Evaluation of rhizobacterial colonisation and the ability to induce Globodera pallida hatch

Lettice, Eoin P.; Jones, Peter

doi:10.1163/15685411-00002863

Cited by 5 publications

(5 citation statements)

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“…In the current context of the banishment of chemical nematicides, our results could be useful for potato breeding programs and for the establishment of new biocontrol products. First, wild tuber‐bearing Solanum of clade 4 could be preferred to develop products, based on root exudates, inducing the suicide hatch of juveniles in the absence of host plant (e.g., Devine & Jones, 2000; Lettice & Jones, 2015). Second, wild tuber‐bearing Solanum from clade 3 could be used by breeders for creating new potato cultivars, which would limit hatching of cysts and thus yield losses.…”

Section: Discussionmentioning

confidence: 99%

“…This result could be due to a differential adaptation of each G. pallida population to contrasted local climate conditions (i.e., temperature, humidity). Hence, the greatest hatching for this species occurred between 13 and 25°C Jones, 2015). Second, wild tuber-bearing Solanum from clade 3 could be used by breeders for creating new potato cultivars, which would limit hatching of cysts and thus yield losses.…”

Section: Discussionmentioning

confidence: 99%

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Plant–parasite coevolution: A weak signature of local adaptation between Peruvian Globodera pallida populations and wild potatoes

Gautier

Fournet

Piriou

et al. 2020

Ecology and Evolution

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Plant–parasite coevolution has generated much interest and studies to understand and manage diseases in agriculture. Such a reciprocal evolutionary process could lead to a pattern of local adaptation between plants and parasites. Based on the phylogeography of each partner, the present study tested the hypothesis of local adaptation between the potato cyst nematode Globodera pallida and wild potatoes in Peru. The measured fitness trait was the hatching of cysts which is induced by host root exudates. Using a cross‐hatching assay between 13 populations of G. pallida and root exudates from 12 wild potatoes, our results did not show a strong pattern of local adaptation of the parasite but the sympatric combinations induced better hatching of cysts than allopatric combinations, and there was a negative relationship between the hatching percentage and the geographical distance between nematode populations and wild potatoes. Moreover, a strong effect of the geographic origin of root exudates was found, with root exudates from south of Peru inducing better hatching than root exudates from north of Peru. These results could be useful to develop new biocontrol products or potato cultivars to limit damages caused by G. pallida.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Plant–parasite coevolution: A weak signature of local adaptation between Peruvian Globodera pallida populations and wild potatoes

Gautier

Fournet

Piriou

et al. 2020

Ecology and Evolution

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“… Ryan and Jones (2003) demonstrated that the spontaneous hatching of potato cyst nematodes (in the absence of plant or PRE) was higher in sand compared to in vitro , suggesting the occurrence of other HF produced by microorganisms. Lettice and Jones (2015) showed that isolated rhizobacteria, including Bacillus sp. , induced higher levels of G. pallida hatching in the absence of host plant than control.…”

Section: Discussionmentioning

confidence: 99%

“…Host detection mainly occurs through a broad range of signaling molecules released by roots. Some authors suggest to use this chemical signal, as biocontrol methods, to lure the parasites and thus to decrease their population in soil, as for example, for controlling parasitic weeds ( Zwanenburg et al, 2016 ), pathogens ( Rashid et al, 2013 ; Balendres et al, 2016 ), or nematodes ( Devine and Jones, 2000a ; Lettice and Jones, 2015 ). The principle of this strategy, namely suicide hatching, is to apply hatching stimulants into the soil to induce hatching of the parasite in absence of the host plant and thus its starvation and death.…”

