Microtopography and Shoot-bud Formation of Rice (Oryza sativa) Callus

Maeda, Eizo; Sato, Toyoyuki; Suzuki, Katsumi

doi:10.5511/plantbiotechnology.19.69

Cited by 4 publications

(5 citation statements)

References 61 publications

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“…A coleoptile is a protective sheath that surrounds the embryonic leaves. Cells of this organ possess larger vacuoles than the leaves (Taylor and Vasil , Maeda et al , Jones and Rost ). During 186 h in NaCl exposure, high concentrations of Na + and Cl – in coleoptiles (>200 µmol g –1 fresh weight at 200 m M NaCl in the medium) indicated that most of these ions could be stored in vacuoles which occupy the bulk of the intracellular volume.…”

Section: Discussionmentioning

confidence: 99%

Tolerance of submerged germinating rice to 50–200 mM NaCl in aerated solution

Kurniasih

Greenway

Colmer

2013

Physiologia Plantarum

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This paper concerns tolerance to 50-200 mM NaCl of submerged rice (Oryza sativa cv. Amaroo) during germination and the first 138-186 h of development in aerated solution. Rice was able to germinate and the seedlings even tolerated exposure to 200 mM NaCl, albeit with severe growth restrictions. After return to 0.3 mM NaCl, growth increased, indicating that even at 200 mM NaCl there was no irreparable injury. Osmotic adjustment was achieved by using Na⁺ and Cl⁻ as the major osmotica. At 200 mM NaCl commenced at sowing, the shoot Na⁺ and Cl⁻ concentrations between 50-110 h were about 210 and 260 mM, respectively, i.e. above the external concentration. Thus, there was a high tissue tolerance to NaCl. The internal concentrations declined subsequently, concurrent with a decline in growth. At 50-200 mM NaCl, the contributions from ions to πsap were 81-92% in roots and 62-74% in shoots. The assessed turgor pressures at 200 mM NaCl were 0.33 MPa in shoots and 0.15 MPa in roots, compared to 0.62 and 0.43 MPa at 0.3 mM NaCl. In the General Discussion section, we compare the different responses of submerged seedlings to the responses of transpiring rice plants, reported in the literature, and suggest that the submerged system is useful to evaluate effects of NaCl on turgor pressure and particularly to establish whether there are specific effects of Na⁺ and Cl⁻ in tissues.

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Section: Discussionmentioning

confidence: 99%

Tolerance of submerged germinating rice to 50–200 mM NaCl in aerated solution

Kurniasih

Greenway

Colmer

2013

Physiologia Plantarum

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“…Abe and Futsuhara (1991) and Ogawa et al (1999) argued that callus browning is genetically controlled, which correspond to the present study. A decrease in the rate of callus growth is occasionally related to the appearance of a brown colored area (Maeda et al, 2002). A low 2,4-D concentration (Mitsuoka et al, 1994) and high temperature (Hoque and Mansfi eld, 2004) seem to cause callus browning or regeneration of albino plants.…”

Section: Discussionmentioning

confidence: 99%

“…1) a soft, transparent, unorganized callus, which is nonmorphogenic or may form only roots, and 2) soft mucilaginous callus, which often form roots. Maeda (2000) and Maeda et al (2002) reported that white and green patches are often seen on the surface stratum of callus with high regeneration ability. Moreover, in sorghum, Kaeppler and Pedersen (1997) reported that the production of a high quality callus depends mainly on the genotype.…”

Section: Discussionmentioning

confidence: 99%

Identification of Callus Induction Potential of 15 Indonesian Rice Genotypes

Carsono

Yoshida

2006

Plant Production Science

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“…In this study, the effect of genotype and medium as significant. Maeda et al (2002) reported that white and green patches are often seen on the surface stratum of callus with high regeneration ability. Moreover, in sorghum, Kaeppler and Pederson (1997) reported that the production of a high quality callus depends mainly on the genotype.…”

Section: Resultsmentioning

confidence: 99%

Study on Callus Induction System of 4 Genotype of Napiergrass (Pennisetum purpureum )

Umami

Akashi

Gondo

et al. 2016

anprod

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The aim of this study was to produce callus induction potential of 4 napiergrass (Pennisetum purpureum) genotypes (Dwarf Late, Hybrid, Merkeron and Wrukwona). Callus was induced from shoot apices of shoot tillers on MS media containing 2,4-D and BAP. On the MS medium containing 2 mg L–1 2,4-D and 0.5 mg L–1 BAP all genotypes could produce embryogenic calli, with different rate of growth. The best genotype for producing embryogenic calli was dwarf napiergrass in 60 day culture. These genotypes would be usefull for tissue cultured based research and for napiergrass improvement program, particularly in genetic transformation. Culturing shoot apices on MS medium containing 2 mgL–1 2,4-D and 0.5 mgL–1 BAP was more suitable than on MS medium containing 0.5 mgL–1 2,4-D. In the subculture with similar medium composition, proliferation occured poorly on dwarf napiergrass, whereas none happened on the three other genotypes. On the hormon-free medium, all genotypes germinated in different rates. This research pointed out that dwarf napiergrass gave the best response toward induction medium. However, its proliferation and regeneration needed to be optimized in order to obtain more obvious data. This genotype would be usefull for tissue culture based research and for napiergrass improvement program, particularly in genetic transformation.

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Microtopography and Shoot-bud Formation of Rice (Oryza sativa) Callus

Cited by 4 publications

References 61 publications

Tolerance of submerged germinating rice to 50–200 mM NaCl in aerated solution

Tolerance of submerged germinating rice to 50–200 mM NaCl in aerated solution

Identification of Callus Induction Potential of 15 Indonesian Rice Genotypes

Study on Callus Induction System of 4 Genotype of Napiergrass (Pennisetum purpureum )

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