Evaluation of six serological tests in diagnosis and postoperative control of pulmonary hydatid disease patients

Zarzosa, M.Pilar; Domingo, Antonio Orduña; Gutiérrez, Purificación; Alonso, Pedro L.; Cuervo, Milagros; Prado, Ana Prieto del; Bratos, Miguel Ángel; Garcı́a-Yuste, Mariano; Ramos, Guillermo; Torres, Antonio Rodrı́guez

doi:10.1016/s0732-8893(99)00079-6

Cited by 101 publications

(96 citation statements)

References 17 publications

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“…Although, ELISA was indicated as highly sensitive (100 %) in detecting anti-Echinococcus antibodies irrespective of the site of the cyst localization (Wattal et al, 1986), many authors reported ELISA not to be good enough to detect low antibody levels produced in pulmonary CE. Our ELISA results are similar to those obtained by Zarzosa et al (1999). In our WB, we could not get sensitivity or specificity results higher than 90 % for single bands, despite the high sensitivity and specificity results obtained with antigen 5 or antigen B by others (Kanwar et al, 1992;Sbihi et al, 1997).…”

Section: Discussionsupporting

confidence: 86%

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Comparative evaluation of Western Blotting in hepatic and pulmonary cystic echinococcosis

Akısü

Delıbaş

Biçmen³

et al. 2006

Parasite

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Summary :Many serological tests are widely used in the diagnosis of cystic echinococcosis (CE), caused by the larval stages of Echinococcus granulosus. The present study was carried for differentiation between hepatic and pulmonary cystic echinococcosis by Western Blotting (WB). A total of 121 sera from patients with hepatic CE (37), pulmonary CE (31) and controls (53; consisting of six healthy, seven Hymenolepis nana infection, 20 hepatic and 20 pulmonary diseases other than CE) were examined. In all of the CE patients, E. granulosus infection was confirmed by surgical intervention. Sera were previously tested using IHA and ELISA to detect the E. granulosus specific antibodies. Sera from hepatic cases of CE reacted with 16 polypeptides of 6-116 kDa and sera from pulmonary cases of CE reacted with 14 polypeptides of 4-130 kDa by Western Blotting. The WB test enabled the detection of antibodies in the hepatic CE samples for proteins of 24, 32-34, 44-46 and 52-54 kDa in molecular weight in 78.4 %, 75.7 %, 78.4 % and 89.2 % of the patients, respectively. In the pulmonary CE samples sera WB test enabled the detection of antibodies 24, 44-46, 100, 110, 116 and 120-124 kDa in molecular weight in 81.3 %, 75.0 %, 87.5 %, 71.9 %, 84.4 % and 65.6 % of the patients, respectively. We indicated that the antigenic components of high molecular weight can be good candidates for differentiation of hepatic CE from pulmonary CE. de 24,[32][33][34][44][45][46]4 %, 75,7 %, 78,4 % et 89,2 % des patients, respectivement, et, en cas de KH pulmonaire, de protéines de 24,[44][45][46] 100, 110,[116][117][118][119][120][121][122][123][124]3 %, 75,0 %, 87,5 %, 71,9 %, 84,4 % et 65,6 Résumé

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Section: Discussionsupporting

confidence: 86%

“…Pulmonary cysts usually yield low serologic response, whereas the hepatic cysts are usually accompanied by a sustained serological response (Zarzosa et al, 1999). In this study, the sensitivities of all tests performed for patients with pulmonary CE are lower than that performed for patients with hepatic CE.…”

Section: Discussionmentioning

confidence: 62%

Comparative evaluation of Western Blotting in hepatic and pulmonary cystic echinococcosis

Akısü

Delıbaş

Biçmen³

et al. 2006

Parasite

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“…Diagnosis of hydatid disease is established by imaging methods: radiography, echosonography, computerized tomography (CT), magnetic resonance (MR), by serologic tests: indirect fluorescency of antibodies (IF), immunoenzymatic tests (ELISA), and microscopy of cysts punctate searching for protoscolices and echinococcal particles. (Zarzosa et al, 1999). Immunoenzymatic tests (ELISA), diagnostic kits were used: Nova Lisa, Echinococcus-IgG ELISA, Nova Tec, Immunodiagnostica Gmbh).…”

Section: Introductionmentioning

confidence: 99%

“…After rinsing with 100 µl with Washing Solution three times 100 µl of tetramethylbenzidine (TMB) Substrat Solution was applied and incubated for 15 minutes in dark at room temperature. A Stop Solution (100 µl) was then instilled in all wells and results read in 30 minutes on spectrophotometer with 450 nm filter (Zarzosa et al, 1999). Patients were treated with albendazole 15 mg/kg/day for 28 days before percutaneous aspiration of the cyst.…”

Section: Introductionmentioning

confidence: 99%

Diagnostics of Echinococcus granulosus particles in hepatic cysts punctate of seropositive patients

2014

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SummaryDiagnosis of liver hydatid cyst disease was established by ultrasound and CT examination in 76 patients and confirmed with positive IF and ELISA tests. We found them elective for percutaneous punction after albendazole treatment. Obtained specimens were examined for presence of echinococcal particles and 48 were positive (63.16 %) and it reflects the specifity of applied serological tests. Development of more specific serological tests combined with existent radiological methods will contribute to more accurate diagnostic approach but analysis of cyst specimens will remain the cornerstone of exact diagnosis.

