Evaluation of Rapid Neutralizing Antibody Detection Test against Rabies Virus in Human Sera

Nguyen, Kieu Anh Thi; Nguyen, Thu Tuyet; Nguyen, Dong Vinh; Ngo, Giang Chau; Nguyen, Cam Nhat; Yamada, Kentaro; Noguchi, Kazuko; Ahmed, Kamruddin; Hoang, Hanh Duc; Nishizono, Akira

doi:10.2149/tmh.2014-35

Cited by 7 publications

(5 citation statements)

References 9 publications

(14 reference statements)

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“…In order to detect rabies in the mongoose population in USVI, we assumed a prevalence of 10%-20% rabies exposed mongooses based on known prevalence data from the Caribbean [1,7,8]. The required sample size for presumed level of prevalence of disease in each population is shown in Table A in S1 Text, based on Bayesian methods that take into account the sensitivity (100%) and specificity (98.34%) [13] for the Rapid Fluorescent Foci Inhibition Test (RFFIT) on serum samples [14]. Direct fluorescent antibody (DFA) testing of brain stem and cerebellum samples is the gold standard for rabies virus antigen detection (100% sensitivity, 100% specificity) [15].…”

Section: Methodsmentioning

confidence: 99%

Determination of freedom-from-rabies for small Indian mongoose populations in the United States Virgin Islands, 2019–2020

et al. 2021

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Mongooses, a nonnative species, are a known reservoir of rabies virus in the Caribbean region. A cross-sectional study of mongooses at 41 field sites on the US Virgin Islands of St. Croix, St. John, and St. Thomas captured 312 mongooses (32% capture rate). We determined the absence of rabies virus by antigen testing and rabies virus exposure by antibody testing in mongoose populations on all three islands. USVI is the first Caribbean state to determine freedom-from-rabies for its mongoose populations with a scientifically-led robust cross-sectional study. Ongoing surveillance activities will determine if other domestic and wildlife populations in USVI are rabies-free.

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Section: Methodsmentioning

confidence: 99%

Determination of freedom-from-rabies for small Indian mongoose populations in the United States Virgin Islands, 2019–2020

et al. 2021

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“…Later, the mix is added to a target cell culture and incubated for 48 h (4). Finally, the GFP signal is measured by flow cytometry (5) Thi Nguyen et al (2015) modifications during the manufacturing process of the kit (Wasniewski et al 2014).…”

Section: Antigen Binding Assaysmentioning

confidence: 99%

“…In addition, RAPINA (rapid neutralizing antibody) test based on immunochromatography principle was proposed as a fast alternative method to detect the presence of neutralizing Abs in human serum samples (Shiota et al 2009). The latter could be an interesting alternative technique for initial screening methods for fieldwork (Thi Nguyen et al 2015).…”

Section: Antigen Binding Assaysmentioning

confidence: 99%

Detection and quantification of anti-rabies glycoprotein antibodies: current state and perspectives

Rodríguez

Fontana

Garay

et al. 2021

Appl Microbiol Biotechnol

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Rabies is an ancient fatal disease with no other available treatment than post-exposure vaccination, where the bite of infected animals, mainly dogs, is the leading cause of its transmission to human beings. In this context, global vaccination campaigns of companion animals, as well as wildlife reservoirs vaccination, are key factors to achieve the "Zero by 30" plan that pursues the eradication of dog-mediated human rabies by 2030. Rabies virus-neutralizing antibodies (VNAs) play an essential role in the disease protection, as it correlates with an adequate immune response and allows evaluating pre-or post-exposure prophylaxis efficacy. Hence, counting with reliable, accurate, and robust serological tests is of paramount importance. Currently, RFFIT and FAVN are the gold standard VNAs tests recommended by both the WHO and the OIE. Despite these methodologies are efficient and widely used, they present several drawbacks, as they are less easily to standardize and require the use of live rabies virus, containment facilities, and skilled professionals. Thus, in this review, we describe the state-of-the-art of alternative analytical methodologies currently available for rabies serology, with novel approaches based on pseudotyped recombinant viruses and emphasizing in the antigen binding methodologies that detect and quantify antibodies against the rabies glycoprotein. We discussed the wide range of assays that are interesting tools for a faster measurement of anti-rabies glycoprotein antibodies and, in some cases, less complex and more versatile than the gold standard methods. Finally, we discussed the key issues during the design and optimization steps of ELISA assays, highlighting the importance of validation and standardization procedures to improve rabies serology tests and, as a consequence, their results. Key points• An exhaustive revision of rabies serology testing was made.• No rabies serology assay can be thought as better than others for all intents and purposes.• The validation procedure guarantees reliable and consistent results among the globe.

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“…Inactivated cell culture vaccines are safe and easy to use and store. However, at least 3 injections are required to provide a sufficient virus neutralizing antibody (VNA) titer (at least 0.5 IU/ml), which is a reliable indicator that immune protection against rabies virus infection has been achieved [9,10]. In fact, the rabies neutralizing antibody titer is the most reliable indicator of immune protection [11].…”

Section: Introductionmentioning

confidence: 99%

“…Similar circumstances exist for humans. Most human rabies vaccinations are given post-exposure [9], so http://dx.doi.org/10.1016/j.ijbiomac.2016.02.023 0141-8130/© 2016 Elsevier B.V. All rights reserved. the rapid generation of an antibody response following rabies vaccination is very important.…”

Section: Introductionmentioning

confidence: 99%

Isatis indigotica root polysaccharides as adjuvants for an inactivated rabies virus vaccine

Zhang

Zheng

Cheng

et al. 2016

International Journal of Biological Macromolecules

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Adjuvants can enhance vaccine immunogenicity and induce long-term enhancement of immune responses. Thus, adjuvants are important for vaccine research. Polysaccharides isolated from select Chinese herbs have been demonstrated to possess various beneficial functions and excellent adjuvant abilities. In the present study, the polysaccharides IIP-A-1 and IIP-2 were isolated from Isatis indigotica root and compared with the common vaccine adjuvant aluminum hydroxide via intramuscular co-administration of inactivated rabies virus rCVS-11-G into mice. Blood was collected to determine virus neutralizing antibody (VNA) titers and B and T lymphocyte activation status. Inguinal lymph node samples were collected and used to measure B lymphocyte proliferation. Splenocytes were isolated, from which antigen-specific cellular immune responses were detected via ELISpot, ELISA and intracellular cytokine staining. The results revealed that both types of polysaccharides induce more rapid changes and higher VNA titers than aluminum hydroxide. Flow cytometry assays revealed that the polysaccharides activated more B lymphocytes in the lymph nodes and more B and T lymphocytes in the blood than aluminum hydroxide. Antigen-specific cellular immune responses showed that IIP-2 strongly induced T lymphocyte proliferation in the spleen and high levels of cytokine secretion from splenocytes, whereas aluminum hydroxide induced proliferation in only a small number of lymphocytes and the secretion of only small quantities of cytokines. Collectively, these data suggest that the polysaccharide IIP-2 exhibits excellent adjuvant activity and can enhance both cellular and humoral immunity.

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Evaluation of Rapid Neutralizing Antibody Detection Test against Rabies Virus in Human Sera

Cited by 7 publications

References 9 publications

Determination of freedom-from-rabies for small Indian mongoose populations in the United States Virgin Islands, 2019–2020

Determination of freedom-from-rabies for small Indian mongoose populations in the United States Virgin Islands, 2019–2020

Detection and quantification of anti-rabies glycoprotein antibodies: current state and perspectives

Isatis indigotica root polysaccharides as adjuvants for an inactivated rabies virus vaccine

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