Evaluation of synuclein-γ levels by novel monoclonal antibody in saliva and cancer tissues from oral squamous cell carcinoma patients

Wang, K; Shen, Yaoyuan; Xu, Jianmin; Li, Z; Liu, Y; Chen, Yu; Peng, L; Zheng, Jun; Zeng, Yan

doi:10.4149/neo_2020_190619n523

Cited by 9 publications

(7 citation statements)

References 29 publications

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“…In this study, we explored the expression and role of SNCG in OSCC. First, we detected the mRNA and protein expression of SNCG in OSCC and found that SNCG was highly expressed in OSCC tissues, which was consistent with our previous study [ 24 , 25 ]. And our results were consistent with those reported in multiple articles, which indicate that SNCG was highly expressed in a variety of tumors [ 9 , 37 , 38 ].…”

Section: Discussionsupporting

confidence: 90%

“…Therefore, the difference between our experimental results and database results may be due to different sample sources and ethnic differences, which may require more samples to test. At the same time, we analyzed the correlation between the expression of SNCG in OSCC and clinicopathological features and found that the expression of SNCG was related to differentiation, which was consistent with our previous study [ 24 , 25 ]. Next, we explored the diagnostic value of SNCG in OSCC tissues.…”

Section: Discussionsupporting

confidence: 90%

“…Our previous study has found that the levels of SNCG in saliva and serum of OSCC patients are higher than those of patients with oral potential malignant diseases and healthy controls and are related to the differentiation of OSCC [ 24 , 25 ]. On the basis of previous studies, we intended to further explore the expression of SNCG in OSCC tissues and its value in the diagnosis and prognosis of OSCC.…”

Section: Introductionmentioning

confidence: 99%

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Upregulation of Synuclein-γ and Snai1 Contributes to Poor Clinical Prognosis in Oral Squamous Cell Carcinoma Patients

Yang

Pan

Peng

et al. 2022

BioMed Research International

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Synuclein-γ (SNCG) and Snai1 play an important role in the occurrence and development of different types of malignant tumors. However, the association between SNCG and Snai1 and the effect of their combination on oral squamous cell carcinoma (OSCC) are unknown. The purpose of this study was to assess the expression of SNCG and Snai1 in OSCC tissues and their role in the genesis, development, diagnosis, and prognosis of OSCC. In this study, we first analyzed the Gene Expression Omnibus (GEO) database to determine the expression of SNCG and Snai1 in OSCC. And we also evaluated the correlation between the expression of SNCG and Snai1 and clinical pathological parameters in OSCC from The Cancer Genome Atlas (TCGA) database. Then, the expression of SNCG and Snai1 in OSCC and its adjacent tissues in our experimental cohort were detected by qRT-PCR, Western blot, and immunohistochemistry, and the relationship between their expression and clinical pathological parameters were analyzed. At the same time, the correlation between the expression of SNCG and Snai1 was analyzed from the TCGA, GEO database, and our experimental cohort. Next, the ROC curves were constructed to explore the diagnostic value of SNCG and Snai1 in OSCC. Finally, the survival curves were drawn, and the univariate and multivariate Cox regression analyses were performed to determine the prognostic value of SNCG and Snai1 in OSCC. The study found that SNCG and Snai1 were highly expressed in OSCC tissues. The expression of SNCG was related to the differentiation of OSCC, while that of Snai1 was related to the T stage, lymph node metastasis, clinical stage, and differentiation. Besides, the expression of SNCG in OSCC was positively correlated with that of Snai1. In addition, we also found that SNCG and Snai1 could well distinguish OSCC patients from normal people; especially, the combined diagnosis of SNCG and Snai1 had a better effect, with a specificity up to 96.67%. Moreover, SNCG-negative/Snai1-negative OSCC patients had the best prognosis. Multivariate analysis displayed that SNCG-positive expression was an independent risk factor for prognosis in OSCC patients. The results of this study strongly suggested that SNCG and Snai1 might have a cooperative effect in the occurrence and development of OSCC. They may become new markers for the diagnosis and prognosis of OSCC.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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Upregulation of Synuclein-γ and Snai1 Contributes to Poor Clinical Prognosis in Oral Squamous Cell Carcinoma Patients

Yang

Pan

Peng

et al. 2022

BioMed Research International

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“…SNCG is elevated in OSCC cells and increases the ability of the human OSCC cells, OEC‐M1, to migrate and invade 10 . Our previous study has confirmed the existence of SNCG in OSCC cells and shown high levels of salivary SNCG in OSCC and OPMD patients 28 . Nevertheless, it cannot distinguish between these two types of patients.…”

