Evaluation of production of xanthan gum utilizing the corn cob liquor as a carbon source in different strains of Xanthomonas campestres

Jesus, Meirielly S.; Araújo, Petherson; Silva, Tamara; Reis, Elisiane; Ruzene, Denise Santos; Silva, Daniel; Padilha, Francine Ferreira

doi:10.1186/1753-6561-8-s4-p211

Cited by 3 publications

(3 citation statements)

References 2 publications

(2 reference statements)

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“…The importance of effectively utilizing carbon sources is underscored by the fact that the carbon source accounts for 30% of the running cost of EPS production (Onilude et al, 2012;Oner, 2013). In an attempt to reduce overhead costs due to the high cost of carbon sources, researchers are currently experimenting on EPS production using lignocellulosic wastes such as sugarcane straw (Abdeshahian et al, 2020), corn cob (Jesus et al,2014), rice hull (Wang et al, 2014, and rice straw (Jazini et al, 2017) for EPS production. The results obtained so far indicate the selective utilization of glucose over xylose in these biomasses, potentially leading to the wastage of the hemicellulose fraction of these biomasses.…”

Section: Exopolysaccharidesmentioning

confidence: 99%

Bio-Prospecting Xylose-Utilizing, Exopolysaccharide (EPS)-Producing Bacteria and EPS Quantification through Submerged Fermentation using Xylose as the Major Carbon Source

Antia, U.,

Stephen, N.,

Umoh, V.

et al. 2024

UJMR

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Study’s Novelty/ Excerpt This study identified bacterial isolates from diverse environments capable of utilizing xylose for exo-polysaccharide (EPS) production, an area with limited prior research. Potential EPS producers were isolated and identified as Staphylococcus, Enterobacter, Kocuria, Klebsiella, Enterococcus, Serratia, and Burkholderia species using a salt-based medium with xylose as the primary carbon source. The highest EPS production was observed in Enterobacter cloacae D1, E. cloacae D2, Klebsiella oxytoca D2, and K. oxytoca G1, ranging from 0.04 g/L to 2.0 g/L, highlighting these isolates' potential for optimization and application in various industries. Full Abstract Many microorganisms are capable of producing Exo-polysaccharides (EPS) while utilizing simple sugars and hexoses. These EPS found applications in various fields, such as agricultural biotechnology, pharmaceuticals, textiles, and food industries. However, there is a lack of studies on EPS-elaborating bacteria that can utilize pentoses like xylose. Therefore, the utilization of alternative carbon sources for EPS production has become a focus of recent research. This study aimed to prospect bacteria that can utilize xylose for EPS production. Samples from agricultural soil, dump sites, saline soil, cement-contaminated soil, fresh cow milk, cow dung, and yogurt were serially diluted and cultured in a salt-based medium with xylose as the primary carbon source. Slimy and mucoid colonies were selected as potential EPS-producing isolates and identified morphologically and biochemically using the VITEK 2 Automated identification system. The quantification of EPS production by these isolates was conducted through submerged fermentation with xylose as the sole carbon source. The mean heterotrophic bacterial count of xylose-utilizing bacteria ranged from 2.1x106 CFU to 3.5x108 CFU per gram of the samples analyzed. The slimy and mucoid colonies were identified as members of the genera Staphylococcus, Enterobacter, Kocuria, Klebsiella, Enterococcus, Serratia, and Burkholderia. The quantities of EPS produced by the isolates ranged from 0.04 g/L to 2.0 g/L, with E. cloacae D1, E. cloacae D2, K. oxytoca D2, and K. oxytoca G1 elaborating the highest amount of EPS. Bacterial isolates capable of utilizing xylose for EPS production were obtained from various sources, showing potential for further optimization

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Section: Exopolysaccharidesmentioning

confidence: 99%

Bio-Prospecting Xylose-Utilizing, Exopolysaccharide (EPS)-Producing Bacteria and EPS Quantification through Submerged Fermentation using Xylose as the Major Carbon Source

Antia, U.,

Stephen, N.,

Umoh, V.

