Evaluation of Pulsed-Field Gel Electrophoresis Profiles for Identification of
            <i>Salmonella</i>
            Serotypes

Zou, Wen; Lin, Wei‐Jen; Foley, Steven L.; Chen, Chun-Houh; Nayak, Rajesh; Chen, James J.

doi:10.1128/jcm.00645-10

Cited by 48 publications

(41 citation statements)

References 23 publications

(18 reference statements)

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“…For banding-pattern-based subtyping methods, the ability to correctly predict serovars ranged from 65% to 76% for isolates representing the 40 most common Salmonella serovars, and by comparison, MLST correctly predicted the serovars of 91% of these isolates. Previous studies typically only tested the ability of one or a few subtyping methods to predict serovars in isolates representing limited diversity and a small number of serovars (19,21,22,(46)(47)(48). For example, Gaul et al (47) compared one banding-pattern method, PFGE, to traditional serotyping on a collection of 674 swine Salmonella isolates.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Typing Methods with a New Procedure Based on Sequence Characterization for Salmonella Serovar Prediction

et al. 2013

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bAs the development of molecular serotyping approaches is critical for Salmonella spp., which include >2,600 serovars, we performed an initial evaluation of the ability to identify Salmonella serovars using (i) different molecular subtyping methods and (ii) a newly implemented combined PCR-and sequencing-based approach that directly targets O-and H-antigen-encoding genes. Initial testing was performed using 46 isolates that represent the top 40 Salmonella serovars isolated from human and nonhuman sources, as reported by the U.S. Centers for Disease Control and Prevention and the World Health Organization. Multilocus sequence typing (MLST) was able to accurately predict the serovars for 42/46 isolates and showed the best ability to predict serovars among the subtyping methods tested. Pulsed-field gel electrophoresis (PFGE), ribotyping, and repetitive extragenic palindromic sequence-based PCR (rep-PCR) were able to accurately predict the serovars for 35/46, 34/46, and 30/46 isolates, respectively. Among the methods, S. enterica subsp. enterica serovars 4,5,12:i:؊, Typhimurium, and Typhimurium var. 5؊ were frequently not classified correctly, which is consistent with their close phylogenetic relationship. To develop a PCR-and sequence-based serotyping approach, we integrated available data sources to implement a combination PCR-based O-antigen screening and sequencing of internal fliC and fljB fragments. This approach correctly identified the serovars for 42/46 isolates in the initial set representing the most common Salmonella serovars, as well as for 54/63 isolates representing less common Salmonella serovars. Our study not only indicates that different molecular approaches show the potential to allow for rapid serovar classification of Salmonella isolates, but it also provides data that can help with the selection of molecular serotyping methods to be used by different laboratories.

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Section: Discussionmentioning

confidence: 99%

Comparison of Typing Methods with a New Procedure Based on Sequence Characterization for Salmonella Serovar Prediction

et al. 2013

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“…2); however, the identical banding pattern may not necessarily mean that the lymph node strains are related to liver abscess strains. Although PFGE is routinely used to assess strain relatedness in outbreak investigations of food-borne pathogens, in the case of Salmonella, at least with some serotypes, PFGE has proven to be serotype-specific and not strain-specific (Zou et al, 2010). It is not known whether S. enterica is one of the etiologic agents or a secondary invader into an abscess initiated by F. necrophorum, via lymph or blood, and survived.…”

Section: Bacteriologymentioning

confidence: 99%

Liver abscesses in cattle: A review of incidence in Holsteins and of bacteriology and vaccine approaches to control in feedlot cattle12

Amachawadi

Nagaraja

2016

Journal of Animal Science

111

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2 Adapted from Rezac et al. (2014b). Data collected at commercial packing plants in Kansas and Texas. 3 Adapted from Rezac et al. (2014a). Data collected at a commercial packing plant in the Great Lakes region.4 Normal = Liver free from abscesses; A-= Liver with 1 to 2 small abscesses; A = Liver with 2 to 4 large abscesses or multiple small abscesses; A+ = Liver with multiple large abscesses, abscesses adhered to diaphragm, abdominal organs or both, or open or ruptured abscesses.5 Numbers in parentheses are percentage of total abscesses.

