Evaluation of Punica granatum Peels Extracts and its Phenolic, Alkaloid and Terpenoid Constituents Against Chemically Induced Diarrhoea in Rats

Abstract

| The present study was carried out to investigate the antidiarrhoeal activity of aqueous extract of peel of Punica granatum fruit and crude phenols, alkaloids, terpenoid extract in vivo by inducing diarrhoea in rats by oral administration of castor oil, enteropooling and small intestinal transit models in rats. The weight and volume of intestinal fluid induced by castor oil were studied by enteropooling method. Standard drug diphenoxylate (5 mg/kg, orally) was significantly reduced fecal output and frequency of droppings whereas crude phenols, alkaloids and aqueous extract, (at the doses of 20, 40 and 400 mg/kg orally respectively) significantly (P<0.001) reduced the castor oil induced frequency and consistency of diarrhoea and enteropooling. The gastrointestinal transit rate was expressed as the percentage of the longest distance travelled by the charcoal by the total length of the small intestine. Crude phenols, alkaloids and aqueous extract at the doses of 20, 40 and 400 mg/kg significantly inhibited (P<0.001) the castor oil induced charcoal meal transit. The crude phenols, alkaloids and aqueous extract of Punica granatum peel showed marked reduction in the number of diarrhoea stools and the reduction in the weight and volume of the intestinal contents, as well as a higher reduction in intestinal transit. Results obtained to establish the efficacy and substantiate the folklore claim as anti-diarrhoeal agent. Further studies are needed to understand the complete mechanism of antidiarrhoeal action of Punica ranatum.

Phytochemical Screening, Antioxidant Activity and Extraction of Active Compound (Anonaine) From Fruit Peel Extract of Annona Reticulata L.

“…This result is similar to Omulokoli et al [36] and Monica et al [37] where positive result obtained for phenols and alkaloids in high level and referred noted differences between plant parts in which alkaloids and flavonoids in peel and flower were higher than seeds and leaves. The findings were also supported by results [38] in which peel extract of Punica granatum was found maximum in phenol, alkaloid, and terpenoid content.…”

Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD

“…Between the extracts, the stem extract was more potent than leaves extract regarding the latent period and frequency of stool. Antidiarrhoeal activity of tannins, flavonoids, polyphenols, saponins, alkaloids, sterols, reducing sugars and triterpenes was reported previously [40][41][42]. In the phytochemical group tests alkaloids, glycosides, tannins, and flavonoids were identified in both extracts.…”

Antimicrobial Evaluation of Different Types of Aurantioideae Extracts Against Some Pathogenic Bacteria

“…Humans frequently consume citrus fruit juice because of its distinctive flavor and nutritional benefits. Juice's favorable health effects have been attributed in part to its abundance of vitamin C (ascorbic acid) (Ibrahim & Muhammad, 2016;Klimek-Szczykutowicz et al, 2020). Citrus fruits contain a wide range of bioactive substances, such as phenolics, flavonoids, vitamins, and essential oils.…”