Evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting Gram-negative rods

Bombicino, Karina; Almuzara, Marisa; Famiglietti, Ángela; Vay, Carlos

doi:10.1016/j.diagmicrobio.2006.02.012

Cited by 12 publications

(9 citation statements)

References 20 publications

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“…Three different strains of bacteria including MRSA, carbapenem‐resistant A. baumannii and carbapenem‐resistant P. aeruginosa isolated from blood culture samples from bacteremia patients in the Gulhane Medical Faculty were used in this study. These isolates were identified by conventional methods [25, 26] and further by the API ID32GN system (bioMerieux, Marcy L’Etoile, France) and API Rapid ID 32 Staph system (bioMerieux). Staphylococcus aureus ATCC 29123, P. aeruginosa ATCC 27853 were used as control strains while vancomycin and imipenem were used as the control antimicrobial agents.…”

Section: Methodsmentioning

confidence: 99%

Melatonin as an antibiotic: new insights into the actions of this ubiquitous molecule

Tekbaş¹,

Ogur²,

Korkmaz³

et al. 2007

Journal of Pineal Research

129

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The incidence of serious infections caused by multidrug-resistant gram-positive and gram-negative bacteria has been increasing rapidly worldwide despite advances in antibacterial therapy in the last two decades. Among multidrug-resistant gram-positive and gram-negative bacteria, methicillin-resistant Staphylococcus aureus, carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii are of great importance, because they have emerged as primary nosocomial pathogens in hospital outbreaks. In this study, we investigated whether melatonin has antibacterial effects against these microorganisms in vitro. The minimum inhibitory concentration of melatonin was determined using a standard microdilution method at 24 and 48 hr. Melatonin inhibited microbial growth at both 24 and 48 hr; but results showed that melatonin had antibacterial effects against these microorganisms after 48 hr of incubation in lower doses [concentrations between 31.25 to 125 microg/mL (0.13-0.53 mM)]. Also, it was determined that melatonin has a more potent antimicrobial effect on gram-negative microorganism. Among possible mechanisms, it is concluded that melatonin showed antibacterial effects by reducing intracellular substrates.

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Section: Methodsmentioning

confidence: 99%

Melatonin as an antibiotic: new insights into the actions of this ubiquitous molecule

Tekbaş¹,

Ogur²,

Korkmaz³

et al. 2007

Journal of Pineal Research

129

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“…There are few biochemical differences between M. catarrhalis and M. nonliquefaciens, which are differentiated from each other mainly on the basis of morphological characteristics and by nitrite reductase and DNase activity of M. catarrhalis.M. atlantae is the only Moraxella species to be pyrrolidonyl aminopeptidase(17) positive. M. lacunata is the only proteolytic species with gelatinase activity.…”

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confidence: 97%

Acinetobacter, Chryseobacterium, Moraxella, and Other Nonfermentative Gram-Negative Rods

Vaneechoutte¹,

Dijkshoorn²,

Nemec³

et al. 2011

Manual of Clinical Microbiology

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A. beijerinckii (15) A. bereziniae (16) A. guillouiae (17) A. gyllenbergii (9) A. haemolyticus (21) A. junii (21) A. johnsonii (20) A. lwoffii (26) A. parvus (14) A. radioresistens (22) A. schindleri (22) A. ursingii (29) Growth at: 44°C D b 2 COLLECTION, TRANSPORT, AND STORAGE OF SPECIMENS Standard methods for collection, transport, and storage of specimens as detailed in chapters 9 and 16 are satisfactory for this group of organisms. The only fastidious species handled in this chapter are Asaia species, Granulibacter bethesdensis, Methylobacterium species, and some Moraxella species.

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“…El desoxicolato de sodio, sal derivada del ácido desoxicólico, (C 24 H 40 O 4 ) lisa la pared de la bacteria, por lo tanto, se considera una autolisina (enzima degradativa de peptidoglicano) que es liberada por la bilis a partir de la membrana de la célula y se une a los ácidos teicoicos que contienen colinas unidas al peptidoglicano (36). La autolisina entonces digiere la pared bacteriana celular resultando en lisis de la célula.…”

Section: Solubilidad En Bilisunclassified

Catalisis, enzimas y pruebas rápidas

Ramírez

Lozano

Duarte³

2022

nova

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Un gran número de los procesos metabólicos y biológicos son catalizados por enzimas; las enzimas son compuestos químicos orgánicos que pertenecen al grupo específico de las biomoléculas denominadas proteínas. Las enzimas poseen en su estructura molecular cuaternaria, organizaciones internas que permiten definir un lugar denominado centro activo; su función química, cinética y termodinámica se relacionan con la disminución de la energía de activación en el curso de la reacción neta. Los mecanismos de reacción enzimáticos que suceden en las interacciones metabólicas de los microorganismos han permitido desarrollar una serie de pruebas cualitativas que determinan la presencia o ausencia de bacterias en una muestra o un cultivo haciendo uso de técnicas rápidas que facilitan el diagnóstico clínico.

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Evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting Gram-negative rods

Cited by 12 publications

References 20 publications

Melatonin as an antibiotic: new insights into the actions of this ubiquitous molecule

Melatonin as an antibiotic: new insights into the actions of this ubiquitous molecule

Acinetobacter, Chryseobacterium, Moraxella, and Other Nonfermentative Gram-Negative Rods

Catalisis, enzimas y pruebas rápidas

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