Evaluation of separation properties of stationary phases in supercritical fluid chromatography; deazapurine nucleosides case study

Kozlov, Oleksandr; Kadlecová, Zuzana; Tesařová, Eva; Kalíková, Květa

doi:10.1016/j.microc.2019.104137

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…Due to this finding, the target analytes in SFC were notably expanded from hydrophobic to hydrophilic compounds including amino acids, 17,66,67) peptides, 66,68,69) chiral compounds, [70][71][72] and achiral compounds. 73) In addition to water, a small amount of diethylamine, triethylamine, or trifluoroacetic acid (TFA) added to a modifier also improved the efficiency, retention time and resolutions of biological molecules including nucleosides, 74) peptides, 68,69,75,76) and proteins. 77,78) As a next step, SFC purifications of human bradykinin, bovine insulin, bovine ubiquitin, bovine cytochrome c, and equine apomyoglobin were demonstrated on a preparative scale.…”

Section: Microfluidic Technology For Sfcmentioning

confidence: 99%

Emerging Separation Techniques in Supercritical Fluid Chromatography

Yamamoto

Machida

Kotani

et al. 2021

CHEMICAL & PHARMACEUTICAL BULLETIN

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Supercritical fluid chromatography (SFC) has unique separative characteristics distinguished from those of HPLC and gas chromatography. At present, SFC is widely used and there are many applications in various biological, medical, and pharmaceutical fields. In this review, we focus on recently developed novel techniques related to SFC separation including: new column stationary phases, microfluidics, twodimensional separation, and gas-liquid separation. In addition, we discuss the application of SFC using a water-containing modifier to biological molecules such as amino acids, peptides, and small proteins that had been challenging analytes.

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Section: Microfluidic Technology For Sfcmentioning

confidence: 99%

Emerging Separation Techniques in Supercritical Fluid Chromatography

Yamamoto

Machida

Kotani

et al. 2021

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…Alcohols such as methanol (MeOH), ethanol (EtOH), and propan‐2‐ol are the most common modifiers (co‐solvents) in sub/supercritical MPs 67–70 . The nature and portion of co‐solvent in MP may affect not only retention, but also enantioselectivity and enantiomer elution order 71,72 .…”

Section: Theoretical Studies Of Macrocyclic Glycopeptides In Sfcmentioning

confidence: 99%

“…Slight decrease in enantioresolution (on average 6%) was observed when increasing back pressure from 100 to 200 bar especially due to the retention and efficiency decrease. Higher back pressure usually results in decrease of retention and efficiency accompanied by worsening of resolution compared to lower back pressure 96,97,70 …”

Section: Theoretical Studies Of Macrocyclic Glycopeptides In Sfcmentioning

confidence: 99%

“…Alcohols such as methanol (MeOH), ethanol (EtOH), and propan‐2‐ol are the most common modifiers (co‐solvents) in sub/supercritical MPs. 67 , 68 , 69 , 70 The nature and portion of co‐solvent in MP may affect not only retention, but also enantioselectivity and enantiomer elution order. 71 , 72 Methanol (with or without additives) is the most often used co‐solvent to increase the elution strength of the MP with MG‐based CSPs in SFC.…”

Section: Theoretical Studies Of Macrocyclic Glycopeptides In Sfcmentioning

confidence: 99%

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Macrocyclic glycopeptide‐based chiral selectors for enantioseparation in sub/supercritical fluid chromatography

Folprechtová

Kalíková

2020

Analytical Science Advances

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Increasing number of reported works dealing with macrocyclic glycopeptide‐based columns in sub/supercritical fluid chromatography (SFC) points to the growing interest in this area. With the development and production of sub 2 µm fully porous particles and superficially porous particles with bonded macrocyclic glycopeptides, significant improvements have been made in ultrafast high efficiency chiral SFC. This review article gives an overview of macrocyclic glycopeptide‐based chiral selectors that were used in theoretical studies and/or applications in SFC. The review covers the period from 1997 when macrocyclic glycopeptides were first used in SFC till the end of July 2020 according to Web of Science. This work can also be helpful to analysts searching for an appropriate method for the separation/determination of enantiomers of their interest.

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“…A diverse analytical approach has been developed for the analysis of nucleosides and nucleotides. There are methods for the identification and separation of nucleosides from biomaterials, which include liquid chromatography–mass spectrometry [4], high supercritical fluid chromatography [5], high‐performance liquid chromatography [6], and capillary electrophoresis [7]. The recently employed new separation technique for nucleosides is the molecularly imprinted polymer‐based magnetic nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of molecularly imprinted magnetic nanoparticles for selective cytidine adsorption

Öngün

Akgönüllü

Yavuz

et al. 2020

Separation Science Plus

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Magnetic molecularly imprinted nanoparticles were fabricated for cytidine adsorption. Molecularly imprinted polymers were prepared by complexing N‐methacryloyl‐l‐histidine/Cu(II) and cytidine as a template molecule in a highly cross‐linked polymer. Magnetic molecularly imprinted nanoparticles were tested in batch experiments with aqueous samples of cytidine, and several parameters were investigated. The N‐methacryloyl‐l‐histidine/Cu(II) complex was selected to obtain a rebinding of cytidine on the magnetic nanoparticles. The template molecule was removed using 1.0 M NaCl as a desorption agent. The adsorption of cytidine was optimal in a buffer solution of pH 7.4 for 120 min with adsorption capacity of 39.77 mg/g. The imprinted polymer‐based magnetic nanoparticles were selective toward cytidine as compared to the analogs (uridine and thymidine). The adsorption fitted a Langmuir isotherm model with an R2 value of 0.9861 and the Qmax value of 43.86 mg/g for the magnetic molecularly imprinted nanoparticles.

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Evaluation of separation properties of stationary phases in supercritical fluid chromatography; deazapurine nucleosides case study

Cited by 7 publications

References 34 publications

Emerging Separation Techniques in Supercritical Fluid Chromatography

Emerging Separation Techniques in Supercritical Fluid Chromatography

Macrocyclic glycopeptide‐based chiral selectors for enantioseparation in sub/supercritical fluid chromatography

Synthesis of molecularly imprinted magnetic nanoparticles for selective cytidine adsorption

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