Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State

Hayd, Ramão Luciano Nogueira; Carrara, Luana; Lima, Joel de Melo; Almeida, Nathalia Coelho Vargas de; Lima, José Bento Pereira; Martins, Ademir Jesús

doi:10.1186/s13071-020-04127-w

Cited by 13 publications

(20 citation statements)

References 24 publications

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“…Specimens with and without Wolbachia were used as positive and negative controls, respectively. kdr Genotyping Adult A. aegypti were genotyped by qPCR to detect single nucleotide polymorphisms (SNPs) at the 1,016 (Val + or Ile kdr ) and 1,534 (Phe + or Cys kdr ) positions of the voltage gated sodium channel gene (Na V ), as previously reported (Macoris et al, 2018;Hayd et al, 2020). Amplification reaction was performed with LightCycler 480 Probes Master mix (Roche), 10 ng of individual genomic DNA, and a set of primers and probes to detect kdr alleles (Supplementary Table S3) customized by Thermo Fisher Inc. under ID codes: AHS1DL6 (Val + 1016Ile kdr ) and AHUADFA (Phe + 1534 Cys ).…”

Section: Field Population Monitoring and Wolbachia Diagnosismentioning

confidence: 99%

Large-Scale Deployment and Establishment of Wolbachia Into the Aedes aegypti Population in Rio de Janeiro, Brazil

et al. 2021

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Traditional methods of vector control have proven insufficient to reduce the alarming incidence of dengue, Zika, and chikungunya in endemic countries. The bacterium symbiont Wolbachia has emerged as an efficient pathogen-blocking and self-dispersing agent that reduces the vectorial potential of Aedes aegypti populations and potentially impairs arboviral disease transmission. In this work, we report the results of a large-scale Wolbachia intervention in Ilha do Governador, Rio de Janeiro, Brazil. wMel-infected adults were released across residential areas between August 2017 and March 2020. Over 131 weeks, including release and post-release phases, we monitored the wMel prevalence in field specimens and analyzed introgression profiles of two assigned intervention areas, RJ1 and RJ2. Our results revealed that wMel successfully invaded both areas, reaching overall infection rates of 50–70% in RJ1 and 30–60% in RJ2 by the end of the monitoring period. At the neighborhood-level, wMel introgression was heterogeneous in both RJ1 and RJ2, with some profiles sustaining a consistent increase in infection rates and others failing to elicit the same. Correlation analysis revealed a weak overall association between RJ1 and RJ2 (r = 0.2849, p = 0.0236), and an association at a higher degree when comparing different deployment strategies, vehicle or backpack-assisted, within RJ1 (r = 0.4676, p < 0.0001) or RJ2 (r = 0.6263, p < 0.0001). The frequency knockdown resistance (kdr) alleles in wMel-infected specimens from both areas were consistently high over this study. Altogether, these findings corroborate that wMel can be successfully deployed at large-scale as part of vector control intervention strategies and provide the basis for imminent disease impact studies in Southeastern Brazil.

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Section: Field Population Monitoring and Wolbachia Diagnosismentioning

confidence: 99%

Large-Scale Deployment and Establishment of Wolbachia Into the Aedes aegypti Population in Rio de Janeiro, Brazil

et al. 2021

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“…Changes among genotypes within a couple of years were also observed in Ae. aegypti populations in Brazil 77 …”

Section: Discussionmentioning

confidence: 99%

“…aegypti populations in Brazil. 77 The V1016G and F1534C kdr mutations have been widely reported in Asia 47,78,79 and both have been implicated in reducing the sensitivity of VGSC to permethrin and deltamethrin. 27 An additional mutation, V410L, has also been reported in association with pyrethroid resistance in the Americas, but its presence in Asia has not been well studied 28 and we confirm that it is not yet present in Dhaka.…”

