Evaluation of Pluronic Polyols as Carriers for Grafting Materials: Study in Rat Calvaria Defects

Fowler, Edward B.; Cuenin, Michael F.; Hokett, Steven D.; Peacock, Mark E.; McPherson, James C.; Dirksen, Thomas R.; Sharawy, Mohamed; Billman, Michael A.

doi:10.1902/jop.2002.73.2.191

Cited by 47 publications

(44 citation statements)

References 22 publications

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“…23 F-127 also enhances early fibroblast attachment and collagen formation that augments the angiogenesis in microcirculation, 18 increasing blood supply to the site of new bone formation. 12 A critical variable in cell-based grafting techniques is the number of cells used for implantation. The use of a larger volume of bone marrow improves bone remodeling and formation, and results in a higher rate of bone union.…”

Section: Discussionmentioning

confidence: 99%

“…This property makes Pluronic F127 useful for introducing and maintaining a graft at the intended site. 9,12 b-Tricalcium phosphate (b-TCP) and hydroxyapatite (HA), two calcium phosphate ceramics, have been used as biomaterials to construct bone graft substitutes for treating bone defects. 13,14 Interpore, a coralline HA, is an absorbable porous ceramic, which has been used clinically in orthopedic and craniofacial surgeries.…”

mentioning

confidence: 99%

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Use of fluorescence labeled mesenchymal stem cells in pluronic F127 and porous hydroxyapatite as a bone substitute for posterolateral spinal fusion

Chen

Huang

Niu

et al. 2009

Journal Orthopaedic Research

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Posterolateral spinal fusion is used to treat patients with degenerative spinal disorders. We investigated the effectiveness of a mesenchymal stem cell (MSC)/Pluronic F127/Interpore hybrid graft for spinal fusion in rabbits. Spinal fusion was examined using radiography, manual palpation, computed tomography (CT), torsional loading tests, and histological analysis. Using a PKH fluorescence labeling system, we also examined whether the newly formed bone was derived from the transplanted MSCs. We found that the MSCs adhered to the Interpore surface and within its pores, and differentiated into osteoblasts. Radiographs and CT images showed a continuous bone bridge and a satisfactory fusion mass incorporated into the transverse processes. The results of manual palpation and biomechanical data did not differ significantly from an autograft group. Histology from both groups revealed the presence of fibrous tissue, cartilage, and endochondral ossification in the gaps between the grafted fragments. In both groups, the degree of mature bone formation was greater at 12 weeks than at 6 weeks after grafting. Quantitative histomorphometry revealed no significant differences between the two groups at either time point. In situ tracing of the PKH 67-labeled MSCs indicated that the transplanted MSCs were partly responsible for the new bone formation in both the repaired transverse processes and the grafted fragments. Thus, the MSC/Pluronic F127/Interpore hybrid graft could be used effectively to achieve posterolateral spinal fusion. ß

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Use of fluorescence labeled mesenchymal stem cells in pluronic F127 and porous hydroxyapatite as a bone substitute for posterolateral spinal fusion

Chen

Huang

Niu

et al. 2009

Journal Orthopaedic Research

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“…Poloxamer has been proposed as a carrier for osseous graft material either topically or systemically (113,114). It facilitates early collagen synthesis and microcirculation.…”

Section: Promotion Of Cell Multiplicationmentioning

confidence: 99%

“…It facilitates early collagen synthesis and microcirculation. A Poloxamer 407 hydrogel enveloping a non-biodegradable endoskeletal scaffold shows interesting prospects to generate tissue of intricate shape (e.g., human ear) (113,115). Very low concentrations of Poloxamer 188 and Poloxamer 407 increase attachment and growth of human gingival fibroblasts and may have applications in early post surgical wound healing (116).…”

Section: Promotion Of Cell Multiplicationmentioning

confidence: 99%

A Review of Poloxamer 407 Pharmaceutical and Pharmacological Characteristics

et al. 2006

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Poloxamer 407 copolymer (ethylene oxide and propylene oxide blocks) shows thermoreversible properties, which is of the utmost interest in optimising drug formulation (fluid state at room temperature facilitating administration and gel state above sol-gel transition temperature at body temperature promoting prolonged release of pharmacological agents). Pharmaceutical evaluation consists in determining the rheological behaviour (flow curve or oscillatory studies), sol-gel transition temperature, in vitro drug release using either synthetic or physiological membrane and (bio)adhesion characteristics. Poloxamer 407 formulations led to enhanced solubilisation of poorly water-soluble drugs and prolonged release profile for many galenic applications (e.g., oral, rectal, topical, ophthalmic, nasal and injectable preparations) but did not clearly show any relevant advantages when used alone. Combination with other excipients like Poloxamer 188 or mucoadhesive polymers promotes Poloxamer 407 action by optimising sol-gel transition temperature or increasing bioadhesive properties. Inclusion of liposomes or micro(nano)particles in Poloxamer 407 formulations offers interesting prospects, as well. Besides these promising data, Poloxamer 407 has been held responsible for lipidic profile alteration and possible renal toxicity, which compromises its development for parenteral applications. In addition, new findings have demonstrated immuno-modulation and cytotoxicity-promoting properties of Poloxamer 407 revealing significant pharmacological interest and, hence, human trials are in progress to specify these potential applications.

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“…21,22 It has been suggested that PF127 can enhance early wound healing in third-degree burns; it has also been considered as a carrier for osseous graft materials and growth factors. 25 PF127 facilitated the spread, attachment and growth of human gingival fibroblasts. 20 PF127 increased transgene expression and is a suitable carrier for regional gene therapy.…”

Section: Discussionmentioning

confidence: 96%

Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene: an alternative to alveolaplasty

Chen

Jeng

et al. 2008

Gene Ther

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Evaluation of Pluronic Polyols as Carriers for Grafting Materials: Study in Rat Calvaria Defects

Cited by 47 publications

References 22 publications

Use of fluorescence labeled mesenchymal stem cells in pluronic F127 and porous hydroxyapatite as a bone substitute for posterolateral spinal fusion

Use of fluorescence labeled mesenchymal stem cells in pluronic F127 and porous hydroxyapatite as a bone substitute for posterolateral spinal fusion

A Review of Poloxamer 407 Pharmaceutical and Pharmacological Characteristics

Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene: an alternative to alveolaplasty

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