secondary Metabolmentioning

confidence: 99%

“…Compounds produced by secondary metabolism of antagonist microorganisms during competition in the environment could show antimicrobial activity [13,14]. The use of secondary metabolites with antibiotic activity is against bacterial contamination during fuel ethanol production.…”

Section: Introductionmentioning

confidence: 99%

The Control of <i>Lactobacillus</i> sp. by Extracellular Compound Produced by <i>Pseudomonas aeruginosa</i> in the Fermentation Process of Fuel Ethanol Industry in Brazil

Góis¹,

Lopes-Santos²,

Beranger³

et al. 2013

JSBS

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This work evaluated the effect of secondary bacterial metabolites produced by Pseudomonas sp LV strain in control of Lactobacillus sp. population in the microcosm of the vat during ethanol fermentation. The fraction F4 produced by Pseudomonas aeruginosa was extracted with dichloromethane and fractionating by vacuum liquid chromatography obtained in a methanol phase. The evaluation of antibiotic activity of F4 fraction mixed or not with sulphuric acid and Kamoram ® . The antibiotic activity of F4 fraction was determined as well as the fermentation efficiency. Also was determined yeast cell viability, budding formation, the viability of budding cells, and number of populations of Saccharomyces cerevisiae and Lactobacillus sp. The results showed that the F4 fraction had high selective antibiotic activity against Lactobacillus sp. but not for S. cerevisae, and no inhibitory effect was observed in the fermentation process by yeast. Also F4 fraction decreased flocculation and foam formation. The F4 has an antibiotic activity against Lactobacillus sp. and should be used as an alternative to control bacteria contamination and foam and flocculation formation in the fuel ethanol fermentation process. The F4 fraction could reduce the use of antibiotics in the control of Lactobacillus sp. population during the fuel ethanol production.

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Evaluation of the antibiotic activity of extracellular compounds produced by the Pseudomonas strain against the Xanthomonas citri pv. citri 306 strain

Cited by 57 publications

References 30 publications

Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

The Control of <i>Lactobacillus</i> sp. by Extracellular Compound Produced by <i>Pseudomonas aeruginosa</i> in the Fermentation Process of Fuel Ethanol Industry in Brazil

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Evaluation of the antibiotic activity of extracellular compounds produced by the Pseudomonas strain against the Xanthomonas citri pv. citri 306 strain

Cited by 57 publications

References 30 publications

Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

Activity of extracellular compounds of Pseudomonas sp. against Xanthomonas axonopodis in vitro and bacterial leaf blight in eucalyptus

Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

The Control of &lt;i&gt;Lactobacillus&lt;/i&gt; sp. by Extracellular Compound Produced by &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; in the Fermentation Process of Fuel Ethanol Industry in Brazil

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The Control of <i>Lactobacillus</i> sp. by Extracellular Compound Produced by <i>Pseudomonas aeruginosa</i> in the Fermentation Process of Fuel Ethanol Industry in Brazil