Evaluation of plant regeneration ability of different explants and establishment of an efficient regeneration system using immature seeds in Actinidia kolomikta , a cold-hardy kiwifruit relative

Asakura, Issei; Hoshino, Yoichiro

doi:10.1016/j.scienta.2017.04.012

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…The ability of isolated endosperm tissue to proliferate and regenerate has been proved for a wide range of species (Hoshino et al 2011;Wang et al 2016;Winarto et al 2018) including the genus Actinidia, like A. deliciosa (Goralski et al 2005) or A. kolomikta (Asakura and Hoshino 2017). The experimental system that had previously been established for obtaining the endosperm-derived callus in A. deliciosa (Goralski et al 2005) was also found to be appropriate for A. arguta (not published).…”

Section: General Ultrastructure and Histology Of A Callus With A Divementioning

confidence: 99%

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Popielarska-Konieczna

Sala

Abdullah

et al. 2020

Plant Cell Rep

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Key message Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit. Abstract The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue. In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta. The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway. The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli. The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures. The non-organogenic tissue was composed of loosely attached cells, which were connected via a net-like structure. The extracellular matrices from both the non-and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus. Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).

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Section: General Ultrastructure and Histology Of A Callus With A Divementioning

confidence: 99%

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Popielarska-Konieczna

Sala

Abdullah

et al. 2020

Plant Cell Rep

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“…Plant tissue culture has a wide range of uses in modern biotechnology. The regeneration abilities of different parts of explants are different [ 10 , 27 , 28 ]. We found that a large number of calli formed at the base of long shoot tips, but these calli were unable to regenerate.…”

Section: Discussionmentioning

confidence: 99%

Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses

Dong

Zhang

et al. 2022

Plants

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Shoot tip culture is a very effective approach for studying plant viruses. In this study, we evaluated the numbers, diversity, and titer of grapevine viruses in in vitro grapevine plants after long shoot tip culture. Six virus-infected grapevine cultivars (Cabernet Franc, Cabernet Gernischt, Cabernet Sauvignon, Wink, Victoria, and Merlot) collected from six regions of China were used as the research materials. Approximately 1.5 cm long shoot tips were used for meristem culture. The average survival rate of the six grapevine cultivars was 45.7%. Merlot collected from Beijing showed the highest survival rate (80.0%). Regeneration was not achieved in Cabernet Gernischt collected from Liaoning province and Cabernet Sauvignon from Tianjin due to bacterial and fungal contamination. Virus detection conducted in the surviving regenerated plants showed that the virus infection status, including the viral numbers and the species present in plants grown in vitro, was the same as that in corresponding in vivo plants. Moreover, the analysis of sequence diversity and the mutation frequency in grapevine viruses in vitro indicated that the structure of grapevine viruses was stable in long shoot tip culture after four sub-culture passages. Further, the relative viral titer of in vitro grapevine plants was much higher than that of in vivo plants. These results aid in the investigation of viruses in woody plants.

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“…Previous research showed that when one callus was transferred to regeneration mediums containing a reduced amount of 2,4-D and a high content of kinetin, green spots were detected easily (Abe & Futsuhara, 1986). Similarly, a large number of buds can be obtained by culturing on MS medium containing 2.0 mg L -1 2,4-D and 5.0 mg L -1 KT (Asakura & Hoshino, 2017). Wu et al (2019) studied the factors affecting callus induction from lily (Lilium spp.)…”

Section: Effects Of 24-d and Kt On Callus Inductions At The Different Concentrationsmentioning

confidence: 99%

Efficient callus induction and regeneration in proso millet

Liu

Yang

et al. 2021

Agronomy Journal

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Proso millet (Panicum miliaceum L.) is an important drought-tolerant crop, and recently it has become a research focus because of its health-care and nutrition significance. A study was carried out to investigate callus induction and differentiation and plantlet rooting in Shaanmi 1 and Shaanmi 2 with young leaves of the two varieties. Its result showed that in proso millet, the age of aseptic seedlings could influence on inducing calli from young leaves with the highest callus induction frequency at the seedling age of 7 d. N6 medium performed better in inducing calli from young leaves. There did appear to be no calli on MS medium without 2,4-D, but calli were induced on N6 medium without 2,4-D in Shaanmi 2, with its induction frequency standing 6.45% and no calli were induced on the medium without 2,4-D in Shaanmi 1. With 2,4-D added, there appeared the highest callus induction frequency at a 2,4-D concentration of 3.0 mg L -1 . The optimal medium to induce calli from young leaves in proso millet was N6 medium plus 2,4-D (3.0 mg L -1 ) and KT (0.5 mg L -1 ), with its callus induction frequency at 64.33% (Shaanmi 1) and 68.67% (Shaanmi 2), respectively. The optimal callus differentiation medium was MS medium plus 1.0 mg L -1 6-BA and 0.5 mg L -1 NAA, with its differentiation rate at 87.78% (Shaanmi 1) and 96.29% (Shaanmi 2), respectively. Proline adding at 600 mg L -1 was conducive to callus differentiation. The optimal rooting medium was 1/2MS medium plus NAA (0.1 mg L -1 ), with its rooting rate at 100%.

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Evaluation of plant regeneration ability of different explants and establishment of an efficient regeneration system using immature seeds in Actinidia kolomikta , a cold-hardy kiwifruit relative

Cited by 8 publications

References 18 publications

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta

Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses

Efficient callus induction and regeneration in proso millet

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