Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry

Abstract

ObjectivesThe purpose of this study was to assess the ability of two new calcium silicate-based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA).Materials and MethodsEighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP).ResultsOn micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP.ConclusionsOur results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.

Bio-Inductive Materials in Direct and Indirect Pulp Capping—A Review Article

“…Development of minimally invasive biologically based therapies aimed at preservation of the pulp vitality remains the key theme within contemporary clinical endodontics. The present findings confirm that both MTA and Biodentine are reliable materials in the matter of inducing dentin bridge formation while keeping a vital pulp in both direct and indirect pulp capping procedures [71,104,108,112,140]. This review also reports Biodentine's superiority in relatively easier manipulation, lower cost and faster setting when compared to MTA with comparable or even outstanding clinical outcomes ( Table 2).…”

“…In terms of dentin bridge formation, based on the micro-CT imaging technique, the MTA group shows more a regular, homogenous reparative dentin layer with uniform thickness, when compared with the Biodentine group. These data indicate that both MTA and BD induced hard tissue barriers and that MTA induced dentin with better characteristics (Table 3) [71]. Therefore, MTA is the material of choice in direct pulp capping [70,72].…”

“…There is ample evidence for the positive effects of BD on vital pulp cells, for stimulating tertiary dentin formation and for early formation of reparative dentin [111]. Biodentine had a similar effect on dentin bridge formation to MTA (Table 3) [71,112,113]. In contrast to another study [46], a clinical trial in adults showed complete dentin bridge formation in 100% of Biodentine cases, compared to 11% and 56% in TheraCal®and ProRoot®MTA, respectively [114].…”

Influence of Biodentine® - A Dentine Substitute - On Collagen Type I Synthesis in Pulp Fibroblasts In Vitro

“…Furthermore it has been described that Biodentine ® as wells as MTA induced collagen fibre degradation [43]. In contrast to our observations increased collagen synthesis [44–46] and faster formation of new dentine [23, 47, 48] and induction of osteogenesis [49] have been described for calcium silicate cements. A reason for the versatile observations might be that the concentrations of Biodentine ® and/or the surface area exposed to culture media is not consistent in the described studies.…”

“…Biodentine ® is used as a dentine substitute in restorative dental therapy and as a capping agent in dentistry [21–23]. To characterize the effects caused by this tricalcium silicate based cement, primary pulp fibroblasts were treated for 24h with different Biodentine ® suspensions.…”