Evaluation of Post-Thaw Sperm Function and Integrity Parameters Under Different Freezing Regimens in Colombian Paso Fino Stallions

Hernández‐Avilés, Camilo; Gómez-Romero, Matthew; Buitrago-Horta, Ricardo; Lozano-Márquez, Harvey; Jiménez-Escobar, Claudia; Zambrano-Varón, Jorge

doi:10.1016/j.jevs.2018.01.008

Cited by 5 publications

(2 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Deterioration of PMI, AI and HIMMP in DMF containing extender compared to glycerol correspond to the motility and viability during the present study. It indicated that HOST and HMMP were the parameters which explained the variability in post-thaw motility observed after freezing (Hernandez-Aviles et al 2018). The HOST predicts the function of the sperm membrane by evaluating the capacity of the sperm cell to react to an osmotic challenge when is incubated in a low-osmolality solution (Ramu and Jeyendran, 2013) and a higher mitochondrial membrane potential is associated with a more efficient energy production through the mitochondrial electron transport chain (Amaral et al 2013).…”

Section: Comparative Evaluation Of Extenders Based On Lipid Per Oxida...mentioning

confidence: 99%

Comparing Glycerol with Dimethylformamide and Combination for Cryopreservation of Pug Dog Semen in Egg Yolk Plasma Containing Extender

Sharma

Cheema

Pathania

2021

IJAR

View full text Add to dashboard Cite

Background: In order to find an alternative cryoprotectant for canine semen, different cryoprotectants, as dimethyl sulphoxide and ethylene glycol, dimethyle formamide either alone or in combination with glycerol have been evaluated on pooled semen of different breeds with different opinions. Methods: In the current study the effect of glycerol, DMF and their combination in Tris-citric acid-fructose egg yolk plasma extender on motility, viability, plasma membrane integrity (PMI), acrosome integrity (AI), inner mitochondrial membrane potential (IMMP), reactive oxygen species (ROS) and antioxidant enzymes in frozen-thaw semen of pug breed was compared. Result: Values for motility, viability, (PMI), AI were significantly (p less than 0.05) high in TCFEYP-G compared to TCFEYP-DMF and TCFEYP-G+DMF at post thaw. However, HIMMP and MIMMP/MDA were non-significantly (P greater than 0.05) high and low in TCFEYP-G compared to TCFEYP-DMF and TCFEYP- G + DMF at post thaw, respectively. MDA concentration was significantly (p less than 0.05) low in TCFEYP-G compared to TCFEYP-DMF and TCFEYP-G + TCFEYP-DMF extenders. However, H2O2, scavenging capacity of spermatozoa, cryopreserved in TCFEYP-G was significantly (p less than 0.05) higher than in TCFEYP-DMF and TCFEYP-G + TCFEYP-DMF. However, there was no significant (p greater than 0.05) difference in superoxide free radical scavenging activity and nitrite concentration of spermatozoa among the three extenders. Activity of GPX, SOD and Catalase was also significantly (p less than 0.05) higher in TCFEYP-G compared to TCFEYP-DMF and TCFEYP-G+DMF. However, values were reverse for GRE activity. This study concludes that glycerol is a healthier cryoprotectant to protect canine spermatozoa from cryo-injury during freezing-thawing process.

show abstract

Section: Comparative Evaluation Of Extenders Based On Lipid Per Oxida...mentioning

confidence: 99%

Comparing Glycerol with Dimethylformamide and Combination for Cryopreservation of Pug Dog Semen in Egg Yolk Plasma Containing Extender