Section: Discussionmentioning

confidence: 74%

Assessment of serum synuclein‐γ and squamous cell carcinoma antigen as diagnostic biomarkers in patients with oral squamous cell carcinoma and oral potentially malignant disorders

Chen

Luo

Yang

et al. 2020

J Oral Pathology Medicine

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Background Clinical diagnosis and monitoring are crucial to reduce the mortality from oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs). It has been demonstrated that synuclein‐γ (SNCG) and squamous cell carcinoma antigen (SCCAg) are highly expressed in patients with OSCC and perhaps participate in OSCC progression. This study analyzed the levels of serum SNCG and SCCAg in OSCC, OPMD, and control patients, and evaluated the diagnostic and clinical value of single and combined detection of serum SNCG and SCCAg in OSCC and OPMDs. Patients and methods Serum samples were collected from 197 patients including 87 patients with OSCC, 30 patients with OPMDs, and 80 healthy volunteers as controls. Enzyme‐linked immunosorbent assay and statistical analysis were utilized to determine SNCG and SCCAg levels in serum. Results The levels of SNCG and SCCAg in serum were significantly higher in OSCC compared with OPMDs and controls. There was a correlation between SNCG level and ethnicity, and SCCAg was correlated with differentiation. Furthermore, the area under the curves, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of combined detection of SNCG and SCCAg were better than any single detection. Conclusion The combined detection of SNCG and SCCAg in serum could become a new standard method to distinguish between OSCC and OPMDs and improve diagnostic performance for OSCC.

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“…2 mL of fasting whole blood was collected as a blood sample, centrifuged at 1200 g for 10 min at room temperature (RT), and transferred the clear supernatant as the serum sample to sterile test tubes. Subjects were asked to rinse their mouth with water for 5 minutes before the saliva sample was collected.1 ~ 2 mL of saliva was obtained by naturally secreted into the sterile collection tube, the sample was immediately centrifuged at 1000 g for 2 min at RT, and the supernatant was transferred to a sterile sample tube to be mixed with protease inhibitor [15]. All saliva and serum samples were stored in the refrigerator at -80℃.…”

Section: Serum and Saliva Samples Collectionmentioning

confidence: 99%

SPP1 as a potential biomarker for diagnosis and prognosis in oral squamous cell carcinoma

Sun

Peng

Zeng

et al. 2022

Preprint

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Background:Our aim was to evaluate the clinical significance of SPP1 in OSCC tissues and to detect the clinical diagnostic value of SPP1 in serum and saliva of OSCC. Methods:The expression of SPP1 was predicted by TCGA database, and the correlation between SPP1 expression and prognosis of OSCC was evaluated. The levels of SPP1 in 90 OSCC tissues and 40 normal tissues were detected by immunohistochemistry. ELISA was utilized to determine SPP1 levels in serum and saliva containing 70 OSCC patients and 20 healthy persons.Statistical analysis was used to determine correlations between SPP1 expression and clinicopathological characteristics, generate survival curves and analyze diagnostic efficiency. Results：TCGA database showed the SPP1 in OSCC tissues was higher than in normal tissues, patients with high SPP1 expression in OSCC had a shorter survival time. The results of immunohistochemistry were consistent with TCGA database. SPP1 in OSCC tissues and saliva were related with tumor differentiation and tumor stage. The diagnostic value of SPP1 in saliva was higher than that in serum. Conclusions: The expression of SPP1 was significantly increased in OSCC tissues, serum and saliva.. High expression of SPP1 may play an important role in diagnosis and prognosis of OSCC.

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Evaluation of synuclein-γ levels by novel monoclonal antibody in saliva and cancer tissues from oral squamous cell carcinoma patients

Cited by 9 publications

References 29 publications

Upregulation of Synuclein-γ and Snai1 Contributes to Poor Clinical Prognosis in Oral Squamous Cell Carcinoma Patients

Upregulation of Synuclein-γ and Snai1 Contributes to Poor Clinical Prognosis in Oral Squamous Cell Carcinoma Patients

Assessment of serum synuclein‐γ and squamous cell carcinoma antigen as diagnostic biomarkers in patients with oral squamous cell carcinoma and oral potentially malignant disorders

SPP1 as a potential biomarker for diagnosis and prognosis in oral squamous cell carcinoma

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