Evaluation of Streptozyme and Antistreptolysin O Tests in Streptococcal Pyodermal Nephritis

Bergner-Rabinowitz, S; Ofek, I.; Fleiderman, S.; Zohar, M.; Rabinowitz, K.; Ginsburg, Isaac

doi:10.1128/aem.26.1.56-58.1973

Cited by 9 publications

(4 citation statements)

References 5 publications

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“…Bei oberflächlichen Hautinfektionen durch A-Streptokokken (Pyodermien, Impetigo contagiosa) und den sich nicht selten daran anschließenden Glomerulonephritiden ist die Anti-DNase-B-Titer-Bestimmung (ADB-Test) dem ASO-Nachweis sogar überlegen (2,14,19,44,46,47,48 (9,18), nach anderen Berichten zeigte sich eine gute Empfindlichkeit des Streptozyme-Tests bei Streptokokkenerkrankungen (6,8). Die eigenen Untersuchungen verfolgten zwei Ziele:…”

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Antikörper gegen Streptococcus-pyogenes-Exoenzyme: Antistreptolysin O, Anti-DNase B und Streptozyme-Test

Lütticken¹,

Lw²,

Kluitmann³

et al. 1976

Dtsch med Wochenschr

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Sera from normal controls from patients with streptococcal diseases, and from other patients whose serum was sent for anti-streptolysin 0 determinations were tested for antistreptolysin 0 (ASO) and anti-DNase B (ADB) antibodies. The Streptozyme test was performed on the same sera. The upper limits of normal in the control population were established as 160 units for ASO and as 240 units for ADB, respectively. The usefulness of the ADB test in addition to the ASO test was confirmed: when both tests were performed elevated titers could be demonstrated in a higher percentage of various streptococcal diseases. With respect to streptococcal infections of the skin the anti-DNase B-test was superior to the ASO test. In this study of the Streptozyme test showed an elevated antibody titer in a lower proportion than the other two tests.

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Antikörper gegen Streptococcus-pyogenes-Exoenzyme: Antistreptolysin O, Anti-DNase B und Streptozyme-Test

Lütticken¹,

Lw²,

Kluitmann³

et al. 1976

Dtsch med Wochenschr

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“…Since ACF and STZ reagent share common antigens, some information as to the specificity of ACF antigens for beta-hemolytic streptococci may be inferred from results obtained using the STZ test. Studies in our own and other laboratories have shown that serum titers of A-STZ correlate well with those of the conventional antibodies, which are known to be specific for beta-hemolytic streptococcal infection (1,2,6,9,10). A-STZ antibodies are not stimulated in rabbits by immunization with S. pneumoniae or S. viridans.…”

Section: Discussionmentioning

confidence: 64%

Antigens of group A streptococci involved in passive hemagglutination reactions

Bisno¹,

Ofek²,

Beachey³

1976

Infect Immun

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Antibodies to streptococcal extracellular products were detected in rabbit sera by passive hemagglutination tests as early as 1 week after intravenous injection of live group A streptococci (strain C203S). Using these antibodies in immunoadsorbent columns, we prepared an antigenic fraction from crude concentrates of streptococcal extracellular products. The specific activity of this fraction in sensitizing glutaraldehyde-treated erythrocytes for passive hemagglutination tests and in absorbing passive hemagglutination antibodies from immune sera was increased by 80-fold and at least 24-fold, respectively, as compared with crude extracellular products. The fraction has been found to contain at least three serologically active antigens, which are of considerable interest because: (i) they gave rise to early and consistent immune responses both in humans and in experimental animals; (ii) they appear so far to be produced only by beta-hemolytic streptococci; and (iii) antibodies to these antigens are present in high titers in patients with acute rheumatic fever. Our data suggest that passive hemagglutination antigens may be distinct from those extracellular enzymes and hemolysins ordinarily employed in streptococcal serological studies.

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“…The streptozyme test employs a reagent that is a standard suspension of formaldehyde-treated sheep erythrocytes sensitized with group A streptococcal extracellular products (6). The information supplied by the producing firm and data obtained by others who have used the test (3,8,12) indicate that a positive streptozyme test will usually be observed with sera when one or more of the streptococcal antibody titers are: ASO, > 166 Todd units; ADN-B, >170; ANAD, >270; AH. .256; and ASK.…”

Section: Methodsmentioning

confidence: 99%

“…Others have compared the results obtained with the streptozyme, test in human sera to those obtained on the same sera with the serological tests for detecting ASO, AH, ASK, and ADN-B (3,8,12). Although each of these studies revealed a good correlation between the results with the streptozyme test and the conventional serological tests, there has been inadequate assessment of the possible frequency of false-positive and false-negative results with the streptozyme test.…”

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confidence: 99%

Specificity and Sensitivity of the Streptozyme Test for the Detection of Streptococcal Antibodies

Kholy¹,

Hafez²,

Krause³

1974

Applied Microbiology

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A comparison between the results of the streptozyme hemagglutination test and serological titers for anti-streptolysin 0 (ASO), anti-hyaluronidase (AH), anti-deoxyribonuclease B (ADN-B), and anti-nicotinamide adenine dinucleotidase (ANAD) was made in two groups of human sera. In one group, serological titers for all the four antibodies were lower than the threshold of sensitization reported by the producing firm. In the second group, the titer of at least one of the four antibodies was equal to or higher than the threshold. False-positive and false-negative reactions occur with those sera when one or more antibody titer is at or near the threshold of the test as described by the manufacturer. The test was positive for all sera where either the ASO was greater than 166 or the ANAD was greater than 270, and for 98% of the sera with ADN-B greater than 360. It is, therefore, concluded that the streptozyme test can be used as an adjunct to the clinical diagnosis of streptococcal infections and their nonsuppurative sequelae.

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Evaluation of Streptozyme and Antistreptolysin O Tests in Streptococcal Pyodermal Nephritis

Cited by 9 publications

References 5 publications

Antikörper gegen Streptococcus-pyogenes-Exoenzyme: Antistreptolysin O, Anti-DNase B und Streptozyme-Test

Antikörper gegen Streptococcus-pyogenes-Exoenzyme: Antistreptolysin O, Anti-DNase B und Streptozyme-Test

Antigens of group A streptococci involved in passive hemagglutination reactions

Specificity and Sensitivity of the Streptozyme Test for the Detection of Streptococcal Antibodies

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