Evaluation of the anthelmintic activity of Khaya senegalensis extract against gastrointestinal nematodes of sheep: in vitro and in vivo studies

Ademola, Isaiah Oluwafemi; Fagbemi, Benjamin O.; Idowu, Olakunle S

doi:10.1016/j.vetpar.2004.04.001

Cited by 85 publications

(47 citation statements)

References 4 publications

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“…The ethanolic extract was more active than the aqueous extract (P < 0.05, Table 1). The activity of the aqueous extract is comparable with the activity shown by aqueous extract of Khaya senegalensis against infective larvae of sheep nematodes, with LC 50 of 0.69 mg/mL as recently reported (Ademola et al;.…”

Section: Discussionsupporting

confidence: 84%

“…Individual faecal egg counts were determined according to genus by larval culture to determine the genus of strongyles. Test tubefilter paper technique (Ademola et al; was used for larval culture. Briefly, 400 mg of faeces containing the eggs was weighed, this was smeared evenly and thinly on the upper two third of a prepared rectangular strips of Whatman filter paper with a spatula.…”

Section: Treatment and Follow-up Proceduresmentioning

confidence: 99%

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Anthelmintic Efficacy Of Nauclea latifolia Extract Against Gastrointestinal Nematodes Of Sheep:In vitro And In vivo Studies

Ademola¹,

Fagbemi²

2008

Afr. J. Trad. Compl. Alt. Med.

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Direct effects of Nauclea latifolia extracts on different gastrointestinal nematodes of sheep is described. In vivo and in vitro studies were conducted to determine possible anthelmintic effect of leaf extracts of Nauclea latifolia toward different ovine gastro intestinal nematodes. A larval development assay was used to investigate in vitro, the effect of aqueous and ethanolic extracts of N. latifolia towards strongyles larvae. The development and survival of infective larvae (L 3 ) was assessed and best-fit LC 50 values were computed by global model of non-linear regression analysis curve-fitting (95% CI). Twenty sheep harbouring naturally acquired gastrointestinal nematodes were treated with oral administration of ethanolic extracts at a dose rate of 125 mg/kg, 250 mg/kg and 500mg/kg to evaluate therapeutic efficacy, in vivo.The presence of the extracts in the cultures decreased the survival of larvae. The LC 50 of aqueous and ethanolic extract were 0.704 and 0.650 mg/ml respectively and differ significantly (P<0.05, paired t test). Faecal egg counts (FEC) on day 12 after treatment showed that the extract is effective, relative to control (1-way ANOVA, Dunnett's multiple comparison test), at 500mg/kg against Haemonchus spp, Trichostrongylus spp (p<0.05), Strongyloides spp (P < 0.01); at 250mg/kg against Trichuris spp (P < 0.01) and ineffective against Oesophagostomum spp (p>0.05). The effect of doses is extremely significant; the day after treatment is sometimes significant while interaction between dose and day after treatment is insignificant (2-way ANOVA). N. latifolia extract could therefore find application in the control of helminth in livestock, by the ethnoveterinary medicine approach.

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Section: Discussionsupporting

confidence: 84%

Section: Treatment and Follow-up Proceduresmentioning

confidence: 99%

Anthelmintic Efficacy Of Nauclea latifolia Extract Against Gastrointestinal Nematodes Of Sheep:In vitro And In vivo Studies

Ademola¹,

Fagbemi²

2008

Afr. J. Trad. Compl. Alt. Med.

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“…However, the efficacy of anthelmintics is increasingly endangered by the development of resistance in nematode populations (MILLER et al, 2012). The intensification of the animal production system has led to an increasing demand for effective and low-cost anthelmintic drugs to control helminth diseases (ADEMOLA et al, 2004). These concerns have led to the search for and evaluation of alternative control methods (ATHANASIADOU et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic activity of Cymbopogon citratus against Haemonchus contortus

Macedo

Oliveira

Ribeiro

et al. 2015

Rev. Bras. Parasitol. Vet.

