Laser-based indirect fluorometric detection and quantification in thin-layer chromatography

Ma, Yinfa; Koutny, Lance; Yeung, Edward S.

doi:10.1021/ac00192a031

Cited by 6 publications

References 32 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Analytical Methods, Trends

Montana¹

2000

Kirk-Othmer Encyclopedia of Chemical Technology

View full text Add to dashboard Cite

Analytical chemistry, the science of the measurement and characterization of systems, is being revolutionized by rapid advances in computer technology, microelectronics, and materials science. These developments are being driven by five factors: the quest for truly automated or intelligent instruments; ie, the continued development of multidimensional hyphenated instruments, the marriage of two instruments having complementary capabilities to facilitate the analysis of complex samples; continuous improvements in analyte detection limits; increased capabilities in data manipulation, hardware miniaturization, and remote in vivo sensing, leading to instrumentation capable of both separation and specification; and the development of nondestructible, stable, field‐operable instrumentation utilizing fiber optic technology as in‐field sampling devices and for process control. It is becoming more desirable for the analytical chemist to move into the field via in‐field instruments and remote, point‐of‐use measurements. In situ analysis enables the study of key process parameters. On‐line analysis capability has already been extended to gc, lc, ms, and ftir techniques as well as to icp‐emission spectroscopy, flow injection analysis, and near‐infrared spectrophotometry. New technology developments such as on‐line Raman spectroscopy, x‐ray diffraction, and gc/lc were commercialized during the 1990s. One challenge is the migration of analytical techniques out of research and development, into quality control and process control. Another involves continued advances in smaller sample size, higher sensitivity and specificity and achieving lowered costs per analysis.

show abstract

Analytical Methods, Trends

Montana¹

2000

Kirk-Othmer Encyclopedia of Chemical Technology

View full text Add to dashboard Cite

show abstract

Quantitative determination of ionized calcium and total calcium in human serum by capillary zone electrophoresis with indirect photometric detection

Zhang

Shi

1994

J Microcolumn Separations

View full text Add to dashboard Cite

Abstract. We have developed a new method to quantitatively determine ionized calcium and total calcium by using high performance capillary electrophoresis (HPCE) with indirect photometric detection. Other cations such as Na+, K+, and Mg+ can also be quantitatively determined. A 70 cm x 50 pm i.d. fused silica capillary was used for the separation. The detection window was located 25 cm from the cathodic end, and the detection wavelength was set at 214 nm. A 100 mM imidazole solution at pH 7.4(adjusted with 5 % H2S04) containing 0.1 % hydroxypropylmethylcellulose (HMC, 4000 centipoise) was used as background electrolyte. Whole serum was injected for 5 s at 15 kV for the determination of ionized calcium. The total calcium levels in a number of human serum samples were determined by HPCE and compared with the NIST reference method (atomic absorption), and the results agree with each other within 95 % confidence. Two NIST standards were analyzed by HPCE, and the results fit each other perfectly. The ionized calcium levels in sera by this HPCE method have been compared with those obtained with ion-selective electrode (ISE) method, and the results were lower than those of ISE, which was what we anticipated. The effects of sample temperature and buffer pH were studied.

show abstract

Multidimensionality in planar chromatography

Poole

1995

Journal of Chromatography A

View full text Add to dashboard Cite

Laser-based indirect fluorometric detection and quantification in thin-layer chromatography

Cited by 6 publications

References 32 publications

Analytical Methods, Trends

Analytical Methods, Trends

Quantitative determination of ionized calcium and total calcium in human serum by capillary zone electrophoresis with indirect photometric detection

Multidimensionality in planar chromatography

Contact Info

Product

Resources

About