Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

Murate, Letı́cia Sayuri; Oliveira, Admilton Gonçalves de; Higashi, Allan Yukio; Barazetti, André Riedi; Simionato, Ane Stéfano; Silva, Caroline Santos da; Simões, Glenda Cavalari; Santos, Igor Matheus Oliveira dos; Ferreira, Marlon Renan; Cely, Martha Viviana Torres; Navarro, Miguel Octavio Pérez; Freitas, Vanessa Fogaça de; Nogueira, Marco Antônio; Mello, João Carlos Palazzo de; Leite, Rui Pereira; Andrade, Galdino

doi:10.4236/as.2015.63030

Cited by 15 publications

(16 citation statements)

References 18 publications

(18 reference statements)

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“…The chemotherapeutic potential of Fluopsin C has been highlighted since its first description, but the significant cytotoxicity against Ehrlich, Hela, and sarcoma 180 cells line, added to acute toxicity in animal models, suggested its unfeasibility to control infections in humans (Itoh et al, 1970; Otsuka et al, 1972). Fluopsin C also increased CC 50 / 24 h when compared to previous studies with semi-purified fractions, where the cytotoxicity was not detected with F3 and F3d fractions (Murate et al, 2015; Kerbauy et al, 2016). Probably, the activity of these fractions against LLCMK2 cell line was low because Fluopsin C was diluted in these fractions.…”

Section: Discussioncontrasting

confidence: 53%

“…The development of molecules complexed with metals as potential medicinal agents has been rising the last decade (Cardozo et al, 2013; de Oliveira et al, 2016; Kerbauy et al, 2016; Munhoz et al, 2017; Stringer et al, 2017). Previous studies demonstrated that specific fractions obtained from the culture of Pseudomonas aeruginosa LV strain (F3, F3D, and F4A) present very strong antimicrobial activity against many different pathogens (de Oliveira et al, 2011, 2016; Cardozo et al, 2013; Murate et al, 2015; Kerbauy et al, 2016; Munhoz et al, 2017; Simionato et al, 2017). Indeed, our research group demonstrated the strong antibiotic activity of these semi-purified fractions against planktonic cells and biofilm formation of MDR isolates (Kerbauy et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

et al. 2019

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The increasing emergence of multidrug-resistant (MDR) organisms in hospital infections is causing a global public health crisis. The development of drugs with effective antibiotic action against such agents is of the highest priority. In the present study, the action of Fluopsin C against MDR clinical isolates was evaluated under in vitro and in vivo conditions. Fluopsin C was produced in cell suspension culture of Pseudomonas aeruginosa LV strain, purified by liquid adsorption chromatography and identified by mass spectrometric analysis. Bioactivity, bacterial resistance development risk against clinically important pathogenic strains and toxicity in mammalian cell were initially determined by in vitro models. In vivo toxicity was evaluated in Tenebrio molitor larvae and mice. The therapeutic efficacy of intravenous Fluopsin C administration was evaluated in a murine model of Klebsiella pneumoniae (KPC) acute sepsis, using six different treatments. The in vitro results indicated MIC and MBC below 2 μg/mL and low bacterial resistance development frequency. Electron microscopy showed that Fluopsin C may have altered the exopolysaccharide matrix and caused disruption of the cell wall of MDR bacteria. Best therapeutic results were achieved in mice treated with a single dose of 2 mg/kg and in mice treated with two doses of 1 mg/kg, 8 h apart. Furthermore, acute and chronic histopathological studies demonstrated absent nephrotoxicity and moderate hepatotoxicity. The results demonstrated the efficacy of Fluopsin C against MDR organisms in in vitro and in vivo models, and hence it can be a novel therapeutic agent for the control of severe MDR infections.

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Section: Discussioncontrasting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

et al. 2019

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“…The authors observed a bacteriolytic effect on X. citri without any sign of phytotoxicity (de . In addition, other secondary metabolites from P. aeruginosa prevented canker formation at low micromolar concentration (Murate et al 2015;Spago et al 2014). However, as P. aeruginosa is an opportunistic human pathogen, the use this bacterium as a biocontrol agent presents risks and, therefore, needs to be strictly regulated.…”

Section: Biological Controlmentioning

confidence: 99%

Xanthomonas citri subsp. citri: host interaction and control strategies

Martins

Andrade

Benedetti

et al. 2020

Trop. plant pathol.

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Citrus is one of the most ancient fruit crops cultivated in the world. For many countries, citrus production is an important source of revenues. However, despite the richness of citrus production worldwide, fruit yields are constantly threatened by diseases that cause serious economic and social impacts to growers and consumers. One such example is citrus canker, a disease that affects all commercial citrus varieties and for which there is no cure. Currently, control measures for citrus canker are restricted to the application of copper-based compounds and the elimination of infected trees to prevent pathogen spreading in the field. However, new alternatives for the control of this disease are being developed, spanning through transgenic plants to innovative chemicals and biological control. New genome editing approaches and a repertoire of plant defense-related genes have been exploited to produce citrus lineages more tolerant to X. citri. In addition, a series of new molecules have been tested to not only inhibit X. citri growth but to also boost the plant immune system to suppress disease progression. In this review, we provide the state of art of all citrus canker control measures in use today, highlighting their utility and drawbacks and commenting on novel strategies to better control this important citrus disease.

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“…At each stage, the Erlenmeyer flask was shaken with slow rotation. The ethyl acetate fraction was concentrated using a rotatory evaporator and the crude extract was then dissolved in dimethyl sulfoxide (DMSO) at a concentration of 1 mg/ml and stored at 4°C [24].…”

Section: Preparation Of Quorum Quencher Endophytic Bacterial Extractsmentioning

confidence: 99%

Quorum‐quenching endophytic bacteria inhibit disease caused byPseudomonas syringaepv. syringae inCitruscultivars

et al. 2020

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Two strains of 64 endophytic bacteria, Bacillus cereus Si‐Ps1 and Pseudomonas azotoformans La‐Pot3‐3, isolated from Citrus sinensis and C. sinensis var. Thomson's leaves, respectively, reduced N‐acyl homoserine‐based quorum sensing in bioindicator strain Pseudomonas syringae pv. syringae (Pss) B728a and the biofilm production and swarming motility of field isolate Pss 3289. A homolog of aiiA gene encoding an AHL‐lactonase was found in B. cereus (Si‐Ps1), suggesting that this isolate can degrade the quorum‐sensing signal molecules of Pss 3289. The crude extract of endophytic bacterium, B. cereus (Si‐Ps1), inhibited Pss 3289 biofilm formation after 48 and 96 h by 55% and 58%, respectively. Similar reductions in biofilm formation were conferred by crude extracts of P. azotoformans (La‐Pot3‐3). Correspondingly, the number of planktonic cells in cultures treated with these extracts was higher than in control cultures, indicating a direct effect on biofilm formation and not on cell growth. In greenhouse assays, the virulence of Pss 3289 to different citrus cultivars was decreased when coinoculated with these endophytic bacteria.

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Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

Cited by 15 publications

References 18 publications

Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

Xanthomonas citri subsp. citri: host interaction and control strategies

Quorum‐quenching endophytic bacteria inhibit disease caused byPseudomonas syringaepv. syringae inCitruscultivars

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