Evaluation of phytotoxicity, cytotoxicity, and genotoxicity of ZnO nanoparticles in Vicia faba

Youssef, Mohamed S.; Elamawi, Rabab M.

doi:10.1007/s11356-018-3250-1

Cited by 73 publications

(31 citation statements)

References 54 publications

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“…Finally, the mycogenic AgNPs showed antimicrobial activities with efficiencies comparable to the positive controls, especially toward Gentamicin and Amoxicillin for pathogenic bacteria and Clotrimazole and Nystatin for pathogenic fungi. Many reports discuss the promising approach of AgNPs for the development of novel antimicrobial compounds [68][69][70][71][72]. The findings from our antimicrobial studies, which are in accordance with the findings of many authors, confirm the possibility of achieving a higher inhibition rate toward a broad range of pathogenic microbes, even with a lower concentration [73][74][75].…”

Section: Resultssupporting

confidence: 91%

Mycogenic Silver Nanoparticles from Endophytic Trichoderma atroviride with Antimicrobial Activity

Abdel-Azeem

Nada

O’Donovan

et al. 2020

Journal of Renewable Materials

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There is an increasing interest in developing nanoparticles with diverse biologic activities. To this end, we prepared 10 to 15 nm silver nanoparticles (AgNP) from native isolates of Trichoderma atroviride. Within this study, endophytic fungi hosted four medicinal plants in Saint Katherine Protectorate, South Sinai, Egypt have been isolated by surface sterilization technique on four isolation media. Ten species, based on their frequency of occurrence, out of twenty recovered taxa were tested for their capability to synthesize extracellular AgNPs. Trichoderma atroviride hosted Chiliadenus montanus was found to be the best candidate for the production of mycogenic AgNPs among all examined species. The mycosynthesized AgNPs were compared with chemically synthesized and characterized using Ultraviolet-visible (UV-vis) spectroscopy, Raman spectroscopy, X-ray diffraction (XRD) and high-resolution transmission electron microscopy (HRTEM) techniques. The HRTEM result showed the distribution of spherical AgNPs ranging from 10 to 15 nm. Trichoderma atroviride isolate was subjected to sequencing for confirmation of phenotypic identification. The internal transcribed spacer (ITS) 1-5.8 s-ITS2 rDNA sequences obtained were compared with those deposited in the GenBank Database and registered with accession number MH283876 in the NCBI Database. Antibacterial, anticandidal and antifungal effects of chemically and mycosynthesized AgNPs were examined at various concentrations in vitro against six pathogenic bacteria and 4 pathogenic fungi by agar well diffusion technique. Standard antibiotics; Gentamicin, Amoxicillin, Clotrimazole, and Nystatin at 5 μg/disk were taken as positive controls, while 5% DMSO was used as the negative control. Our data revealed that the application of mycogenic AgNPs at a concentration of 100 ppm resulted in maximum inhibition of pathogenic bacteria and fungi. These data suggest that AgNPs from native isolates of Trichoderma atroviride (MH283876) offer a source of rapid synthesis of eco-friendly, economical biomaterials that show antimicrobial activities.

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Section: Resultssupporting

confidence: 91%

Mycogenic Silver Nanoparticles from Endophytic Trichoderma atroviride with Antimicrobial Activity

Abdel-Azeem

Nada

O’Donovan

et al. 2020

Journal of Renewable Materials

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“…Nanotoxicity of ZnO NPs to plants at the phytotoxicity and genotoxic level has been demonstrated by seed germination, seedling growth, isoenzyme systems, cytogenetic test (Youssef and Elamawi 2018). The results presented herein showed that, the effect of ZnO-NPs on germination was not varied significantly between different concentrations and the control.…”

Section: Discussionmentioning

confidence: 65%

“…The ZnO nanopowder (M K Impex Corp Mississauga, Canada) with primary diameter of 30 nm was used to prepare five different concentrations of ZnO NPs (10, 25, 50, 100, and 200 mg/L). The preparation and characterization of ZnO NPs suspension were fully described in previous paper (Youssef and Elamawi 2018).…”

Section: Preparation Of Zno Nanoparticlementioning

confidence: 99%

“…Cyamopsis tetragonoloba when exposed to ZnO NPs, showed improved plant biomass, root and shoot length, chlorophyll and protein synthesis and other growth parameters (Raliya and Tarafdar 2013). Lower concentrations of ZnO NPs (10 and 25 mg/L) enhanced seed germination and improved seedling growth, while higher concentrations (100 and 200 mg/L) resulted in phytotoxicity in the Vicia faba (Youssef and Elamawi 2018).…”