Section: Introductionmentioning

confidence: 99%

Hatching of Globodera pallida Induced by Root Exudates Is Not Influenced by Soil Microbiota Composition

et al. 2020

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Plant-parasitic nematodes are among the most harmful pests of cultivated crops causing important economic losses. The ban of chemical nematicides requires the development of alternative agroecological approaches to protect crops against nematodes. For cyst nematodes, egg hatching is stimulated by host plant root exudates. Inducing “suicide hatching” of nematode second-stage juveniles (J2), using root exudates in the absence of the host plant, may constitute an effective and innovative biocontrol method to control cyst nematodes. However, before considering the development of this approach, understanding the effect of soil biotic component on cyst nematode hatching by root exudates is a major issue. The effectiveness of this approach could be modulated by other soil organisms consuming root exudates for growth as soil microbiota, and this must be evaluated. To do that, four different native agricultural soils were selected based on their physicochemical properties and their microbiota composition were characterized by rDNA metabarcoding. To disentangle the effect of microbiota from that of soil on hatching, four recolonized artificial soils were obtained by inoculating a common sterile soil matrix with the microbiota proceeding from each agricultural soil. Each soil was then inoculated with cysts of the potato cyst nematode, Globodera pallida , and low or high doses of potato root exudates (PREs) were applied. After 40 days, viable J2 remaining in cysts were counted to determine the efficiency of root exudates to stimulate hatching in different soils. Results showed that (i) when physicochemical and microbiota compositions varied among native soils, the hatching rates remained very high albeit small differences were measured and no dose effect was detected and (ii) when only microbiota composition varied among recolonized soils, the hatching rates were also high at the highest dose of PREs, but a strong dose effect was highlighted. This study shows that abiotic and biotic factors may not compromise the development of methods based on suicide hatching of cyst nematodes, using root exudates, molecules inducing J2 hatch, or trap crops.

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“…Among them, one may be very challenging and corresponds to the development of natural biocontrol products that induce the suicide hatch of the J2s in the absence of the host plant. The "suicide hatching" concept has already been explored and some authors proposed to use it to decrease population of parasites in the soil such as parasitic weeds (Zwanenburg et al, 2016), pathogens (Balendres et al, 2016;Rashid et al, 2013) or nematodes (Devine & Jones, 2000;Lettice & Jones, 2015). In cyst nematodes, the perception of chemical cues released by the host plant is an essential prerequisite to optimize nematode chances of successful infection through the synchronization of their life cycle with the presence of the host plant.…”

Section: Introductionmentioning

confidence: 99%

Impact of the genetic diversity of three cyst nematodes on the effectiveness of root exudates to induce hatching

et al. 2021

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Cyst nematodes are sedentary endoparasites of plants which cause important economic losses worldwide. New nematode control measures are needed since the removal of effective chemical nematicides from the market due to their negative impact on environment and human health.Induced hatching of second-stage juveniles in the absence of host plant using root exudates, also named "suicide hatching", could be a sustainable alternative method to reduce nematode population densities in infested soils. Unfortunately, biocontrol methods are often less effective in agricultural fields than in laboratory or greenhouse and this could be due to, among other reasons, a different susceptibility of nematode populations to root exudate stimulation. Testing this intra-specific variability would help to anticipate any drop in the efficiency of this new biocontrol strategy. A selection of root exudates from different plant species, maximizing the hatching level highlighted in a previous study, was tested on the hatching of representative populations of the genetic diversity for Globodera pallida, Heterodera schachtii and Heterodera carotae. Results showed significant differences between populations for given nematode species but not correlated with the genetic structure. Overall, root exudates tested provide a high level of hatching of these three nematode species. Surprisingly, the root exudate from broccoli induces hatching of European populations of the potato cyst nematode G. pallida.

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Evaluation of rhizobacterial colonisation and the ability to induce Globodera pallida hatch

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Cited by 5 publications

References 28 publications

Plant–parasite coevolution: A weak signature of local adaptation between Peruvian Globodera pallida populations and wild potatoes

Plant–parasite coevolution: A weak signature of local adaptation between Peruvian Globodera pallida populations and wild potatoes

Hatching of Globodera pallida Induced by Root Exudates Is Not Influenced by Soil Microbiota Composition

Impact of the genetic diversity of three cyst nematodes on the effectiveness of root exudates to induce hatching

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