Isatis indigotica root polysaccharides as adjuvants for an inactivated rabies virus vaccine

Zhang, Weijiao; Zheng, Xiaodong; Cheng, Nan; Gai, Weiwei; Xue, Xiaolin; Wang, Yuxia; Gao, Yuwei; Shan, Jing; Wang, Cuiling; Xia, Xianzhu

doi:10.1016/j.ijbiomac.2016.02.023

Cited by 19 publications

(14 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ratio of the adjuvant to the total volume was 50:50 for alum adjuvant (volume v/v). The mice in three different doses groups (eight mice per group) of L-EPS were subcutaneously immunized respectively with 0.2 mL PBS containing 100, 200 or 400 μg of L-EPS (Lee, Hwang, & Yun, 2009;Zhang et al, 2016;Zhang et al, 2017) and 20 μg of OVA; in alum adjuvant group with 0.2 mL of alum adjuvant containing 20 μg of OVA; in L-EPS group with 0.2 mL of PBS containing 200 μg of L-EPS; in OVA group with 0.2 mL of PBS containing 20 μg of OVA; PBS-treated mice as a control group. Blood samples were collected from the retinal vein at day 0, 14 and 28.…”

Section: Immunization Proceduresmentioning

confidence: 99%

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Xiu

Zhang

et al. 2018

Food and Agricultural Immunology

View full text Add to dashboard Cite

Exopolysaccharides act as mediators of cross-talk between probiotics and the host. Here, we found that EPS derived from probiotic Lactobacillus casei WXD030 strain (L-EPS) could modulate immune responses in vitro and in vivo. L-EPS could significantly enhance the proliferation and phagocytic activity as well as induce the production of NO, TNF-α, IL-1β and IL-6 in RAW264.7 cells. Furthermore, L-EPS could induce the maturation of BMDCs. In addition, L-EPS could largely increase the titres of OVA-specific antibodies and markedly enhanced T cell proliferation. Notably, L-EPS also increased expression of IL-4 and INF-γ expression in CD4 + T cells. Consistently, when used as an adjuvant in vivo with the foot-and-mouth disease vaccine, L-EPS largely enhanced the FMDVspecific antibody production. Collectively, these results suggested that L-EPS derived from probiotic L. casei strain had adjuvant activity, which may be a safe and efficacious adjuvant candidate suitable for a wide spectrum of prophylactic and therapeutic vaccines.

show abstract

Section: Immunization Proceduresmentioning

confidence: 99%

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Xiu

Zhang

et al. 2018

Food and Agricultural Immunology

View full text Add to dashboard Cite

show abstract

“…INR has a significant effect on chronic myelocytic leukemia (CML), and a possible mechanism has been investigated in animal studies (Mohan et al, 2018). Analogously, the pharmacological activities of nucleosides, organic acids, amino acids, and polysaccharide in I.indigotica have also been widely investigated (Chen et al, 2009;Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Untargeted liquid chromatography coupled with mass spectrometry reveals metabolic changes in nitrogen-deficient Isatis indigotica Fortune

