Evaluation of several ionic liquids for in situ hydrolysis of empty fruit bunches by locally-produced cellulase

Elgharbawy, Amal A.M.; Alam, Md. Zahangir; Kabbashi, Nassereldeen Ahmed; Moniruzzaman, Muhammad; Jamal, Parveen

doi:10.1007/s13205-016-0440-8

Cited by 18 publications

(16 citation statements)

References 50 publications

(49 reference statements)

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“…Figure 3(a) shows the compact and fibrillated structure which becomes loosened after the detachment of microfibrils form one another following the sequential pretreatments. The disordered structure increased the surface area and the porosity leading to the increased accessibility of enzymes [42] which is in agreement with the results of observed with scanning electron microscopy [43]. Fang et al showed that the deformed structure and exposed fibres of corn stover after undergoing the thermo-chemical pretreatment using SEM [42].…”

Section: Scanning Electron Microscopysupporting

confidence: 80%

Multi-Scale Structural Studies of Sequential Ionic Liquids and Alkali Pretreated Corn Stover and Sugarcane Bagasse

Kaur¹,

Sahni²

2018

GSC

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The complexity of plant cell walls impedes the conversion of cellulosic biomass to sugars. Pretreatment becomes a necessity to increase the digestibility of biomass. An in-depth understanding of the structure and underlying mechanisms governing deconstruction process is important. In the present study, the comprehensive investigation of morphological and structural changes in corn stover and sugarcane bagasse following ionic liquids dissolution and alkaline extraction was done using Fourier transform infrared spectroscopy, Thermogravimetric analysis, Confocal scanning laser microscopy, Atomic force microscopy and Dynamic light scattering studies. Both the substrates were pretreated with ILs 1-ethyl-3-methylimidazolium acetate and 1-butyl-3-methylimidazolium acetate followed by alkaline extraction. The pronounced changes such as lignin, hemicelluloses removal and decreased cellulose crystallinity after the pretreatments lead to the structural transformation of matrix polymers. The enzymatic hydrolysis showed 90% theoretical sugar yield in case of sugarcane bagasse and 80% in corn stover following synergistically combined pretreatments.

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Section: Scanning Electron Microscopysupporting

confidence: 80%

Multi-Scale Structural Studies of Sequential Ionic Liquids and Alkali Pretreated Corn Stover and Sugarcane Bagasse

Kaur¹,

Sahni²

2018

GSC

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“…The highest sugar production was recorded with [Cho] OAc in the previous research by the authors (Elgharbawy et al 2016a). As acquired from the analysis, the specimen treated with [Cho]OAc contained 24, 22.5, and 0.09 g/L of glucose, xylose, and arabinose individually.…”

Section: Pretreatment and Saccharification Of Efbmentioning

confidence: 60%

“…Locally produced cellulase was extracted as a product of the fermentation of PKC as previously described in our report (Elgharbawy et al 2016a). The cellulase enzyme was called 'PKC-Cel' in reference to the main medium that was used for production.…”

Section: Production Of Pkc-celmentioning

confidence: 99%

Chemical and structural changes of pretreated empty fruit bunch (EFB) in ionic liquid-cellulase compatible system for fermentability to bioethanol

et al. 2018

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The pretreatment of empty fruit bunch (EFB) was conducted using an integrated system of IL and cellulases (IL-E), with simultaneous fermentation in one vessel. The cellulase mixture () was derived from by solid-state fermentation. Choline acetate [Cho]OAc was utilized for the pretreatment due to its biocompatibility and biodegradability. The treated EFB and its hydrolysate were characterized by the Fourier transform infrared spectroscopy, scanning electron microscopy, and chemical analysis. The results showed that there were significant structural changes in EFB after the treatment in IL-E system. The sugar yield after enzymatic hydrolysis by the was increased from 0.058 g/g of EFB in the crude sample (untreated) to 0.283 and 0.62 ± 06 g/g in IL-E system after 24 and 48 h of treatment, respectively. The EFB hydrolysate showed the eligibility for ethanol production without any supplements where ethanol yield was 0.275 g ethanol/g EFB in the presence of the IL, while lower yield obtained without IL-pretreatment. Moreover, it was demonstrated that furfural and phenolic compounds were not at the level of suppressing the fermentation process.

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“…The presence of IL usually causes enzyme deactivation due to denaturalization by the interaction of the IL anion with hydrogen bonds in the enzyme proteins. ,, Enzymatic hydrolysis yield is less affected by the volume of water employed in wood pretreated with [Emim][OAc] than it is in wood pretreated with [Ch][OAc]. Samples treated with [Emim][OAc] present the same digestibility when washing with 3.5, 5.5, and 20 g water/g IL (from Figure , IL content in biomass of 4.12, 1.21, and 0.00 g IL/g biomass, respectively), and their difference with the sample washed with 2.5 g water/g IL (7.95 g IL/g biomass) is approximately 10%.…”

Section: Resultsmentioning

confidence: 99%

Technoeconomic Assessment of a Biomass Pretreatment + Ionic Liquid Recovery Process with Aprotic and Choline Derived Ionic Liquids

Ovejero-Pérez

Ayuso

Rigual

et al. 2021

ACS Sustainable Chem. Eng.

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Ionic liquids have shown promising results in biomass pretreatment; however, an extensive water washing step is necessary. This fact increases not only the processing costs but also those associated with the ionic liquid recovery step. In this work, Eucalyptus globulus wood pretreated with two acetate-based ionic liquids, namely 1-ethyl-3-methylimidazolium acetate and choline acetate, has been washed with increasing volumes of water in order to analyze the influence of the amount of washing water used on the subsequent enzymatic hydrolysis stage and on the IL recovery costs. Vapor−liquid equilibria data of recovered ionic liquid/water mixtures have been determined to simulate the IL recovery step with Aspen Plus, calculating the operating costs using the Aspen Plus Economics Analyzer afterward. [Emim][OAc] was more efficiently washed and more effective toward wood pretreatment than [Ch][OAc]. Both IL/water systems were successfully modeled, and simulation studies showed that incremental volumes of water led to higher operating costs in the IL recovery step that are compensated with less IL makeup costs. Therefore, this work offers a technoeconomical analysis of the IL recovery step in a real biorefinery pretreatment process as a function of the volume of water used in the pretreated wood washing stage.

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Evaluation of several ionic liquids for in situ hydrolysis of empty fruit bunches by locally-produced cellulase

Cited by 18 publications

References 50 publications

Multi-Scale Structural Studies of Sequential Ionic Liquids and Alkali Pretreated Corn Stover and Sugarcane Bagasse

Multi-Scale Structural Studies of Sequential Ionic Liquids and Alkali Pretreated Corn Stover and Sugarcane Bagasse

Chemical and structural changes of pretreated empty fruit bunch (EFB) in ionic liquid-cellulase compatible system for fermentability to bioethanol

Technoeconomic Assessment of a Biomass Pretreatment + Ionic Liquid Recovery Process with Aprotic and Choline Derived Ionic Liquids

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