Evaluation of Preparation Methods for Scanning Electron Microscopic Observation of Plant Protoplasts.

Optical Microscopy and Scanning Electron Microscopy on the Surface of Rice Callus after Treatment with Cell Wall Degrading Enzymes

“…Dehydrated specimens were covered with gold under vacuum conditions and then examined with a Hitachi scanning electron microscope (S-415) operating at 25 kV. Details of these methods have been described elsewhere (Sato et al, 1995a).…”

“…Therefore, rice protoplasts have been successfully isolated from the chopped sections of roots and leaves and not from the epidermal layer (Maeda, 1971;Maeda and Hagiwara, 1974). Sato et al (1995a) isolated numerous protoplasts from the chopped samples of petunia leaves and rice calli, and further reported a practical fixation method of isolated protoplasts for SEM.…”