Section: Discussionmentioning

confidence: 99%

Insecticide resistance compromises the control of Aedes aegypti in Bangladesh

Al-Amin

Gyawali

Graham

et al. 2023

Pest Management Science

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BACKGROUND With no effective drugs or widely available vaccines, dengue control in Bangladesh is dependent on targeting the primary vector Aedes aegypti with insecticides and larval source management. Despite these interventions, the dengue burden is increasing in Bangladesh, and the country experienced its worst outbreak in 2019 with 101 354 hospitalized cases. This may be partially facilitated by the presence of intense insecticide resistance in vector populations. Here, we describe the intensity and mechanisms of resistance to insecticides commonly deployed against Ae. aegypti in Dhaka, Bangladesh. RESULTS Dhaka Ae. aegypti colonies exhibited high‐intensity resistance to pyrethroids. Using CDC bottle assays, we recorded 2–24% mortality (recorded at 24 h) to permethrin and 48–94% mortality to deltamethrin, at 10× the diagnostic dose. Bioassays conducted using insecticide–synergist combinations suggested that metabolic mechanisms were contributing to pyrethroid resistance, specifically multi‐function oxidases, esterases, and glutathione S‐transferases. In addition, kdr alleles were detected, with a high frequency (78–98%) of homozygotes for the V1016G mutation. A large proportion (≤ 74%) of free‐flying and resting mosquitoes from Dhaka colonies survived exposure to standard applications of pyrethroid aerosols in an experimental free‐flight room. Although that exposure affected the immediate host‐seeking behavior of Ae. aegypti, the effect was transient in surviving mosquitoes. CONCLUSION The intense resistance characterized in this study is likely compromising the operational effectiveness of pyrethroids against Ae. aegypti in Dhaka. Switching to alternative chemical classes may offer a medium‐term solution, but ultimately a more sustainable and effective approach to controlling dengue vectors is required. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…kdr genotyping Adult Ae. aegypti were genotyped by qPCR to detect single nucleotide polymorphisms (SNPs) at the 1016 (Val + or Ile kdr ) and 1534 (Phe + or Cys kdr ) positions of the voltage gated sodium channel gene (NaV), as previously reported (Macoris et al 2018;Hayd et al 2020). Amplification reaction was performed with LightCycler 480 Probes Master mix (Roche), 10ng of individual genomic DNA, and a set of primers and probes to detect kdr alleles (Supplementary Table S3) customized by Thermo Fisher Inc. under ID codes: AHS1DL6 (Val + 1016Ile kdr ) and AHUADFA (Phe + 1534 Cys ).…”

Section: Field Population Monitoring and Wolbachia Diagnosismentioning

confidence: 99%

Large-scale deployment and establishment ofWolbachiainto theAedes aegyptipopulation in Rio de Janeiro, Brazil

Gesto¹,

Pinto²,

Peixoto³

et al. 2021

Preprint

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Traditional methods of vector control have proven insufficient to reduce the alarming incidence of Dengue, Zika and chikungunya in endemic countries. The bacterium symbiont Wolbachia has emerged as an efficient pathogen-blocking and self-dispersing agent that reduces the vectorial potential of Aedes aegypti populations and potentially impairs arboviral disease transmission. In this work, we report the results of a large-scale Wolbachia intervention in Ilha do Governador, Rio de Janeiro, Brazil. wMel-infected adults were released across residential areas between August 2017 and March 2020. Over 131 weeks, including release and post-release phases, we monitored the wMel prevalence in field specimens, and analyzed introgression profiles of two assigned intervention areas, RJ1 and RJ2. Our results revealed that wMel successfully invaded both areas, reaching overall infection rates of 50-70% in RJ1, and 30-60% in RJ2 by the end of the monitoring period. At the neighborhood-level, wMel introgression was heterogeneous in both RJ1 and RJ2, with some profiles sustaining a consistent increase in infection rates and others failing to elicit the same. Correlation analysis revealed a weak overall association between RJ1 and RJ2 (r = 0.2849, P = 0.0236), and an association at a higher degree when comparing different deployment strategies, vehicle or backpack-assisted, within RJ1 (r = 0.4676, P < 0.0001) or RJ2 (r = 0.6263, P < 0.0001). The frequency knockdown resistance (kdr) alleles in wMel-infected specimens from both areas was consistently high over this study. Altogether, these findings corroborate that wMel can be successfully deployed at large-scale as part of vector control intervention strategies, and provide the basis for imminent disease impact studies in Southeastern Brazil.

show abstract

Evaluation of resistance to pyrethroid and organophosphate adulticides and kdr genotyping in Aedes aegypti populations from Roraima, the northernmost Brazilian State

Cited by 13 publications

References 24 publications

Large-Scale Deployment and Establishment of Wolbachia Into the Aedes aegypti Population in Rio de Janeiro, Brazil

Large-Scale Deployment and Establishment of Wolbachia Into the Aedes aegypti Population in Rio de Janeiro, Brazil

Insecticide resistance compromises the control of Aedes aegypti in Bangladesh

Large-scale deployment and establishment ofWolbachiainto theAedes aegyptipopulation in Rio de Janeiro, Brazil

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