Evaluation of polydimethylsiloxane scaffolds with physiologically-relevant elastic moduli: interplay of substrate mechanics and surface chemistry effects on vascular smooth muscle cell response

Brown, Xin Q.; Ookawa, Keiko; Wong, Joyce Y.

doi:10.1016/j.biomaterials.2004.08.009

Cited by 318 publications

(290 citation statements)

References 19 publications

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“…3A. The material properties such as the elastic modulus of PDMS were assumed to be homogeneous (32). The resulting deformation was not homogeneous across the substrate as expected and experimentally observed because of the pressure and the periphery constraints, but produced a consistent average strain of 10% when deformed.…”

Section: Resultsmentioning

confidence: 95%

Defining the role of syndecan-4 in mechanotransduction using surface-modification approaches

Bellin

Kubicek

Frigault

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

116

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The ability of cells to respond to external mechanical stimulation is a complex and robust process involving a diversity of molecular interactions. Although mechanotransduction has been heavily studied, many questions remain regarding the link between physical stimulation and biochemical response. Of significant interest has been the contribution of the transmembrane proteins involved, and integrins in particular, because of their connectivity to both the extracellular matrix and the cytoskeleton. Here, we demonstrate the existence of a mechanically based initiation molecule, syndecan-4. We first demonstrate the ability of syndecan-4 molecules to support cell attachment and spreading without the direct extracellular binding of integrins. We also examine the distribution of focal adhesion-associated proteins through controlling surface interactions of beads with molecular specificity in binding to living cells. Furthermore, after adhering cells to elastomeric membranes via syndecan-4-specific attachments we mechanically strained the cells via our mechanical stimulation and polymer surface chemical modification approach. We found ERK phosphorylation similar to that shown for mechanotransductive response for integrin-based cell attachments through our elastomeric membrane-based approach and optical magnetic twisting cytometry for syndecan-4. Finally, through the use of cytoskeletal disruption agents, this mechanical signaling was shown to be actin cytoskeleton dependent. We believe that these results will be of interest to a wide range of fields, including mechanotransduction, syndecan biology, and cell-material interactions.T he biochemical response of cells to external mechanical stimulation has generated tremendous interest over the past decade. Of particular interest are the causative contributions of mechanotransductive transmembrane integrin molecules that link the extracellular matrix (ECM) to the cell interior and play a role in physiological response and molecular signaling within the cell. Recently, numerous exciting discoveries have evolved from these studies including the role of mechanotransduction in vascular physiology and atherogenesis, Src activation (Src phosphorylation/ activation under local mechanical stimulation), and the effect of matrix stiffness on stem cell differentiation (1-3). Mechanical stimulation has been shown to produce a wide range of cellular responses including the proteomic activation of the mitogenactivated protein kinase (MAPK) pathways in extracellular signalregulated kinases (ERKs), alterations of genomic expression profiles, and control of cell morphology, differentiation, and proliferation (4, 5). However, such research has focused primarily on the integrins as the mechanical signal initiator/transmembrane protein. Although other strain-sensitive proteins, such as mechanosensitive ion channels and protein kinase C, are known to be altered by mechanical stimulation, these responses are thought to occur downstream or be directly linked to the transmembrane proteininitiating...

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Section: Resultsmentioning

confidence: 95%

Defining the role of syndecan-4 in mechanotransduction using surface-modification approaches

Bellin

Kubicek

Frigault

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

116

View full text Add to dashboard Cite

show abstract

“…44 The deposits of LbL films that are made of synthetic or natural polymers have recently appeared as an interesting strategy that is used to promote cell attachment on PDMS. 32,47 In our study, a parallel orientation of myoblasts and myotubes was obtained with microstructured SilPEM. We showed here that myoblasts already began to orient and to develop cell extensions following the linear orientation of the pattern after *15 h in GM (Fig.…”

Section: Filmsmentioning

confidence: 99%

“…Earlier, it was shown that the functionalization of PDMS is needed to support the adhesion and vascular smooth muscle cells. 32 For C2C12 cells, previous studies used PDMS in combination with a coating of an ECM protein, either laminin 9,11 or collagen. 33 However, batch-tobatch quality of the protein and initial state of the PDMS substrate may lead to poor reproducibility in the results between different research groups.…”

Section: Filmsmentioning

confidence: 99%

Engineering Muscle Tissues on Microstructured Polyelectrolyte Multilayer Films

Monge

Ren

Berton

et al. 2012

Tissue Engineering Part A

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“…By comparison, PDMS has a tensile Young's modulus in the range of 1.32-2.97 MPa, slightly higher than that of skin [33][34][35]. It is proved that compatible component can endow the polymer blend improved mechanical properties [36].…”

Section: Resultsmentioning

confidence: 96%