Evaluation of Pseudomonas aeruginosa biofilm formation using piezoelectric tuning fork mass sensors

Waszczuk, K.; Guła, Grzegorz; Świątkowski, Michał; Olszewski, J.; Herwich, W.; Drulis-Kawa, Zuzanna; Gutowicz, Jan; Gotszalk, Teodor

doi:10.1016/j.snb.2010.11.019

Cited by 40 publications

(29 citation statements)

References 15 publications

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“…The equivalent circuit modelling of impedance spectra obtained using QTFs used as the impedance sensors allowed to determine two parameters which changed in presence of bacteria. Results were in agreement with previously published [4] obtained using classical standard growth curve method and by mass measurements using QTFs and colorimetric assay.…”

Section: Discussionsupporting

confidence: 92%

“…That change was correlated in time with the start of the cells adhesion to the surface of the QTF (Figs. 6 and 7) as well as the lowering of the resonance frequency of QTF caused by biofilm formation presented in the previous work [4]. The changes of the medium conductivity caused by the altering of electric properties of medium also resulted in the increase of capacitance of the CPE surf (Fig.…”

Section: Equivalent Circuit Analysissupporting

confidence: 56%

“…On the other hand, after the time in which in the mass measurements of the biofilm showed the gradual lowering of the detected mass [4] and the thickness of the biofilm decreased (Fig. 6) the significant rise of the Q parameter of CPE d was observed (Fig.…”

Section: Equivalent Circuit Analysismentioning

confidence: 93%

“…Previous work pointed that one of them may be to detect the formation of biofilm on its surface by measuring the shift of resonance frequency caused by the mass of adhered biofilm [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Pseudomonas aeruginosa biofilm formation using Quartz Tuning Forks as impedance sensors

Piasecki

Guła

Nitsch

et al. 2013

Sensors and Actuators B: Chemical

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Section: Discussionsupporting

confidence: 92%

Section: Equivalent Circuit Analysissupporting

confidence: 56%

Section: Equivalent Circuit Analysismentioning

confidence: 93%

“…Previous work pointed that one of them may be to detect the formation of biofilm on its surface by measuring the shift of resonance frequency caused by the mass of adhered biofilm [4,5].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Pseudomonas aeruginosa biofilm formation using Quartz Tuning Forks as impedance sensors

Piasecki

Guła

Nitsch

et al. 2013

Sensors and Actuators B: Chemical

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“…Due to the small dimension, low stiffness and high operating frequencies, excellent attributes and resolutions of mass change and force investigations can be achieved. For characterization of surface properties at the nanoscale, atomic force microscope (AFM) has been widely used [2][3][4][5]. Since the AFM and its applications are based on the interaction between an extremely sharp probe and the sample, the probe surface is one of the crucial concerns.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of boron-doped nanocrystalline diamond-coated MEMS probes

et al. 2016

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Fabrication processes of thin boron-doped nanocrystalline diamond (B-NCD) films on silicon-based micro-and nano-electromechanical structures have been investigated. B-NCD films were deposited using microwave plasma assisted chemical vapour deposition method. The variation in B-NCD morphology, structure and optical parameters was particularly investigated. The use of truncated cone-shaped substrate holder enabled to grow thin fully encapsulated nanocrystalline diamond film with a thickness of approx. 60 nm and RMS roughness of 17 nm. Raman spectra present the typical boron-doped nanocrystalline diamond line recorded at 1148 cm -1 . Moreover, the change in mechanical parameters of silicon cantilevers over-coated with boron-doped diamond films was investigated with laser vibrometer. The increase of resonance to frequency of over-coated cantilever is attributed to the change in spring constant caused by B-NCD coating. Topography and electrical parameters of boron-doped diamond films were investigated by tapping mode AFM and electrical mode of AFM-Kelvin probe force microscopy (KPFM). The crystallite-grain size was recorded at 153 and 238 nm for boron-doped film and undoped, respectively. Based on the contact potential difference data from the KPFM measurements, the work function of diamond layers was estimated. For the undoped diamond films, average CPD of 650 mV and for borondoped layer 155 mV were achieved. Based on CPD values, the values of work functions were calculated as 4.65 and 5.15 eV for doped and undoped diamond film, respectively. Boron doping increases the carrier density and the conductivity of the material and, consequently, the Fermi level.

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Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

Lapitan,

Felisilda,

Tiangco

et al. 2024

Chemistry An Asian Journal

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Pseudomonas aeruginosa is an opportunistic pathogen that infects wounds and burns and causes severe infections in humans. The high virulence, the emergence of antibiotic‐resistant strains, and the easy transmissibility of P. aeruginosa necessitate its fast detection and control. The gold standard for detecting P. aeruginosa, the plate culture method, though reliable, takes several days to complete. Therefore, developing accurate, rapid, and easy‐to‐use diagnostic tools for P. aeruginosa is highly desirable. Nanomaterial‐based biosensors are at the forefront of detecting P. aeruginosa and its secondary metabolites. This review summarises the biorecognition elements, biomarkers, immobilisation strategies, and current state‐of‐the‐art biosensors for P. aeruginosa. The review highlights the underlying principles of bioreceptor layer engineering and the design of nanomaterial‐based optical, electrochemical, mass‐based, and thermal biosensors. The advantages and disadvantages of these biosensors and their future point‐of‐care applications are also discussed.

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Evaluation of Pseudomonas aeruginosa biofilm formation using piezoelectric tuning fork mass sensors

Cited by 40 publications

References 15 publications

Evaluation of Pseudomonas aeruginosa biofilm formation using Quartz Tuning Forks as impedance sensors

Evaluation of Pseudomonas aeruginosa biofilm formation using Quartz Tuning Forks as impedance sensors

Fabrication and characterization of boron-doped nanocrystalline diamond-coated MEMS probes

Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

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