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confidence: 99%

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Estimate of the Antifungal Activity and Phytotoxicity of ZnO Nanoparticles on Magnaporthe oryzae and Rice Cultivar Sakha 101 using Morphological, Biochemical and Molecular Markers

Elamawi

Youssef

El–Refaee³

2019

Egyptian Journal of Phytopathology

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ice blast caused by Magnaporthe oryzae is the major biotic stress influences rice yield. The current investigation aimed to estimate the antifungal activity and phytotoxicity effect of different concentrations of ZnO nanoparticles (0.0, 10, 25, 50, 100 and 200 mg/L) on M. oryzae and rice cultivar Sakha101 using morphological, biochemical and molecular markers. Five ISSR markers and seven RAPD primers were utilized to estimate the potentiality effects of ZnO nanoparticles (NPs). The effect of different rates and applications of ZnO NPs used to control rice blast disease and improve grain yield were estimated in the field during 2017 and 2018 growing seasons. The results showed that, in vitro antifungal assay of ZnO NPs showed a significant decrease in colony formation in comparison to control. Foliar application of ZnO NPs at 25 mg/L was the most effective treatments for mitigation rice blast at five days before inoculation. While under field conditions, foliar spray with 25 mg/L at nursery increased the grain yield to 4.366 ton/fed in season 2017 and to 4.625 ton/fed in 2018. Foliar spray with ZnO NPs of rice offer a practical and useful approach to improve rice grain yield and reduce leaf blast disease when applied at optimal concentrations. ZnO NPs at lower concentrations (10 and 25 mg/L) enhanced seed germination and improved seedling growth, while the higher concentrations (100 and 200 mg/L) resulted in phytotoxicity. ZnO NPs treatments altered the expression patterns of seeds storage protein; induced newly synthesized isoforms and disappearance of existing ones. The obtained results using molecular markers confirmed that the lower concentrations of ZnO-NPs (10 and 25 mg/L) are considered as a good enhancement agent, as in case of rice cultivar Sakha101, using UBC 825 primer with 306 bp at concentration of 25 mg/L and OPA-9 primer with 948 bp at concentration of 10 mg/L.

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“…Zinc oxide nanoparticles in an aqueous solution under UV radiation have a phototoxic effect based on the production of H 2 O 2 and O 2− reactive oxygen species. Adverse effects of ZnO NPs have been demonstrated on several plant species including pea, corn, cucumber, rye, zucchini, soybean, wheat, Fagopyrum esculentum , Arabidopsis thaliana , Vicia faba , and Salicornia [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. Biological effects of ZnO NPs depend on different factors, such as particle size, morphology, surface modification, photocatalytic activity, concentration, plant species, and growth conditions [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

2020

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Nanomaterials, including zinc oxide nanoparticles (ZnO NPs), have a great application potential in many fields, such as medicine, the textile industry, electronics, and cosmetics. Their impact on the environment must be carefully investigated and specified due to their wide range of application. However, the amount of data on possible negative effects of ZnO NPs on plants at the cellular level are still insufficient. Thus, we focused on the effect of ZnO NPs on tobacco BY-2 cells, i.e., a widely accepted plant cell model. Adverse effects of ZnO NPs on both growth and biochemical parameters were observed. In addition, reactive oxygen and nitrogen species visualizations confirmed that ZnO NPs may induce oxidative stress. All these changes were associated with the lipid peroxidation and changes in the plasma membrane integrity, which together with endoplasmatic reticulum and mitochondrial dysfunction led to autophagy and programmed cell death. The present study demonstrates that the phytotoxic effect of ZnO NPs on the BY-2 cells is very complex and needs further investigation.

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Evaluation of phytotoxicity, cytotoxicity, and genotoxicity of ZnO nanoparticles in Vicia faba

Cited by 73 publications

References 54 publications

Mycogenic Silver Nanoparticles from Endophytic Trichoderma atroviride with Antimicrobial Activity

Mycogenic Silver Nanoparticles from Endophytic Trichoderma atroviride with Antimicrobial Activity

Estimate of the Antifungal Activity and Phytotoxicity of ZnO Nanoparticles on Magnaporthe oryzae and Rice Cultivar Sakha 101 using Morphological, Biochemical and Molecular Markers

Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

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