Cao

Miao

et al. 2019

Phytochemistry

View full text Add to dashboard Cite

A B S T R A C TIsatis indigotica Fortune is a popular herb in traditional Chinese medicine, and various types of metabolites are the basis for its pharmacological efficacy. The biosynthesis and accumulation of these metabolites are closely linked to nitrogen availability; the benefits of low nitrogen application on the environment and herb quality are increasingly prominent. To analyze metabolic changes in the leaves and roots of I.indigotica in nitrogen deficiency conditions, and to identify the pathways and metabolites induced by low nitrogen availability, we used untargeted liquid chromatography coupled with mass spectrometry (UHPLC-TripleTOF) to obtain metabolomics profiling of I.indigotica under two N-deficiency treatments (0 kg/hm 2 ; 337.5 kg/hm 2 ) and normal nitrogen treatment (675 kg/hm 2 ). A total of 447 metabolites were annotated. Principal component analysis separated the three nitrogen treatments. A greater diversity of metabolites was observed in roots than in leaves under Ndeficiency treatments, suggesting that roots have a more important function in low N tolerance. Differential metabolites were mainly enriched in purine metabolism, phenylpropanoid biosynthesis, the shikimate pathway, tryptophan metabolism, and flavonoid biosynthesis that notably induced only in leaves in low nitrogen stress. Moderate N-deficiency benefits carbohydrate accumulation, whereas accumulation of most amino acids decreases. Uniquely, L-tryptophan was maintained at a high concentration in N-deficiency conditions. Low nitrogen stress induced the accumulation of some specialized metabolites (matairesinol, dictamnine, 5-hydroxyindoleacetate (serotonin) in roots and vitexin, xanthohumol, sinapyl alcohol in leaves). N-deficiency also increased the accumulation of adenosine and quality indicators of I.indigotica (indirubin-indigo, epigoitrin and anthranilic acid) in a certain degree. Our findings showed that nitrogen deficiency modified roots and leaves conditions of I.indigotica, affecting both the primary and secondary metabolism. Moderate nitrogen reduction was beneficial to the accumulation of active ingredients. Our methods and analysis are expected to provide an insight regarding the diversity of metabolites and regulation of their synthesis in low nitrogen application, and better investigate the nitrogen deficiency effect on I.indigotica.

show abstract

“…2,4,14,4, Crude total polysaccharides or two purified polysaccharides, PCP-I and PCP-II from Poria cocos could enhance the humoral responses to the H1N1 influenza and HBsAg vaccines. 14,24,26 To better characterize the immuneenhancing ability of PCP-I, in the present study we used the PA of B. anthracis as a model antigen, and analyzed systematically the adjuvant capabilities of PCP-I to enhance the immunogenicity and protection of an anthrax protective antigen-based vaccine.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharide PCP-I isolated from Poria cocos enhances the immunogenicity and protection of an anthrax protective antigen-based vaccine

Liu

Yin

Zhang

et al. 2019

Human Vaccines & Immunotherapeutics

Self Cite

View full text Add to dashboard Cite

Polysaccharides isolated from natural plants may represent a novel source of vaccine adjuvants. In this research, we focused on a natural plant polysaccharide, PCP-I, which is derived from Poria cocos, a Chinese traditional herbal medicine. We chose the anthrax protective antigen (PA) as a model to evaluate the adjuvant ability of PCP-I in enhancing the immunogenicity and protection of a PA-based anthrax vaccine. According to our results, PCP-I could significantly enhance anthrax specific anti-PA antibodies, toxin-neutralizing antibodies, anti-PA antibody affinity, as well as IgG1 and IgG2a levels. Besides, PCP-I increased the frequency of PA-specific memory B cells, increased the proliferation of PAspecific splenocytes, significantly stimulated the secretion of IL-4, and enhanced the activation of Dendritic cells (DCs) in vitro. The combination of PCP-I and CpG significantly enhanced the level of anti-PA antibodies and neutralizing antibodies, particularly PA-specific IgG2a, and shifted the Th2-bias to a Th1/Th2 balanced response. In addition, PCP-I with or without CpG could significantly improve the survival rate of immunized mice following challenge with the anthrax lethal toxin. These findings suggest that PCP-I may be a promising vaccine adjuvant that warrants further study.

show abstract

Isatis indigotica root polysaccharides as adjuvants for an inactivated rabies virus vaccine

Cited by 19 publications

References 40 publications

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Untargeted liquid chromatography coupled with mass spectrometry reveals metabolic changes in nitrogen-deficient Isatis indigotica Fortune

Polysaccharide PCP-I isolated from Poria cocos enhances the immunogenicity and protection of an anthrax protective antigen-based vaccine

Contact Info

Product

Resources

About