Evaluation of Production Parameters for Maximum Lipase Production by <i>P. stutzeri</i> MTCC 5618 and Scale-Up in Bioreactor

Thakur, Vishal; Tewari, Rupinder; Sharma, Rajat

doi:10.1155/2014/208462

Cited by 21 publications

(13 citation statements)

References 26 publications

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“…Others factors interfere in enzymatic production are medium conditions like temperature, pH and agitation (Willerding et al 2011, Thakur et al 2014. In this present study, once the bacteria have been isolated from industrial effluents, we opted to define typical conditions of environmental microorganisms, and so the incubation temperature for isolation and production of enzyme was 30ºC.…”

Section: Resultsmentioning

confidence: 99%

Bioprospecting of lipolytic microorganisms obtained from industrial effluents

Peil

Kuss

Rave

et al. 2016

An. Acad. Bras. Ciênc.

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The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identified using Gram stain and biochemical tests (Vitek 2®). Fungi were identified according to macro and micromorphology characteristics. The extracellular lipase production was evaluated using the Rhodamine B test and the enzymatic activity by titration. Twenty-one bacteria were isolated and identified as Klebsiella pneumoniae ssp. pneumoniae, Serratia marcescens, Enterobacter aerogenes, Raoultella ornithinolytica and Raoultella planticola. Were characterized isolated filamentous fungi by the following genera: Alternaria sp., Fusarium sp., Geotrichum sp., Gliocladium sp., Mucor sp., Paecilomyces sp. and Trichoderma sp. Extracellular lipase production was observed in 71.43% of the bacteria and 57.14% of the fungi. The bacterium that presented better promising enzymatic activity was E. aerogenes (1.54 U/ml) however between fungi there was not significant difference between the four isolates. This study indicated that microorganisms lipase producers are present in the industrial effluents, as well as these enzymes have potential of biodegradation of lipid compounds.

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Section: Resultsmentioning

confidence: 99%

Bioprospecting of lipolytic microorganisms obtained from industrial effluents

Peil

Kuss

Rave

et al. 2016

An. Acad. Bras. Ciênc.

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“…The use of high inoculum volumes (above 10%) in biomass production can cause some difficulties during the process. More precisely, the greater number of cells in production media the more competition for substrate occurs and vice versa [37]. Taking into account the gained results, the selected inoculum size in further analysis for both tested microorganisms is set at 5 vol.%.…”

Section: The Influence Of Inoculum Volume On Biomass Productionmentioning

confidence: 99%

Novel denitrifying bacteria Pseudomonas stutzeri strain D1 - from isolation to the biomass production

Vidaković

Šovljanski

Vučurović

et al. 2019

CI&CEQ

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Article Highlights• A novel aerobic denitrifier, P. stutzeri D1 was isolated • P. stutzeri D1 has great capability to fully reduce 3g/L of nitrate in aerobic conditions• The optimal conditions for biomass scale-up was determined• The scale-up of biomass production of P. stutzeri ATCC 17588 and D1 strain was performed Abstract An aerobic denitrifier was newly isolated and identified by VITEK ® 2 Compact System and MALDI-TOF MS as P. stutzeri strain D1. Sequence amplification indicates that the denitrification genes napA, nirS, norB and nosZ are present in a novel strain D1, as well as in reference strain ATCC 17588. Strain D1 had capability to fully remove 3 g/L of nitrate (as KNO 3 ) in 48 h, while the reference strain completed this task in 60 h. Single factor experiments indicate that the optimal conditions for biomass production were: temperature of 30 °C, pH value of 7 and inoculum volume of 5 vol.%. Scaling up of biomass production of both denitrifiers was successfully performed in 3 and 7 L laboratory bioreactors by reaching 9 log CFU/mL of the viable cells. The results demonstrate the feasibility of using investigated P. stutzeri strains in denitrification processes and the simplicity of the up-scaling of biomass production for the treatment of large areas contaminated with nitrate.

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“…However, in bioreactor agitation speed and type are among most vital factors influencing the cell growth and enzyme production since agitation ensures proper mixing of oxygen in fermentation medium that eventually becomes accessible for bacterial cells. Low agitation effects the oxygen transfer rate in medium as proper mixing of air with medium FIGURE 5 Course of cultivation time for chitosanase activity (A), extracellular protein content (B) and biomass (viable cell count) (C) studied in shake flasks using un-optimized medium ( ), in shake flasks using optimized medium (▴) and in stirred tank bioreactor using optimized medium (▪) does not happen, whereas at high agitation, mechanical shearing may happen which may ultimately influence the biomass formation and enzyme production [30]. Therefore, optimization of operational parameters is necessary to obtain the results equivalent to or superior than shake flasks.…”

Section: Discussionmentioning

confidence: 99%

Optimization of chitosanase production by Bacillus mojavensis EGE‐B‐5.2i

et al. 2018

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Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest. The present study was conducted to improve chitosanase activity of an indigenous strain identified as Bacillus mojavensis. Initially, carbon and nitrogen sources were optimized by one-variable-at-a-time approach. Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD). PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production. CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L , respectively to achieve maximum chitosanase activity (21.1 U ml ). Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds. Lastly, 18.6 U ml chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.

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Evaluation of Production Parameters for Maximum Lipase Production by P. stutzeri MTCC 5618 and Scale-Up in Bioreactor

Cited by 21 publications

References 26 publications

Bioprospecting of lipolytic microorganisms obtained from industrial effluents

Bioprospecting of lipolytic microorganisms obtained from industrial effluents

Novel denitrifying bacteria Pseudomonas stutzeri strain D1 - from isolation to the biomass production

Optimization of chitosanase production by Bacillus mojavensis EGE‐B‐5.2i

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