Evaluation of protein in vitro digestibility of Palmaria palmata and Gracilaria verrucosa

Abstract

Palmaria palmata and Gracilaria verrucosa are edible red seaweeds and potential protein sources for human or animal nutrition, so studies were conducted on their in vitro protein digestibility. After 30 min predigestion by pepsin followed by 6 h digestion into a cell dialysis containing porcine pancreatin, the in vitro protein digestibility of P. palmata and G. verrucosa, expressed in regard to casein digestibility, was 4.9% and 42.1%, respectively. The level of protein digestibility seems to be related to the amount of soluble fibre, which was 45.3% and 30.5%, respectively.

In vitro digestion of microalgal biomass from freshwater species isolated in Alberta, Canada for monogastric and ruminant animal feed applications

“…The in vivo digestibility of crude fiber in C. vulgaris biomass was found to be low at 37-41% in rats [19]. Not only does fiber reduce the overall algal biomass digestibility, high levels can also reduce protein digestibility by entrapping them in a cellular matrix which reduces their solubility, ultimately rendering them less available to proteolytic enzymatic hydrolysis [54]. This is consistent with our PS data, where N. bacillaris and Tetracystis sp., with high fiber levels, had consistently lower PS at an average of 65% than C. vulgaris and M. reisseri which averaged 77% with lower levels of fiber.…”

Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors

“…The lower IVPD observed for A. dimorphus is consistent with Subbulakshmi et al (1976) who also found lower IVPD for a related strain (Scenedesmus acutus=Scenedesmus obliquus) and may be related to its rigid cell wall (Miranda et al 2012). Reduced protein digestibility of algae can also be attributed to relatively high levels of soluble polysaccharide fiber that can entrap proteins in the cellular matrix, rendering them less bioavailable to enzymatic hydrolysis (Marrion et al 2005) and also to inhibitory effects of TPC (Mabeau and Fleurence 1993;Bobin-Dubigeon et al 1997). For the majority of microalgae studied here, IVPD was mid-range to high (>82 %) and TPC was generally low (<20 mg GAE g −1 DW; Table 1); however, no significant correlation between TPC and IVPD was found (r=−0.12, R 2 =0.0145, P=0.497).…”

Evaluation of Nutritive Value of Yam Based Weaning Food Fortified With Soy and Vegetables Minerals Sources

“…Mineral bioavailability was estimated by their digestibility in simulated physiological condition "in vitro" using a method proposed by Bermejo and others [12].…”

“…The mixtures were incubated for 2 hours at 37°C with gentle stirring constantly. The mixtures were then placed on ice until cool, then transferred in full in tubes and centrifuged at 10 000g for 30 minutes at 4°C Bermejo and others [12]. The supernatant recovered in silica capsules and minerals released during digestion were measured.…”