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Parasitic nematodes are of major economic importance in livestock. An alternative for the control of parasites is phytotherapy. This study evaluated the efficacy of Cymbopogon citratus decoction (CcD), C. citratus essential oil (CcEo) and citral against Haemonchus contortus using in vitro egg hatch test (EHT) and larval development test (LDT) and an in vivo test using a Meriones unguiculatus (gerbil) model. The effect of 800 mg/kg CcEo was evaluated in gerbils that had been artificially infected with 5,000 third-stage H. contortus larvae. The effective concentrations required to inhibit 50% (EC50) of egg hatching were 0.46, 0.14 and 0.13 mg/mL for CcD, CcEo and citral, respectively. The EC50 values in the LDT were 5.04, 1.92 and 1.37 mg/mL for CcD, CcEo and citral, respectively. H. contortus population in the group treated with C. citratus essential oil was reduced by 38.5% (P< 0.05) in comparison to the control group. These results suggest that it may be possible to use C. citratus essential oil to control of H. contortus parasite of small ruminant.Keywords: Phytotherapy, anthelmintic, Meriones unguiculatus, in vitro, citral. ResumoO parasitismo por nematoides tem grande importância econômica no rebanho. Uma alternativa para o controle de parasitas é a fitoterapia. Este estudo avaliou a eficácia do decocto de Cymbopogon citratus (DCc), do óleo essencial de C. citratus (OECc) e do citral contra Haemonchus contortus utilizando o teste in vitro de eclosão dos ovos (TEO) e o teste de desenvolvimento larval (TDL) e um teste in vivo com modelo Meriones unguiculatus (gerbil). O efeito de 800 mg/kg de OECc foi avaliado em gerbils infectados artificialmente com 5000 larvas de terceira fase de H. contortus. As concentrações efetivas necessárias para inibir 50% (CE50) da eclosão dos ovos foram 0,46; 0,14 e 0,13 mg/mL para DCc, OECc e citral, respectivamente. Os valores da CE50 no TDL foram de 5,04; 1,92 e 1,37 mg/mL para DCc, OECc e citral, respectivamente. No grupo tratado com óleo de C. citratus a população H. contortus foi reduzida em 38,5% (P < 0,05) em comparação com o grupo controle. Estes resultados sugerem que pode ser possível a utilização de óleo essencial de C. citratus para controle de H. contortus, parasita de pequenos ruminantes.Palavras-chave: Fitoterapia, anti-helmínticos, Meriones unguiculatus, in vitro, citral.

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“…Juss. : A study showed that the bark of K. senegalensis possesses anthelmintic activity (Ademola et al, 2004). It is interesting to note that it is also the use of the bark of the plant that occurred during our investigation.…”

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confidence: 66%

Ethnoveterinary management of cattle helminthiasis among the Fulani and the Mossi (Central Burkina Faso): plants used and modes of use

Hilou¹,

Rappez²,

Duez³

2015

Int. J. Bio. Chem. Sci

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Because of the weakness of the modern veterinarian services in Burkina Faso, ethnoveterinary medicines are frequently used to control cattle diseases like helminthe parasites. Ethnobotanic survey was performed in the central region of Burkina Faso with 22 livestock breeders on their helminthiasis management, and traditional treatments. Differences in knowledge of the disease, in treatments and in plant species used were evaluated. The relationship between informants and herbs was graphed as an interaction network. According to the survey results helminthic diseases (recognized by piloerection, lack of appetite and weight loss) are caused by water and pasture qualities. Ten single-herb preparations and 8 herbal recipes were reported. Among the 13 plant species cited, 4 are very frequently used to control helminthiasis: Mitragyna inermis (Wild.) O. Ktze, Vitellaria paradoxa Gaertn., Acacia macrostachya Rchb. ex DC., Combretum glutinosum Perr. ex DC. Ethnoveterinary practices are an economic necessity for small-scale livestock owners in rural regions of Burkina Faso. Probably, the most used species can provide, after phytochemical analysis, molecules of pharmaceutical interest. There also appears a definite need for more specific diseases diagnostics that will help to improve helminthiasis control by farmers.

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Evaluation of the anthelmintic activity of Khaya senegalensis extract against gastrointestinal nematodes of sheep: in vitro and in vivo studies

Cited by 85 publications

References 4 publications

Anthelmintic Efficacy Of <i>Nauclea latifolia</i> Extract Against Gastrointestinal Nematodes Of Sheep:<i>In vitro</i> And <i>In vivo</i> Studies

Anthelmintic Efficacy Of <i>Nauclea latifolia</i> Extract Against Gastrointestinal Nematodes Of Sheep:<i>In vitro</i> And <i>In vivo</i> Studies

Anthelmintic activity of Cymbopogon citratus against Haemonchus contortus

Ethnoveterinary management of cattle helminthiasis among the Fulani and the Mossi (Central Burkina Faso): plants used and modes of use

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