Antikörper gegen Streptococcus-pyogenes-Exoenzyme: Antistreptolysin O, Anti-DNase B und Streptozyme-Test

Abstract

Sera from normal controls from patients with streptococcal diseases, and from other patients whose serum was sent for anti-streptolysin 0 determinations were tested for antistreptolysin 0 (ASO) and anti-DNase B (ADB) antibodies. The Streptozyme test was performed on the same sera. The upper limits of normal in the control population were established as 160 units for ASO and as 240 units for ADB, respectively. The usefulness of the ADB test in addition to the ASO test was confirmed: when both tests were performed elevated titers could be demonstrated in a higher percentage of various streptococcal diseases. With respect to streptococcal infections of the skin the anti-DNase B-test was superior to the ASO test. In this study of the Streptozyme test showed an elevated antibody titer in a lower proportion than the other two tests.

Detection of anti- DNase B antibody upper normal values in children's age groups who were admitted to hospital with noninfectious reasons

“…However in most of the studies, ULN of ADB has been determined as 200–240 IU/ml in compliance with our study results [4, 5, 6, 7]. In most of the studies upper limit of normal has been accepted as the value discriminating between the highest 20%, and the lowest 80% of the values after measurement of antibody titers.…”

“…In this study, ULN for ADB was indicated as 640 IU/ml. In a study performed by Lütticken et al in Germany, ULN value of ADB was found to be 240 IU/ml [4]. …”

Quantitative evaluation of variation in composition of the streptozyme agglutination reagent for detection of antibodies to group A streptococcal extracellular antigens

“…It is a relatively simple-to-perform slide agglutination test, and since its introduction in the early 1970s, it has become one of the most widely used tests in clinical and hospital laboratories for the determination of group A streptococcal antibodies. This agglutination test has been compared with the more conventional neutralization tests for streptococcal antibodies such as the anti-streptolysin O and the anti-deoxyribonuclease B tests (2,5,7,9); yet because the Streptozyme reagent contains multiple nonpurified streptococcal antigens coated onto ovine erythrocytes, the ability to standardize the reagent has been questioned (5,6,8). Preliminary work from this laboratory has suggested reagent variation from lot to lot (5,6).…”