Evaluation of Salivary Vitamin C and Catalase in HIV Positive and Healthy HIV Negative Control Group

Ahmadi‐Motamayel, Fatemeh; Vaziri-Amjad, Samaneh; Goodarzi, Mohammad Taghi; Poorolajal, Jalal

doi:10.2174/1871526517666170116142547

Cited by 7 publications

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“…From the results, they concluded that salivary antioxidant capacity could be altered by HIV. 18 Nosratzehi et al 15 assessed and compared the cotinine levels in the saliva of hookah smokers, nonsmokers, and passive smokers. They analyzed a total of 150 subjects, of which 50 were passive smokers, 50 were nonsmokers, and remaining 50 were hookah smokers.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Salivary Catalase, α-Amylase, and Cotinine Levels in Chronic Smokers: A Comparative Study

Singh

Sharma

Rohilla³

et al. 2018

The Journal of Contemporary Dental Practice

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Introduction:One of the common practices observed in many parts of the world is smoking, of which tobacco forms an important constituent which is burned and inhaled. Smoking is known to have potential effect on body's immune system, antioxidants level, and salivary cotinine levels. Hence, we planned the present study to evaluate the impact of cigarette smoke on salivary antioxidant levels and cotinine levels in smokers and nonsmokers. Materials and methods:The present study included assessment of salivary parameters of smokers and nonsmokers. A total of 400 subjects were analyzed, of which 200 were active smokers and 200 were nonsmokers. Unstimulated salivary samples were taken and assessment of α-amylase levels was done using biochemical kit and spectrophotometer. Assessment of salivary catalase (CAT) activity was done using Luck method. For the determination of cotinine levels, Bioassay Technology Laboratory kit was used using enzyme-linked immunosorbent assay (ELISA) technique. After the assessment of levels of all the salivary parameters, all the data were recorded, compiled, and analyzed.Results: α-Amylase in smokers and nonsmokers group was found to be 206.25 and 169.85 U/mL respectively. Nonsignificant results were obtained while comparing the salivary α-amylase levels among the two study groups. Nonsignificant results were Assessment of Salivary

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Section: Discussionmentioning

confidence: 99%

Assessment of Salivary Catalase, α-Amylase, and Cotinine Levels in Chronic Smokers: A Comparative Study

Singh

Sharma

Rohilla³

et al. 2018

The Journal of Contemporary Dental Practice

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“…It can increase in hypoxia due to the appearance of oxidant metabolites and there is evidence that salivary UA is altered in diseases such as oral lichen planus or diabetes ( 13 , 16 ). Catalase is an enzyme capable of removing ROS from saliva and its activity is altered in patients with different diseases such as human immunodeficiency virus ( 17 ). Components of the oxidant system can also be measured in saliva, namely the advanced oxidation protein products (AOPP) and hydrogen peroxide (H 2 O 2 ).…”

Section: Introductionmentioning

confidence: 99%

Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

Barranco

Rubio

Tvarijonaviciute

et al. 2018

Biochem. med. (Online)

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IntroductionIn this report, we aimed to examine the stability of various analytes in saliva under different storage conditions.Materials and methodsAlpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at – 20 ºC and – 80 ºC.ResultsAt RT, changes appeared at 6 hours for TEA and H2O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H2O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At – 20 ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2O2. At – 80 ºC observed changes were after 3 months for TEA and H2O2.ConclusionsIn short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more stable at - 80 ºC than at – 20 ºC.

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The “exposure-based cross-sectional” study design: a novel observational study design applicable to rare exposures

Poorolajal

2022

Biostatistics & Epidemiology

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Evaluation of Salivary Vitamin C and Catalase in HIV Positive and Healthy HIV Negative Control Group

Abstract: HIV can alter salivary antioxidant capacity as well as vitamin C and catalase levels. Saliva may reflect serum antioxidative changes in these patients. Therefore, further research is necessary on salivary and serum oxidants and the antioxidant changes.

Cited by 7 publications

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Assessment of Salivary Catalase, α-Amylase, and Cotinine Levels in Chronic Smokers: A Comparative Study

Assessment of Salivary Catalase, α-Amylase, and Cotinine Levels in Chronic Smokers: A Comparative Study

Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

The “exposure-based cross-sectional” study design: a novel observational study design applicable to rare exposures

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