Evaluation of System Errors in TLC-Densitometry when using the Streaking Technique

Costanzo, Samuel J.; Cardone, Mario J.

doi:10.1080/01483918408067038

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1988

1996

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Cited by 6 publications

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“…Because of the optical medium, a nonlinear response is usually observed for direct densitometry measurements (34,35). This can be corrected by applying the Kubelka-Munk function.…”

Section: Resultsmentioning

confidence: 99%

Laser-based indirect fluorometric detection and quantification in thin-layer chromatography

Ma¹,

Koutny²,

Yeung³

1989

Anal. Chem.

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A laser-based Indirect fluorometric detectlon method for thin-layer chromatography Is described. The new technique can be easlty used for quantttatlve measurements because of Its two-dimensional scanning capability. Since It Is based on the Indirect fluorescence mode, unlversai detection Is posslble wIthout derlvatizatlon. Also, it takes only 35 s for acqulrlng a data array of 256 X 64 points with thIs technique to achleve a detection llmtt of 6 pg. Thls Is 100 times lower than when the human eye Is used as a detector for the same samples based on lndlrect fluorometry. The lineartty in indirect fluorometric detectlon Is found to be over 2 orders of magnltude.

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“…Because of the optical medium, a nonlinear response is usually observed for direct densitometry measurements (34,35). This can be corrected by applying the Kubelka-Munk function.…”

Section: Resultsmentioning

confidence: 99%