Evaluation of platelet activation and cytokine release during storage of platelet concentrates processed from buffy coats either manually or by the automated OrbiSac system

Vetlesen, Annette; Mirlashari, Mohammad Reza; Ezligini, Farshid; Kjeldsen‐Kragh, Jens

doi:10.1111/j.1537-2995.2007.01075.x

Cited by 32 publications

(33 citation statements)

References 18 publications

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“…Further, by gating on the CD41a‐positive events, PLTs were identifiable, even if aggregated upon TRAP‐6 activation. Our data are in line with previous reports showing an increase of P‐selectin expression during storage 1,22 . Whether or not this increase of P‐selectin is detrimental to PLT function is disputed 23,24 .…”

Section: Discussionsupporting

confidence: 93%

Platelet function under high‐shear conditions from platelet concentrates

et al. 2007

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BACKGROUND: Platelet (PLT) collection and storage affect the functional capacity of PLTs in PLT concentrates (PCs). Therefore, PLTs' functional quality should be studied before transfusion. STUDY DESIGN AND METHODS: PCs (n = 15) were collected by a standard apheresis procedure (Trima, Gambro BCT) and were stored for 7 days. Samples were taken to assess PLT adhesion and aggregate formation by a cone and plate analyzer (Impact-R, DiaMed) on Days 1, 3, 5, and 7 after harvesting. This device allows testing PLT function under high-shear stress close to physiologic conditions. Concomitantly, P-selectin expression and the residual responsiveness to TRAP-6 were determined by flow cytometry. RESULTS: PLT adhesion, as measured by surface coverage, decreased during the entire observation period; likewise, the size of aggregates was significantly lower on Days 5 and 7 compared to Day 1 (p < 0.02). P-selectin expression increased from Day 5 to Day 7 (p < 0.04), whereas TRAP-6-inducible expression remained stable until Day 5 of storage and decreased significantly on Day 7 (p = 0.04). CONCLUSIONS: Our results show that high-shearinduced PLT adhesion and aggregation on the polystyrene surface deteriorate upon storage, suggesting decreased PLT function in vivo. Thus, the Impact-R may be a useful tool to assess the functional capacity of PLTs under various PLT harvesting and storage procedures. P latelet (PLT) transfusions are used to prevent or treat bleeding episodes in patients with thrombocytopenia, for example, after high-dose chemotherapy. The process of PLT collection and storage may affect the functional capacity of PLTs in PLT concentrates (PCs). Therefore, a variety of in vitro methods have been extensively investigated for their validity and feasibility to test and guarantee the function of PLTs that were prepared for their transfusion.1 Results from in vitro tests were correlated with the corrected count increment (CCI) of PLTs 1 or 24 hours after transfusion to estimate the validity of these tests clinically. 2-4Although the CCI is most often used to estimate the success of PLT transfusions, it does not always correlate with PLT survival.1 Very few in vitro tests performed on PLTs from PCs, however, correlate with PLT viability after their transfusion to healthy individuals, 4,5 and these tests have not been further validated after transfusion to thrombocytopenic patients. Further, the CCI does not evaluate PLTs' hemostatic activity. Thus, in vitro evaluation of PLT function in PCs is desirable before transfusion. According to the European guidelines, only the pH must be measured (6.4-7.4) and the swirling phenomenon must be demonstrated after 5 days of storage. This device allows evaluation of PLT function under close to physiologic conditions in a whole-blood assay. PLT adhesion and aggregation can be determined in anticoagulated blood under arterial flow conditions wherein a cone and plate viscometer induces laminar flow with a uniform shear stress over a plastic plate by the rotating cone. Results can then be e...

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Section: Discussionsupporting

confidence: 93%

Platelet function under high‐shear conditions from platelet concentrates

et al. 2007

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“…However, the extent of platelet activation in freshly drawn donor blood is not likely to be as high as the maximum inducible in vitro. Of relevance, during storage of platelet concentrates, the surface activation markers CD62P and PAC-1 increase up to 600 and 150%, respectively, without any significant change in MPV [36], belying an effect, if any, of platelet activation on MPV.…”

Section: Risk Factors For Thrombosismentioning

confidence: 97%

Genetic variants that affect platelet function

Kunicki

Williams

Nugent

2012

Current Opinion in Hematology

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Variation in MPV between normal individuals is responsible for roughly a two-fold range in platelet protein content, including key surface receptors and reactive granule constituents, the association of ADRA2, GP1BA, GP6, ITGA2 and P2Y12 variants with platelet reactivity, initially identified by candidate gene analyses, has now been validated by genome-wide approaches in much larger individual cohorts, and GWAS have identified novel gene variants, most notably PEAR1, that participate in variation in platelet reactivity among normal individuals, all of which contribute to a genetic basis for differences in platelet reactivty among normal individuals.

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“…CD62P, also known as platelet α-granule membrane protein-140 (GMP-140), is expressed on the platelet plasma membrane during platelet activation, but not on the resting platelet. CD62P can reportedly transfer rapidly to the platelet surface when the platelet is activated (Ritchie et al, 2000;Vetlesen et al, 2007). Among the surface-activated markers of platelets, CD62P expression shows the most significant difference between activated and non-activated platelets.…”

Section: Discussionmentioning

confidence: 99%

Effect of leukocyte filtration on the P-selectin expression of apheresis platelets

Chen

Zhang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to investigate the effect of leukocyte filtration on the P-selectin (CD62P) surface expression of apheresis platelets during the retention period. Ten bags of apheresis platelets stored for 1 day (0-24 h) and 10 bags of apheresis platelets stored for 2 days (24-48 h) were used for leukocyte filtration (experimental group). Ten bags of apheresis platelets with the corresponding retention periods but without filtration were used as a negative control (control group). Thereafter, 100 μL of platelet suspensions from apheresis platelets with or without leukocyte filtration were sampled before and after leukocyte filtration for the detection of CD62P surface expression by flow cytometry. No statistical difference in the CD62P (2015) surface expression of apheresis platelets was observed before and after leukocyte filtration (P > 0.05), neither did the CD62P surface expression exhibit any change among the different retention periods. Leukocyte filtration does not affect the CD62P surface expression of apheresis platelets stored for up to 2 days, which indicates that leukocyte filtration does not damage the activation of apheresis platelets within the retention period.

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Evaluation of platelet activation and cytokine release during storage of platelet concentrates processed from buffy coats either manually or by the automated OrbiSac system

Abstract: The levels of CCL5 and the expression of CD62P in resting and stimulated PLTs indicate that PLTs in A-PCs are slightly more activated than in M-PCs, but the clinical importance of this finding is yet unknown.

Cited by 32 publications

References 18 publications

Platelet function under high‐shear conditions from platelet concentrates

Platelet function under high‐shear conditions from platelet concentrates

Genetic variants that affect platelet function

Effect of leukocyte filtration on the P-selectin expression of apheresis platelets

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