Efficient simultaneous utilization of glucose and xylose from corn straw by Sphingomonas sanxanigenens NX02 to produce microbial exopolysaccharide

Wu, Mengmeng; Zhao, Xin; Shen, Yaqi; Shi, Zhuangzhuang; Li, Guoqiang; Ma, Ting

doi:10.1016/j.biortech.2020.124126

Cited by 24 publications

(25 citation statements)

References 43 publications

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“…Amongst the various renewable resources that are currently being investigated, lignocellulosic biomass represents the largest net resource with a worldwide annual production of 200 billion tons (Simmons et al, 2008;Tuck et al, 2012). Industrial biotechnology, wherein microbial strains are utilized for coupled bioconversion of various carbon substrates present in biomass, is expected to provide a scalable and cost-competitive production method for renewable fuels and chemicals (Sievert et al, 2017;Flores et al, 2020;Abdel-Rahman et al, 2021;Flores et al, 2021;Wu et al, 2021;Zan et al, 2021). However, several challenges still need to be overcome for efficient bioconversion of biomass to bioproducts.…”

Section: Introductionmentioning

confidence: 99%

Corynebacterium glutamicum as an Efficient Omnivorous Microbial Host for the Bioconversion of Lignocellulosic Biomass

Mhatre

Shinde

Jha

et al. 2022

Front. Bioeng. Biotechnol.

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Corynebacterium glutamicum has been successfully employed for the industrial production of amino acids and other bioproducts, partially due to its native ability to utilize a wide range of carbon substrates. We demonstrated C. glutamicum as an efficient microbial host for utilizing diverse carbon substrates present in biomass hydrolysates, such as glucose, arabinose, and xylose, in addition to its natural ability to assimilate lignin-derived aromatics. As a case study to demonstrate its bioproduction capabilities, L-lactate was chosen as the primary fermentation end product along with acetate and succinate. C. glutamicum was found to grow well in different aromatics (benzoic acid, cinnamic acid, vanillic acid, and p-coumaric acid) up to a concentration of 40 mM. Besides, 13C-fingerprinting confirmed that carbon from aromatics enter the primary metabolism via TCA cycle confirming the presence of β-ketoadipate pathway in C. glutamicum. 13C-fingerprinting in the presence of both glucose and aromatics also revealed coumarate to be the most preferred aromatic by C. glutamicum contributing 74 and 59% of its carbon for the synthesis of glutamate and aspartate respectively. 13C-fingerprinting also confirmed the activity of ortho-cleavage pathway, anaplerotic pathway, and cataplerotic pathways. Finally, the engineered C. glutamicum strain grew well in biomass hydrolysate containing pentose and hexose sugars and produced L-lactate at a concentration of 47.9 g/L and a yield of 0.639 g/g from sugars with simultaneous utilization of aromatics. Succinate and acetate co-products were produced at concentrations of 8.9 g/L and 3.2 g/L, respectively. Our findings open the door to valorize all the major carbon components of biomass hydrolysate by using C. glutamicum as a microbial host for biomanufacturing.

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Section: Introductionmentioning

confidence: 99%

Corynebacterium glutamicum as an Efficient Omnivorous Microbial Host for the Bioconversion of Lignocellulosic Biomass

Mhatre

Shinde

Jha

et al. 2022

Front. Bioeng. Biotechnol.

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“…Despite the lower energy efficiency, the Weimberg pathway still presents a promising route to produce valuable TCA-cycle derivatives from d -xylose due to its high carbon efficiency [ 13 , 18 , 42 , 43 ]. Besides, it provides a parallel metabolic pathway to co-utilize glucose and xylose from hydrolysate of lignocellulosic biomass [ 6 , 7 ]. Furthermore, M. thermophila was also engineered to produce BT, showing the opportunity to synthesize chemicals directly from lignocellulosic biomass through XOP.…”

Section: Discussionmentioning

confidence: 99%

The Weimberg pathway: an alternative for Myceliophthora thermophila to utilize d-xylose

Liu

Zhang

et al. 2023

Biotechnol Biofuels

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Background With d-xylose being the second most abundant sugar in nature, its conversion into products could significantly improve biomass-based process economy. There are two well-studied phosphorylative pathways for d-xylose metabolism. One is isomerase pathway mainly found in bacteria, and the other one is oxo-reductive pathway that always exists in fungi. Except for these two pathways, there are also non-phosphorylative pathways named xylose oxidative pathways and they have several advantages over traditional phosphorylative pathways. In Myceliophthora thermophila, d-xylose can be metabolized through oxo-reductive pathway after plant biomass degradation. The survey of non-phosphorylative pathways in this filamentous fungus will offer a potential way for carbon-efficient production of fuels and chemicals using d-xylose. Results In this study, an alternative for utilization of d-xylose, the non-phosphorylative Weimberg pathway was established in M. thermophila. Growth on d-xylose of strains whose d-xylose reductase gene was disrupted, was restored after overexpression of the entire Weimberg pathway. During the construction, a native d-xylose dehydrogenase with highest activity in M. thermophila was discovered. Here, M. thermophila was also engineered to produce 1,2,4‐butanetriol using d-xylose through non-phosphorylative pathway. Afterwards, transcriptome analysis revealed that the d-xylose dehydrogenase gene was obviously upregulated after deletion of d-xylose reductase gene when cultured in a d-xylose medium. Besides, genes involved in growth were enriched in strains containing the Weimberg pathway. Conclusions The Weimberg pathway was established in M. thermophila to support its growth with d-xylose being the sole carbon source. Besides, M. thermophila was engineered to produce 1,2,4‐butanetriol using d-xylose through non-phosphorylative pathway. To our knowledge, this is the first report of non-phosphorylative pathway recombinant in filamentous fungi, which shows great potential to convert d-xylose to valuable chemicals.

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“…Current biological methods for treating xylose-rich wastewater include the use of microalgae, bacteria, and fungi. 15,16 Nevertheless, all these proposed biological approaches are easily contaminated by bacteria and will produce a peculiar smell. What's more, these processes are highly dependent on the purity of the waste stream and face several challenges before being scaled up.…”

Section: Introductionmentioning

confidence: 99%

Use of hemicellulose-derived xylose for environmentally sustainable starch production by mixotrophic duckweed

Sun

Zhao

Yang

et al. 2023

Sustainable Energy Fuels

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Efficient simultaneous utilization of glucose and xylose from corn straw by Sphingomonas sanxanigenens NX02 to produce microbial exopolysaccharide

Cited by 24 publications

References 43 publications

Corynebacterium glutamicum as an Efficient Omnivorous Microbial Host for the Bioconversion of Lignocellulosic Biomass

Corynebacterium glutamicum as an Efficient Omnivorous Microbial Host for the Bioconversion of Lignocellulosic Biomass

The Weimberg pathway: an alternative for Myceliophthora thermophila to utilize d-xylose

Use of hemicellulose-derived xylose for environmentally sustainable starch production by mixotrophic duckweed

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