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“…Nevertheless, serology is more specific but less sensitive than imagery [6]. Among the serologic tests, IgG ELISA and indirect hemaglutination (IHA) are 2 important tests often used in diagnosis and in monitoring the disease in the postoperative period [7][8][9]. In this study, 4 different serologic tests were applied to the sera of a group of patients with pulmonary hydatid disease, and the sensitivity, the specificity, positive and negative predictive values of the tests were assessed.…”

mentioning

confidence: 99%

Evaluation of Two ELISA and Two Indirect Hemagglutination Tests for Serodiagnosis of Pulmonary Hydatid Disease

Eri̇ş¹,

Akısü

Aksoy

2009

Korean J Parasitol

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Human primary hydatid is most frequently seen in the liver and the lung. Radiology and serology should be assessed together to establish a definite diagnosis for pulmonary hydatid disease [1,2]. There are studies reporting that the antibody response is lower in lung hydatid cysts compared with liver hydatid cysts; and serologic tests have 80-90% sensitivity in liver hydatid cysts and 50-56% in pulmonary hydatid cysts [3][4][5]. The best result in the diagnosis of pulmonary hydatid cysts is obtained by collective use of radiology and serology. Nevertheless, serology is more specific but less sensitive than imagery [6]. Among the serologic tests, IgG ELISA and indirect hemaglutination (IHA) are 2 important tests often used in diagnosis and in monitoring the disease in the postoperative period [7][8][9]. In this study, 4 different serologic tests were applied to the sera of a group of patients with pulmonary hydatid disease, and the sensitivity, the specificity, positive and negative predictive values of the tests were assessed.The operation results were taken as the golden standard, and the sensitivity and the specificity of the tests were calculated accordingly [2,9-11]. Patients' sera were separated so as to perform 4 tests. All sera were frozen and kept -80℃ in aliquots until use.Two ELISA and 2 IHA tests were performed for all patient sera. ELISA-lab and IHA-lab were prepared in our laboratory using hydatid cystic fluid (HCF) from fertile cysts of sheep liver. The antigen was prepared with a pool of HCF centrifuged at 15,000 g for 30 min. The supernatant was used as crude antigen. The commercial Hydatidosis IgG ELISA (Vircell SL, Granada, Spain) test was employed according to the manufacturer's instructions. Each test reaction was read spectrophotometrically at 405 nm. Cut-off optical density was calculated in cut-off control serum for IgG. Antibody index (AI) was calculated as follows: AI = sample OD/cut-off OD × 10. AI greater than 11 was accepted as seropositive, and values lower than 9 were considered as seronegative. Values between 9 and 11 were accepted as borderline and the test was repeated for all these sera. ELISA was prepared by the method of Coltorti et al. [12]. The readings were taken at 410 nm. The serum dilutions that resulted in an absorbance at least twice the mean absorbance of wells containing negative control samples were considered positive. Thus, 1 : 160 and higher dilutions was accepted as positive [11]. The commercial Hydatidosis IHA (Hydatidose, Fumouze Laboratoires, France) test was employed according to the manufacturer's in- Evaluation of Two ELISA and Two Indirect Hemagglutination Tests for Serodiagnosis of Pulmonary Hydatid DiseaseKorean J Parasitol. Vol. 47, No. 4: 427-429, December 2009 DOI: 10.3347/kjp.2009 427 Abstract: To establish a definite diagnosis for pulmonary hydatid disease, combination of radiology and serology is useful. In this study, 19 preoperative sera from patients with surgically confirmed pulmonary hydatidosis, 40 sera from patients with other parasitosis an...

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Evaluation of six serological tests in diagnosis and postoperative control of pulmonary hydatid disease patients

Cited by 101 publications

References 17 publications

Comparative evaluation of Western Blotting in hepatic and pulmonary cystic echinococcosis

Comparative evaluation of Western Blotting in hepatic and pulmonary cystic echinococcosis

Diagnostics of Echinococcus granulosus particles in hepatic cysts punctate of seropositive patients

Evaluation of Two ELISA and Two Indirect Hemagglutination Tests for Serodiagnosis of Pulmonary Hydatid Disease

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