Viability and in vitro properties of AS‐1 red cells after gamma irradiation

Moroff, Gary; Holme, Stein; AuBuchon, James P.; Heaton, W. A. L.; Sweeney, Joseph D.; Friedman, Leonard I.

doi:10.1046/j.1537-2995.1999.39299154725.x

Cited by 80 publications

(89 citation statements)

References 18 publications

(30 reference statements)

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“…In addition, potassium leakage from irradiated red cells is significantly enhanced. 12 Although this results in only a small total amount of potassium infused into a recipient, the potential for adverse effects in neonates and infants has been considered. In this regard, some hospitals have implemented additional limits on the storage time for irradiated RBCs transfused into neonates, particularly in the setting of hyperkalemia and/or renal failure.…”

Section: The Problems and Challenges Of Blood Component Irradiationmentioning

confidence: 99%

Irradiation eradication and pathogen reduction. Ceasing cesium irradiation of blood products

Mintz

Wehrli

2009

Bone Marrow Transplant

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Section: The Problems and Challenges Of Blood Component Irradiationmentioning

confidence: 99%

Irradiation eradication and pathogen reduction. Ceasing cesium irradiation of blood products

Mintz

Wehrli

2009

Bone Marrow Transplant

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“…[21][22][23] Therefore, the identification of cases warranting irradiation remains important. Current guidelines for irradiation of cellular blood components are largely driven by the immune status of the recipient, with the common exception of the irradiation of HLA-matched platelet transfusions.…”

Section: Introductionmentioning

confidence: 99%

A systematic review of transfusion-associated graft-versus-host disease

Kopolovic¹,

Ostro²,

Tsubota

et al. 2015

Blood

148

163

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• Fifty percent of TA-GVHD cases occur in patients who would not be predicted to be at risk for TA-GVHD by current guidelines for blood irradiation.• Donor lymphocytes whose HLA antigens are all shared by the recipient dominate in TA-GVHD cases, particularly in immune-competent recipients.Transfusion-associated graft-versus-host disease (TA-GVHD) is a rare complication of blood transfusion. The clinicolaboratory features of TA-GVHD and the relative contributions of recipient and component factors remain poorly understood. We conducted a systematic review of TA-GVHD reports. The HLA relationship between donor and recipient was classified as D 5 0 when no donor antigens were foreign to the recipient vs D ‡ 1 when ‡1 donor antigen disparity occurred. We identified 348 unique cases. Criteria for component irradiation were met in 48.9% of cases (34.5% immune-compromised, 14.4% related-donor), although nonirradiated components were transfused in the vast majority of these (97.6%). Components were typically whole blood and red cells. When reported, component storage duration was £10 days in 94%, and 23 (6.6%) were leukoreduced (10 bedside, 2 prestorage, and 11 unknown). Among 84 cases with HLA data available, the category of D 5 0 was present in 60 patients (71%) at either HLA class I or II loci and was more common among recipients without traditional indications for component irradiation. These data challenge the historic emphasis on host immune defects in the pathogenesis of TA-GVHD. The dominant mechanism of TA-GVHD in both immunocompetent and compromised hosts is exposure to viable donor lymphocytes not recognized as foreign by, but able to respond against, the recipient. (Blood. 2015;126(3):406-414)

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“…chemotherapy and frequent drawing of blood) certainly had an influence on RBC recovery and survival time. This might also explain that the SD value was comparatively higher than in published studies with healthy volunteers [27] and that the 24-hour RBC recovery in some patients was less than 75% (range 62-98%). The longest interval that could be investigated was 67 days.…”

Section: ----------------------------------------------------------mentioning

confidence: 62%

Flow-Cytometric Determination of Survival Time and 24-Hour Recovery of Transfused Red Blood Cells

Zeiler¹,

Müller²,

Kretschmer³

2003

Transfus Med Hemother

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Introduction: We tested the feasibility of the determination of 24-hour recovery and survival time of transfused red blood cells (RBCs) by flow cytometry to evaluate a new RBC preparation in a clinical application study. Patients and Methods: Eleven patients received 22 inline filtered RBC concentrates from apheresis (mean storage time of transfused RBCs 25.6 ± 12 days). Recovery and survival were calculated based on flow-cytometric determination of autologous and transfused RBCs by measuring antigens differing between donor and recipient. Human polyclonal erythrocyte antibodies (IgG) were used as primary and FITC-conjugated rabbit antihuman IgG antibody (F(ab’)₂) fragments as secondary antibodies. Results: The mean (± SD), 24-hour recovery determined, using anti-c, anti-C, anti-D, anti-Jk^a and anti-Fy^a as primary antibodies, was 78 ± 12%, the mean RBC survival time was 70.1 ± 26.5 days. Discussion: Flow cytometry using blood group antigen differences between donor and recipient appears to be suitable for the determination of RBC 24-hour recovery and survival time. This method requires no pretransfusional labeling of the RBCs, thus avoiding any bias by manipulation of the transfused RBCs. However, this method is applicable only to allogeneous transfusion.

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Viability and in vitro properties of AS‐1 red cells after gamma irradiation

Abstract: Irradiation has only a small effect on the properties of RBCs treated and stored according to the utilized protocols. Longer storage times after irradiation resulted in progressively reduced recovery while long-term survival remained unaffected.

Cited by 80 publications

References 18 publications

Irradiation eradication and pathogen reduction. Ceasing cesium irradiation of blood products

Irradiation eradication and pathogen reduction. Ceasing cesium irradiation of blood products

A systematic review of transfusion-associated graft-versus-host disease

Flow-Cytometric Determination of Survival Time and 24-Hour Recovery of Transfused Red Blood Cells

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