Sharma

Cheema

Pathania

2021

IJAR

View full text Add to dashboard Cite

show abstract

“…é‰ÌËÏ ËÁ ÓÒÌÓ‚Ì˚ı ÔÂËÏÛ˘ÂÒÚ‚ ËÒ-ÔÓÎ¸ÁÓ‚ ‡ÌËfl Á ‡ÏÓÓÊÂÌÌÓÈ ÒÔÂÏ˚ ‚ Î˛•ÓÈ ÔÓ" ‡ÏÏÂ  ‡Á‚Â‰ÂÌËfl ÊË‚ÓÚÌ˚ı fl‚ÎflÂÚÒfl Âfi ‰ÎË-ÚÂÎ¸ÌÓÂ ı ‡ÌÂÌËÂ (‚ ÚÂ˜ÂÌËÂ ÏÌÓ"Ëı ‰ÂÒflÚËÎÂÚËÈ) ‚ ÛÒÎÓ‚Ëflı Ò‚ÂıÌËÁÍËı ÚÂÏÔÂ ‡ÚÛ •ÂÁ ÒÌËÊÂÌËfl Í ‡˜ÂÒÚ‚ÂÌÌ˚ı ı ‡ ‡ÍÚÂËÒÚËÍ, ‡ Ú ‡ÍÊÂ ‚ÓÁÏÓÊ-ÌÓÒÚ¸ Ú ‡ÌÒÔÓÚËÓ‚ÍË ÍËÓÍÓÌÒÂ‚ËÓ‚ ‡ÌÌÓ"Ó "ÂÌÂÚË˜ÂÒÍÓ"Ó Ï ‡ÚÂË ‡Î ‡ Ì ‡ ‰ÎËÚÂÎ¸Ì˚Â  ‡ÒÒÚÓfl-ÌËfl [1,2]. çÂÒÓÏÌÂÌÌÓ, ˜ÚÓ ÔË Á ‡ÏÓ ‡ÊË‚ ‡ÌËË Ë ÔÓÒÎÂ‰Û˛˘ÂÏ ÓÚÚ ‡Ë‚ ‡ÌËË ÒÌËÊ ‡ÂÚÒfl Í ‡˜ÂÒÚ‚Ó ÒÔÂÏ˚, ‡ Ò ‡Ï ‡ ÏÂÚÓ‰ËÍ ‡ ÍËÓÍÓÌÒÂ‚ ‡ˆËË ‰ÓÒÚ ‡-ÚÓ˜ÌÓ ÚÛ‰ÓÂÏÍ ‡fl Ë ˝ÍÓÌÓÏË˜ÂÒÍË Á ‡Ú ‡ÚÌ ‡fl, ÔÓ-˝ÚÓÏÛ ÌÂÓ•ıÓ‰ËÏÓ Ú˘ ‡ÚÂÎ¸ÌÓ ÔÓ‰ıÓ‰ËÚ¸ Í ÓÚ•ÓÛ ÊË‚ÓÚÌ˚ı ‰Îfl ÍËÓÍÓÌÒÂ‚ ‡ˆËË "ÂÌÂÚË˜ÂÒÍÓ"Ó Ï ‡-ÚÂË ‡Î ‡ [3,4]. åÌÓ"ËÂ ËÒÒÎÂ‰Ó‚ ‡ÌËfl Ì ‡Ô ‡‚ÎÂÌ˚ Ì ‡ ÔÓËÒÍ ‰Ë ‡"ÌÓÒÚË˜ÂÒÍËı Ï ‡ÍÂÓ‚, ÍÓÚÓ˚Â ÏÓ"-ÎË •˚ ÔÓÏÓ˜¸ ÒÔÓ"ÌÓÁËÓ‚ ‡Ú¸ Í ‡˜ÂÒÚ‚Ó Ì ‡ÚË‚ÌÓÈ, ÓıÎ ‡Ê‰fiÌÌÓÈ Ë ÍËÓÍÓÌÒÂ‚ËÓ‚ ‡ÌÌÓÈ ÒÔÂÏ˚.…”

unclassified

The level of acute phase blood proteins as a predictive criterion of the cryo resistance of stallion sperm

Atroshchenko

Engalycheva

Shitikova

2022

jour

View full text Add to dashboard Cite

The indicators of the biochemical analysis of the blood of horses reflect the state of metabolism of the animal and at the same time are potential predictors of the qualitative characteristics of spermatozoa, the freezing of which is one of the most important ways to preserve and further use the genetic material. The aim of this study was to identify correlations between the level of such acute phase blood proteins, as C-reactive protein and fibrinogen, and the qualitative and quantitative characteristics of the native and thawed sperm of stallions. The object of the study was blood and sperm samples from 87 stallions of various breeds, the average age of which was 10.5±0.5 years. To assess the protein status of the blood, in addition to the aforementioned acute phase proteins, the total protein content, the levels of albumin, globulins, and the albumin / globulin ratio were determined. Stallion sperm samples were assessed according to the following indicators: ejaculate volume, concentration, the total number of spermatozoa in the ejaculate, progressive motility and survival at +4°C. When analyzing the correlations between the studied parameters, a statistically significant negative inverse relationship was revealed between the level of fibrinogen in the blood serum and such parameters as the content of progressively motile sperm in native and thawed cryopreserved sperm (r=-0.22, p<0.05 and r=-0.29, p<0.01, respectively), as well as sperm survival in native and thawed sperm (r=-0.48, p<0.001 and r=-0.22, p<0.05, respectively).There was no statistically significant relationship between the level of C-reactive protein and the studied characteristics of the spermogram. The revealed negative correlation between the level of fibrinogen and the characteristics of the stallions’ sperm can be considered as one of the prognostic important indicators that must be taken into account when selecting stallions with the highest indicators of the quality of cryopreserved sperm.

show abstract

Effects of egg yolk level, penetrating cryoprotectant, and pre-freeze cooling rate, on the post-thaw quality of stallion sperm

Hernández‐Avilés

Ramírez‐Agámez

Varner

et al. 2023

Animal Reproduction Science

View full text Add to dashboard Cite

Evaluation of Post-Thaw Sperm Function and Integrity Parameters Under Different Freezing Regimens in Colombian Paso Fino Stallions

Cited by 5 publications

References 46 publications

Comparing Glycerol with Dimethylformamide and Combination for Cryopreservation of Pug Dog Semen in Egg Yolk Plasma Containing Extender

Comparing Glycerol with Dimethylformamide and Combination for Cryopreservation of Pug Dog Semen in Egg Yolk Plasma Containing Extender

The level of acute phase blood proteins as a predictive criterion of the cryo resistance of stallion sperm

Effects of egg yolk level, penetrating cryoprotectant, and pre-freeze cooling rate, on the post-thaw quality of stallion sperm

Contact Info

Product

Resources

About