et al. 2024

UJMR

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“…The utilisation of lignocellulose as a carbon source will effectively maximise the cost‐effectiveness of polysaccharide production. However, glucose‐rich enzymatic hydrolysate and xylose‐rich acid/alkali hydrolysate were always used separately to produce polysaccharides in most reports (Abdeshahian et al., 2020; Jazini et al., 2017; Jesus et al., 2014; Wang et al., 2014). Sphingomonas sanxanigenens NX02 could naturally co‐utilise glucose and xylose from corn straw total hydrolysate to produce the polysaccharide Sanxan (Wu et al., 2021), which was a promising alternative in the food, cosmetic and petroleum industries because of its excellent gel‐forming, emulsifying and thickening properties (Wu et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

“…When cultured in a medium containing glucose and xylose, the strain always utilised glucose as a preferred carbon source (Zhang & Chen, 2010). Recently, xylose‐rich acid hydrolysate of broomcorn stem (Zahra et al., 2018), alkaline hydrolysate of corn cob liquor (Jesus et al., 2014) and glucose‐rich enzymatic hydrolysate of rice straw (Jazini et al., 2017) have been utilised as carbon sources to produce xanthan gum. Additionally, other different lignocellulose partial hydrolysates have also been used as carbon sources to produce xanthan gum, such as alkaline hydrolysed corncob (Jesus et al., 2023), acid hydrolysed Melaleuca alternifolia residue (Li et al., 2022), self‐hydrolysed coconut shells and cocoa husks under sterilisation conditions (da Silva et al., 2018), acid hydrolysed orange peels (Mohsin et al., 2018), and enzymatically hydrolysed potato crop residues (Soltaninejad et al., 2022), but the full utilisation of total hydrolysate rich in both glucose and xylose to produce xanthan gum has not been reported.…”

Section: Introductionmentioning

confidence: 99%

The production of ultrahigh molecular weight xanthan gum from a Sphingomonas chassis capable of co‐utilising glucose and xylose from corn straw

Wu,

Shi,

Ming

et al. 2024

Microbial Biotechnology

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Corn straw is an abundant and renewable alternative for microbial biopolymer production. In this paper, an engineered Sphingomonas sanxanigenens NXG‐P916 capable of co‐utilising glucose and xylose from corn straw total hydrolysate to produce xanthan gum was constructed. This strain was obtained by introducing the xanthan gum synthetic operon gum as a module into the genome of the constructed chassis strain NXdPE that could mass produce activated precursors of polysaccharide, and in which the transcriptional levels of gum genes were optimised by screening for a more appropriate promoter, P916. As a result, strain NXG‐P916 produced 9.48 ± 0.34 g of xanthan gum per kg of fermentation broth (g/kg) when glucose was used as a carbon source, which was 2.1 times improved over the original engineering strain NXdPE::gum. Furthermore, in batch fermentation, 12.72 ± 0.75 g/kg xanthan gum was produced from the corn straw total hydrolysate containing both glucose and xylose, and the producing xanthan gum showed an ultrahigh molecular weight (UHMW) of 6.04 × 107 Da, which was increased by 15.8 times. Therefore, the great potential of producing UHMW xanthan gum by Sphingomonas sanxanigenens was proved, and the chassis NXdPE has the prospect of becoming an attractive platform organism producing polysaccharides derived from biomass hydrolysates.

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Efficient simultaneous utilization of glucose and xylose from corn straw by Sphingomonas sanxanigenens NX02 to produce microbial exopolysaccharide

Zhao

Shen

et al. 2021

Bioresource Technology

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Evaluation of production of xanthan gum utilizing the corn cob liquor as a carbon source in different strains of Xanthomonas campestres

Cited by 3 publications

References 2 publications

Bio-Prospecting Xylose-Utilizing, Exopolysaccharide (EPS)-Producing Bacteria and EPS Quantification through Submerged Fermentation using Xylose as the Major Carbon Source

Bio-Prospecting Xylose-Utilizing, Exopolysaccharide (EPS)-Producing Bacteria and EPS Quantification through Submerged Fermentation using Xylose as the Major Carbon Source

The production of ultrahigh molecular weight xanthan gum from a Sphingomonas chassis capable of co‐utilising glucose and xylose from corn straw

Efficient simultaneous utilization of glucose and xylose from corn straw by Sphingomonas sanxanigenens NX02 to produce microbial exopolysaccharide

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