Evaluation of root-to-shoot de novo organogenesis in wild guava species, Psidium schenckianum and P. guineense (Myrtaceae)

Santos, Márcia Adriana Carvalho dos; Rêgo, Maílson Monteiro do; Queiróz, M. A. de; Cruz, Ana Cláudia Ferreira da; Silva, Thaís Cristina Ribeiro da; Pinheiro, Marcos Vinícius Marques; Batista, Diego Silva; Rocha, Diego Ismael; Otoni, Wagner Campos

doi:10.1007/s42535-020-00177-8

Cited by 1 publication

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References 48 publications

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“…Em espécies da família Myrtaceae nativas do Brasil, estudos de cultivo in vitro vêm sendo conduzidos com o objetivo de entender o comportamento de cada espécie quando dispostas aos mais diversos componentes do meio de cultura e aprimorar o uso da técnica. São encontrados na literatura relatos promissores do uso da cultura de tecidos para essas espécies, como, por exemplo, a indução de embriogênese somática em camucamuzeiro (Myrciaria dubia (Kunth) McVaugh) (ARAÚJO et al, 2015); jaboticabeira (Plinia peruviana (Poir) Govaerts) (SILVEIRA et al, 2020); pitangueira (Eugenia uniflora L.) (STEFENON et al, 2020); e goiabeira serrana (STEFANELLO et al, 2005;CANGAHUALA-INOCENTE et al, 2007;FRAGA et al, 2012); de micropropagação de explantes provenientes de plantas adultas em pitangueira (Eugenia uniflora L.) (FREIRE et al, 2018); camucamuzeiro (Myrciaria dubia (Kunth) McVaugh) (ARAUJO et al, 2016); e araçazeiro (Psidium cattleyanum) ) e de multiplicação por explantes provenientes de plântulas germinadas in vitro em guabijuzeiro (Myrcianthes pungens O. Berg) D. Legrand (SOUZA et al, 2011;SOUZA et al, 2020); pitangueira (Eugenia uniflora L.) (SILVA et al, 2014); araçazeiro do campo (Psidium schenckianum Kiaersk) e araçazeiro verdadeiro (Psidium guineense Swartz) (SANTOS et al, 2021); casaqueira (Campomanesia rufa (O. Berg) Nied) ; gabirobeira (Campomanesia adamantium (Cambess) O. Berg) (ROSSATO et al, 2019); guabirobeira (Campomanesia xanthocarpa O. Berg) (MACHADO et al, 2020); e cambucizeiro (Myrciaria tenella O. Berg.)…”

Section: Introductionunclassified

Cultivo >i<in vitro>/i< de cerejeira-do-Rio-Grande (>i<Eugenia involucrata>/i< DC.) e de cambucizeiro (>i<Campomanesia phaea>/i< (O. Berg) Landrum)

Júnior¹,

de²

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In vitro cultivation of cerejeira-do-Rio-Grande (Eugenia involucrata DC.) and cambucizeiro (Campomanesia phaea (O. Berg) Landrum) Brazilian biodiversity is one of the richest in the world with different biomes that comprise its flora and fruit species with great commercial exploitation value. The Atlantic Forest, the third-largest biome in the country, is considered a global hotspot for conservation and biological diversity, with numerous native fruits, including species of the Myrtaceae family, such as the cerejeira-do-Rio-Grande (Eugenia involucrata DC.) and the cambucizeiro (Campomanesia phaea (O. Berg) Landrum). These species have great potential for commercial use, in addition to being extremely important for reforestation and urban afforestation. The cerejeira-do-Rio-Grande and cambucizeiro fruits have high nutritional value, being commercialized fresh or processed, contributing to the diversification of production of Brazilian fruit growers. The commercial multiplication of these fruit trees is carried out through the seminal route and is hampered due to the low storage potential of the seeds. Conventional methods of asexual multiplication in both fruit trees are inefficient, and scientific studies willing to optimize them are scarce. Micropropagation, multiplication of plants through in vitro culture techniques, is an alternative method for large-scale production of commercial seedlings of these fruit trees. Therefore, this work aimed to establish protocols of germination and in vitro multiplication of cerejeira-do-Rio-Grande using nodal explants from seedlings germinated in aseptic conditions and to evaluate the different processes and culture medium components for the establishment and multiplication of nodal and leaf explants of cerejeira-do-Rio-Grande and cambucizeiro, originated from plants grown in the greenhouse. The best conditions for seed germination and seedling development of cerejeira-do-Rio-Grande are when they are cultivated in an MS medium supplemented with 15 g L -1 of sucrose, removing the tegument and cultivating in the dark; for in shoot induction and development, the use of 2.5 mg L -1 of 6benzylaminopurine (BAP) and JADS culture medium is recommended; in the subculture, the best results were obtained when adding 1.0 to 2.5 mg L -1 of BAP; finally, it is possible to obtain high rates of rooting of shoots when using WPM, ½ WPM, ¼ WPM, and JADS culture media without any growth regulators. The seedling acclimatization is achieved by using a commercial potting mix (based on pine bark, peat, and vermiculite) mixed with vermiculite in a 1:1 ratio. For the in vitro culture of cerejeira-do-Rio-Grande, explants from plants kept in the greenhouse, the lowest contamination rate, oxidation, and greater survival rate of nodal explants, the collection must be carried out in the autumn, performing the asepsis with sodium hypochlorite (NaClO) containing 1.25% of active chloride. The addition of 1.0 mg L -1 of BAP in the presence of 0.1 mg L -1 of ANA (naphthalene acetic acid) and 2.5 mg L -1 of BAP e...

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Section: Introductionunclassified

Cultivo >i<in vitro>/i< de cerejeira-do-Rio-Grande (>i<Eugenia involucrata>/i< DC.) e de cambucizeiro (>i<Campomanesia phaea>/i< (O. Berg) Landrum)

Júnior¹,

de²

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show abstract

Evaluation of root-to-shoot de novo organogenesis in wild guava species, Psidium schenckianum and P. guineense (Myrtaceae)

Cited by 1 publication

References 48 publications

Cultivo >i<in vitro>/i< de cerejeira-do-Rio-Grande (>i<Eugenia involucrata>/i< DC.) e de cambucizeiro (>i<Campomanesia phaea>/i< (O. Berg) Landrum)

Cultivo >i<in vitro>/i< de cerejeira-do-Rio-Grande (>i<Eugenia involucrata>/i< DC.) e de cambucizeiro (>i<Campomanesia phaea>/i< (O. Berg) Landrum)

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