Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. cell suspension culture under magnetite nanoparticles and static magnetic field elicitation

Taghizadeh, Marzieh; Nasibi, Fatemeh; Kalantari, Khosrow Manouchehri; Ghanati, Faezeh

doi:10.1007/s11240-018-01530-1

Cited by 44 publications

(16 citation statements)

References 37 publications

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“…ROS may act as a normal signal for adaptation to Cu stress and could induce the accumulation of TPC in lentil roots [49]. Elicitation of magnetite NPs and static magnetic field elicitation can increase the content of phenolic compounds and polyphenol oxidase in the CSC of Dracocephalum polychaetum [9]. TPC and TFC can also be determined by both the surface area and size of the NPs used.…”

Section: Tpc and Tfc In Cscmentioning

confidence: 99%

Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

et al. 2019

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Gymnema sylvestre is a plant that is enriched in bioactive compounds. In particular, gymnemic acids (GA) and phenolic compounds (PC) are pharmaceutically important. There is a commercial demand for naturally occurring bioactive compounds, but their availability is limited due to geographical and seasonal variations. The elicitation approach can enhance the biosynthesis of phytochemicals during in vitro culture of G. sylvestre. Here, to further improve gymnemic acid II (GA II) and phenolic compounds (PC) production by G. sylvestre, cell suspension cultures (CSC), which has attracted attention for the production of essential phytochemicals, was explored using copper oxide nanoparticles (CuO NPs). Callus was obtained on MS medium containing 2,4-dichlorophenoxyacetic acid, kinetin, phytoagar, and sucrose. Agar-free MS medium was used to initiate CSC, which was treated with three concentrations of CuO NPs (1, 3 or 5 mg/L). Treatment for 48 h with 3 mg/L CuO NPs resulted in the greatest yields of GA II, total phenolics, and flavonoids. The cultures also displayed pronounced antioxidant, antidiabetic, anti-inflammatory, antibacterial, antifungal, and anticancer activities. The use of CuO NPs (3 mg/L) significantly increased the production of GA II (nine-fold) and PC compared to unamended CSC. We propose that CSC and use of nanoparticles (NPs) as a new generation of elicitors, offer a suitable prospect for the production of bioactive compounds.

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Section: Tpc and Tfc In Cscmentioning

confidence: 99%

Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

et al. 2019

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“…Moreover, a higher concentration of FeNPs (135 μg/L) induced stress, which produced a higher TFC [79]. Recently, magnetic Fe2O3NPs in the presence of a static magnetic field (SMF) resulted in the enhanced production of rosmarinic acid, naringin, apigenin, thymol, carvacrol, quercetin and rutin, TPC, TFC, anthocyanins, lignin and malondialdehyde in a cell suspension culture of D. polychaetum [80]. The use of magnetic fields has recently been proposed for plant growth and the production of plant secondary metabolites since they are less hazardous for the environment [81].…”

Section: Various Metallic Oxide Nps As An Elicitor Of Flavonoidsmentioning

confidence: 99%

Nano-Elicitation as an Effective and Emerging Strategy for In Vitro Production of Industrially Important Flavonoids

et al. 2021

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Flavonoids represent a popular class of industrially important bioactive compounds. They possess valuable health-benefiting and disease preventing properties, and therefore they are an important component of the pharmaceutical, nutraceutical, cosmetical and medicinal industries. Moreover, flavonoids possess significant antiallergic, antihepatotoxic, anti-inflammatory, antioxidant, antitumor, antiviral, and antibacterial as well as cardio-protective activities. Due to these properties, there is a rise in global demand for flavonoids, forming a significant part of the world market. However, obtaining flavonoids directly from plants has some limitations, such as low quantity, poor extraction, over-exploitation, time consuming process and loss of flora. Henceforth, there is a shift towards the in vitro production of flavonoids using the plant tissue culture technique to achieve better yields in less time. In order to achieve the productivity of flavonoids at an industrially competitive level, elicitation is a useful tool. The elicitation of in vitro cultures induces stressful conditions to plants, activates the plant defense system and enhances the accumulation of secondary metabolites in higher quantities. In this regard, nanoparticles (NPs) have emerged as novel and effective elicitors for enhancing the in vitro production of industrially important flavonoids. Different classes of NPs, including metallic NPs (silver and copper), metallic oxide NPs (copper oxide, iron oxide, zinc oxide, silicon dioxide) and carbon nanotubes, are widely reported as nano-elicitors of flavonoids discussed herein. Lastly, the mechanisms of NPs as well as knowledge gaps in the area of the nano-elicitation of flavonoids have been highlighted in this review.

