Evaluation of ^99mTechnetium/⁵¹Chromium Post‐Transfusion Recovery of Red Cells Stored in Saline, Adenine, Glucose, Mannitol for 42 Days¹

Abstract

An in vivo and in vitro study of 42-day saline, adenine, glucose, mannitol (SAGM) red cells (Terumo Corporation, Elkton, Md.) was conducted using 20 volunteers to document in vivo efficacy and analyze the validity of the 99mTc/51Cr technique. In vivo autologous post-transfusion recovery was measured by a double-label procedure involving the use of 99mTc-labeled freshly drawn red cells to quantify recipient blood volume and 51Cr to document both post-transfusion 24-hour recovery (PTR) and red cell survival. As an internal control, 5 of the 20 donors studied had red cell volumes estimated by the 125I-albumin (RISA) plasma volume technique on a separate occasion. For comparison, PTR was also calculated according to the single-label 51Cr protocol in which recipient blood volume was estimated by extrapolation from circulating 51Cr-labeled red cell activity 5-15 min after infusion. After 42 days of storage, PTR averaged 78 +/- 6% with the double-label 99mTc/51Cr technique and 81 +/- 5% with the single-label (51Cr alone), confirming a small difference between the two techniques. Correlation between the two techniques was high, r = 0.81, though the average 3% difference between them was significant (p less than 0.05). Red cell volumes of the 5 donors measured by both the 99mTc-red cell method and the 125I-albumin method exhibited excellent correlation, r = 0.96, and averaged 1,961 +/- 420 ml and 2,048 +/- 381 ml, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Comparison of radioisotope methods and a nonradioisotope method to measure the RBC volume and RBC survival in the baboon

“…The elution of 51 Cr, 99m Tc, and 111 In‐oxine from autologous liquid preserved RBCs reported by Marcus and associates 12 may have been affected by the pH of the RBCs at the time of labeling. Heaton and associates, 34 AuBuchon and associates, 35 and Beutler and West 36 have utilized the double‐label procedure ( 51 Cr and 99m Tc) to measure the 24‐hour posttransfusion survival of autologous preserved RBCs. In this double‐label procedure, 99m Tc is used to label the fresh autologous RBCs to measure the RBC volume of the recipient and 51 Cr is used to label the autologous preserved RBCs.…”

Viability and in vitro properties of AS‐1 red cells after gamma irradiation

“…The double‐label approach uses the 99m Tc level after infusion of labeled cells to assess the recipient's RBC volume. The circulating 51 Cr level is determined by calculation that includes the amount of 51 Cr infused 10 …”

Prospective, Paired Crossover Comparison of the in vitro Quality of Red Blood Cells Collected by the Automate for Blood Collection Device or by a Conventional Method

“…Modern whole blood collecting systems and additive solutions allow for storage of RBC up to 42 or 49 days with acceptable in vivo survival rates [8,9].…”