Evaluation of pro-oxidant-antioxidant balance (PAB) and its association with inflammatory cytokines in polycystic ovary syndrome (PCOS)

Abstract

Chronic low-grade inflammation has been suggested as a key contributor of the pathogenesis and development of polycystic ovary syndrome (PCOS). To investigate the association between oxidative stress status and inflammatory cytokines in follicular fluid of 21 PCOS women compared to 21 women with normal ovarian function who underwent intra-cytoplasmic sperm injection. Concentration of IL-6, IL-8, IL-10, and TNF-α was measured using sandwich ELISA. Oxidative stress was examined by measuring total oxidant status (TOS), malondialdehyde (MDA), total antioxidant capacity (TAC), and thiol groups. PCOS women had an elevated concentration of MDA and TOS compared to controls. Levels of TAC and thiol groups were lower in PCOS compared to controls. PCOS patients had a higher concentration of IL-6, IL-8, and TNF-α compared to controls. Concentration of IL-10 was lower in PCOS compared to controls. Significant correlations were found between MDA and TOS concentration with TNF-α and between IL-6 and MDA, IL-8 and TAC, IL-10 and TOS levels and also between IL-10 and TAC levels. TAC and thiol groups were negatively correlated with TNF-α. Increased oxidative stress in PCOS is associated with inflammation which is closely linked. Inflammation can induce production of inflammatory cytokines in this syndrome and directly stimulates excess ovarian androgen production.

Inulin and metformin ameliorate polycystic ovary syndrome <i>via</i> anti-inflammation and modulating gut microbiota in mice

“…As critical pro-inflammatory indicators, abnormal high levels of plasma TNF-α and IL-6 have been demonstrated to promote the proliferation, differentiation and maturation of follicles in PCOS patients [25][26][27]. Alteration of TNF-α in FF levels is associated with poorquality oocytes undergoing IVF which decreased rates of fertilization, embryonic development, and pregnancy outcome [25].…”

“…IL-6 can reduce intra-follicular estradiol concentration, fertility and fertilizing capacity [26]. Studies have shown that pro-inflammatory cytokine (such as TNF-α and IL-6) in PCOS are significantly increased [27]. TNF-α, IL-6 and IL-10 play important role in the development of inflammation and oxidative stress [27].…”

“…Studies have shown that pro-inflammatory cytokine (such as TNF-α and IL-6) in PCOS are significantly increased [27]. TNF-α, IL-6 and IL-10 play important role in the development of inflammation and oxidative stress [27]. Serum IL-17A, TNF-α and IL-6 are closely related to the number of meiotic I (MI) oocytes in PCOS patients [28].…”

Polycystic Ovary Syndrome as a systemic disease with multiple molecular pathways: a narrative review

“…Oxidative stress. Artimani et al (2018) have evaluated follicular fluid samples of 21 PCOS women compared to 21 women with normal ovarian function. The oxidative stress was examined by measuring total oxidant status (TOS), malondialdehyde (MDA), total antioxidant capacity (TAC), and thiol groups.…”

Efeitos do treinamento físico aeróbio sobre a função sexual em mulheres com síndrome dos ovários policísticos: ensaio clínico controlado

“…Diante das alterações, as mulheres com SOP desenvolvem uma série de complicações, o que culmina com maior prevalência do aumento do peso e gordura corporal, elevação dos níveis de marcadores inflamatórios, aumento da resistência à insulina e diabetes tipo II, além de maiores chances de desenvolver a síndrome metabólica. (GROUP et al, 2004;GONZÁLEZ et al, 2012;LIM et al, 2013;THOMSON et al, 2012;BATISTA et al, 2014;YUAN et al, 2016;ARTIMANI, T. et al, 2017;BUSIAH et al, 2017;2017;DABBAGHMANESH et al, 2017). Além disso, tem sido demonstrado que mulheres com SOP apresentam maiores chances de alteração do estado emocional, de disfunção sexual, com redução da qualidade de vida e. (ELSENBRUCH et al, 2003;CINAR et al, 2011;FERRARESI et al, 2013;MOREIRA et al, 2013;EFTEKHAR et al, 2014;SHAFTI e SHAHBAZI, 2016;CESTA et al, 2017;KARJULA et al, 2017).…”