SPP1 as a potential biomarker for diagnosis and prognosis in oral squamous cell carcinoma

Abstract

Background:Our aim was to evaluate the clinical significance of SPP1 in OSCC tissues and to detect the clinical diagnostic value of SPP1 in serum and saliva of OSCC. Methods:The expression of SPP1 was predicted by TCGA database, and the correlation between SPP1 expression and prognosis of OSCC was evaluated. The levels of SPP1 in 90 OSCC tissues and 40 normal tissues were detected by immunohistochemistry. ELISA was utilized to determine SPP1 levels in serum and saliva containing 70 OSCC patients and 20 healthy persons.Statistical analysis was used to determine correlations between SPP1 expression and clinicopathological characteristics, generate survival curves and analyze diagnostic efficiency. Results：TCGA database showed the SPP1 in OSCC tissues was higher than in normal tissues, patients with high SPP1 expression in OSCC had a shorter survival time. The results of immunohistochemistry were consistent with TCGA database. SPP1 in OSCC tissues and saliva were related with tumor differentiation and tumor stage. The diagnostic value of SPP1 in saliva was higher than that in serum. Conclusions: The expression of SPP1 was significantly increased in OSCC tissues, serum and saliva.. High expression of SPP1 may play an important role in diagnosis and prognosis of OSCC.