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“…PFGE has been widely utilized in the molecular characterization of Salmonella species due to the stability of patterns, reproducibility and excellent discriminatory power (Ribot et al, 2002;Peters et al, 2003;Woo, 2005;Zou et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and resistance profile of Salmonella Enteritidis PT4 and PT9 strains isolated in Brazil

Kottwitz

Scheffer

Dalla-Costa

et al. 2011

Journal of Medical Microbiology

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A total of 41 Salmonella Enteritidis strains, including phago-types (PTs) PT4 and PT9, were characterized by antimicrobial resistance profiles and PFGE. Of these strains, 34 were isolated from patients and foods, and 7 were of poultry origin. All strains were susceptible to ampicillin, chloramphenicol, cefotaxime, ciprofloxacin and trimethoprim/sulfamethoxazole, and 41.5 % (n517) were resistant to nalidixic acid. PFGE analysis using XbaI and SpeI restriction enzymes resulted in X1S1 as the prevalent pattern, which was present in 48.8 % (n520) of epidemic strains and in one strain isolated from discarded hatching eggs. Distinct patterns were found for the other strains isolated from poultry (X3S1, X8S8, X11S12, X11S13, X16S1 and X13S15). The S. Enteritidis PT9 strains associated with outbreaks of salmonellosis were highly similar (¢0.90), suggesting clonality. The PFGE genotypes were related to the PTs, and it was possible to differentiate strains isolated from patients with salmonellosis from other strains of non-epidemic origin. The PFGE results suggested that the S. Enteritidis strains of poultry origin were a possible source of human salmonellosis during the study period. INTRODUCTIONSalmonella species are associated with food-borne diseases worldwide. Foods of animal origin, such as poultry meat, eggs and their products, are frequently implicated in outbreaks (Clavijo et al., 2006). In the period 1999-2008, Salmonella species were responsible for 1408 (23.2 %) of the 6062 outbreaks investigated in Brazil, and Salmonella species were responsible for 267 (43. According to epidemiological data from the Central Laboratory of Parana State (LACEN), egg products were associated with 45.0 % of the salmonellosis outbreaks occurring from 1999 to 2008, and Salmonella enterica subsp. enterica serovar Enteritidis was the predominant serovar found in patient samples (87.8 %) and foods (80.6 %) .Phenotypic methods, such as phago-typing, antimicrobial resistance profiling and molecular methods, including analysis of the macrorestriction pattern of chromosomal DNA after PFGE, have been used to characterize S. Enteritidis strains. PFGE has been widely utilized in the molecular characterization of Salmonella species due to the stability of patterns, reproducibility and excellent discriminatory power (Ribot et al., 2002;Peters et al., 2003;Woo, 2005;Zou et al., 2010).Salmonella strains isolated from different sources in the same temporal period and from the same geographical location are likely to be epidemiologically related and to have an identical PFGE restriction pattern independent of the restriction enzyme employed (Tenover et al., 1995). PFGE is capable of discriminating strains of Salmonella of the same serovar and phago-type (PT) (Ahmed et al., 2000; Liebana et al., 2001).The aim of the present study was to characterize S. Enteritidis PT4 and PT9 strains of poultry origin or isolated from patients and foods involved in salmonellosis outbreaks by antimicrobial resistance profiles and PFGE patterns. An epidemiological co...

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Evaluation of Pulsed-Field Gel Electrophoresis Profiles for Identification of Salmonella Serotypes

Cited by 48 publications

References 23 publications

Comparison of Typing Methods with a New Procedure Based on Sequence Characterization for Salmonella Serovar Prediction

Comparison of Typing Methods with a New Procedure Based on Sequence Characterization for Salmonella Serovar Prediction

Liver abscesses in cattle: A review of incidence in Holsteins and of bacteriology and vaccine approaches to control in feedlot cattle12

Molecular characterization and resistance profile of Salmonella Enteritidis PT4 and PT9 strains isolated in Brazil

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