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“…In the exponential growth stage, we exposed the cells to chitosan at the varying concentrations (0, 50, 100, and 200 mg L − 1 ), which was performed from day 8 to 12 following sub-cultures (T1) and day 12 to 16 following sub-cultures (T2). Some empirical studies and reviewed literature were used as the basis for selection of the elicitation period and concentrations (Chong et al 2005;Khan et al 2003;Taghizadeh et al 2019) When elicitations were nished, we harvested the cells from the suspension cultures by ltration. For this purpose, a Buchner funnel was utilized with a nylon mesh in frozen and vacuum state in liquid N 2 , and it was maintained at -80°C for subsequent biochemical surveys.…”

Section: Elicitor Treatmentmentioning

confidence: 99%

“…The observed differences in the values of MDA at 50 ppm chitosan were not signi cant probably due to the supplementary impacts of higher accumulations of phenolics at chitosan elicitation via ROS detoxi cation and membrane integrity protection. The effectiveness of secondary metabolites (e.g., phenolics) in the deceleration of lipid peroxidation is attributed to their free radical-scavenging ability (Taghizadeh et al 2019). The lipid peroxidation might also mediate signal transduction resulting in the increased generation of secondary metabolites.…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Enhancement Production of Phenolic Compounds in The Cell Suspension Culture of Iberis Amara L.: The Effect of Chitosan Elicitation

Taghizadeh

Nekonan²,

Setorki

2021

Preprint

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Iberis amara L. medicinal herb is well-known for having pharmacological values although its use has been challenged by the low levels of secondary metabolites. To address this issue, this study focused on evaluating the effect of explant, photoperiod, and plant growth regulators (PGRs) to find the optimum medium for inducing callus and establishing cell suspension in I. amara, followed by investigating the chitosan effect on some secondary metabolites. From our observations, the optimum condition for induced callus was achieved from the leaf explants in Murashige and Skoog (MS) media completed with 3 mg L− 1 6-benzylaminopurine(BAP) and 1 mgL− 1 1-naphthalene acetic acid(NAA) under 16-h light/8-h dark photoperiod. The MS enhanced with 3 mgL− 1BAP, 1 mg L− 1NAA, and 2% (w/v) sucrose appeared to be optimum conditions for suspension establishment. Thus, the cells were exposed to different concentrations of chitosan (200, 100, 50, and 0 mg L− 1) in their exponential growth stage from day 8 to 12 and day 12 to 16 following sub-cultures (T1) and sub-cultures (T2), respectively. The results showed that the 50 mgL− 1 chitosan significantly improved the total phenol, flavonoid, flavonol, and anthocyanin content in the I. amara in a dose-dependent manner. The highest malondialdehyde (MDA) amount, as a result of lipid peroxidation, was observed under the 200 ppm chitosan elicitation. Overall, these novel findings demonstrated the possibility of applying the cell suspension of I. amara treated with chitosan as a helpful approach for improving synthesizing phenolic compounds under controlled and sterile conditions without genetic modifications in medicinal herbs.

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Evaluation of secondary metabolites and antioxidant activity in Dracocephalum polychaetum Bornm. cell suspension culture under magnetite nanoparticles and static magnetic field elicitation

Cited by 44 publications

References 37 publications

Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

Nano-Elicitation as an Effective and Emerging Strategy for In Vitro Production of Industrially Important Flavonoids

Enhancement Production of Phenolic Compounds in The Cell Suspension Culture of Iberis Amara L.: The Effect of Chitosan Elicitation

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