Evaluation of process parameters in the production of jasmonic acid

Jasmonic acid biosynthesis by fungi: derivatives, first evidence on biochemical pathways and culture conditions for production

“…An early study showed that the addition of an inductor is not required to produce JA in synthetic culture medium ( Miersch et al, 1987 ). In fact, the addition of 18:3(n-3) (1 µM) ( Dos Santos et al, 2014 ) or edible oil (1 g L −1 ) ( Eng, 1996 ) as substrate and fatty acid source for JA synthesis to the culture media of Botryosphaeria rhodina strain Kinf 3.1 or Lasiodiplodia sp. strain 2,334, respectively, was not significantly favoured in the JA production.…”

“…However, scaling up JA production in a fermenter or in a shaking incubator at 190 rpm and 30 °C with a dissolved oxygen saturation in the culture medium of up to 150 L ( Farbood et al, 2001 ) as well as using a fixed inoculation ratio of 0.5 g L −1 of dry biomass of culture medium was shown to further improve JA yield ( Miersch et al, 1987 ). In addition, it was of advantage to use homogenized mycelium and not spores ( Almeida, Altuna & Michelena, 2001 ; Dos Santos et al, 2014 ; Dos Santos, Innocentini & Lourenco, 2014 ). Agitation turned out to be another critical aspect for JA production, because shaking speeds above 200 rpm lead to increased synthesis of extracellular polysaccharides that visibly increased the viscosity of the culture medium ( Selbmann, Crognale & Petruccioli, 2004 ), which had a negative effect on JA production ( Eng, Gutiérrez-Rojas & Favela-Torres, 1998 ; Miersch et al, 1987 ).…”

High production of jasmonic acid by Lasiodiplodia iranensis using solid‐state fermentation: Optimization and understanding

“…[ 13 ] In addition, Botryosphaeria rhodina fungus cultured on a tryptone‐free medium for 10 days yielded 421.5 mg L –1 of JA. [ 14 ] When some culture conditions were optimized, the strain L. theobromae MTCC No. 3068 produced 171–294 mg L –1 JA with productivity of about 43 mg L –1 per day.…”