Estimate of the Antifungal Activity and Phytotoxicity of ZnO Nanoparticles on Magnaporthe oryzae and Rice Cultivar Sakha 101 using Morphological, Biochemical and Molecular Markers

Elamawi, Rabab M.; Youssef, Mohamed S.; El–Refaee, Y. Z.

doi:10.21608/ejp.2019.119996

Cited by 3 publications

(1 citation statement)

References 30 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the case of the rice cultivars Giza 177, Sakha super 300, and Sakha 108, using seven ISSR primers and three RAPD primers with 24 unique bands, the obtained results using molecular markers confirmed that the lower concentrations of ZnO-NPs and MgO nanoparticles (10 and 20 mg/L) are considered to be a good enhancement agent. These findings generally indicated that lower ZnO and MgO NP concentrations might be used as an efficient nanofertilizer for sustainable agriculture and food safety, as well as an antifungal agent for the rice fungus Fusarium moniliforme (Elamawi et al, 2019).…”

Section: Genetic Diversity and Relationshipsmentioning

confidence: 80%

Detected Genetic Markers for Three Varieties of Rice (Oryza sativa L.) under Nano- Particles

El-Abeid,

Heiba,

El-Demerdash

et al. 2023

J. Adv. Zool.

View full text Add to dashboard Cite

Despite the enormous advancements in breeding, fresh and accessible methods of plant development are preferred. Many research have been done to determine the impact of zinc oxide nanoparticles (ZnO NPs) and magnesium nanoparticles (MgONPs) on plant growth. ZnO NPs and MgONPs have been used extensively in agriculture. Few research have looked into the potential impact of ZnO NPs on grain Zn content or cereal yield formation to date. The goal of the study was to demonstrate how varied concentrations of nanoparticles affected root germination in three different rice types (Giza 177, Sakha super 300, and Sakha 108) and the fungus Fusariummoniliforme, which causes foot rot in rice. Here, we performed a pot experiment to assess the effects that ZnO nanoparticles and MgONPs had on the shoot length, root length, and germination of rice. The experiment involved three dosages of Zn and Mg nanoparticle solution at varying concentrations of 10, 20, and 100 ppm. The outcomes showed that, when compared to the control treatment, ZnO NPs and MgONPs increased the length of the shoot, the length of the roots, and the rate of germination. Using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers, the genetic makeup of plants treated with 10, 20, and 100 ppm AgNPs was also examined. In vitro rooted shoots were acclimated in a greenhouse before being tested for biochemical stability and phenotypic stability. In vitro, callogenesis and caulogenesis were both reduced by AgNPs at the greatest dose (100 ppm). In order to maximise genome coverage, three rice cultivars from distinct origins were chosen, and they were tested using three RAPD markers and seven selected ISSR markers. AgNPs-treated plants using ISSR markers produced 87 amplified bands in total, 45 polymorphic allelic variants, and 36 monomorphic allelic variations. The Pearson correlation coefficient was used in the Mantel test to determine whether there was a significant association between the Jaccard's dissimilarity matrices based on ISSR and RAPD markers (r = 0.69; P 0.05). Morphological characteristics, ISSR, and RAPD analysis were used to partition the UPGMA Dendrogram into two groupings. Jaccard's coefficient was used to analyse the genetic similarity matrix. Sakha Super 300 and Giza 177 had the most genetic similarity (95%) while Sakha 108 and Giza 177 had the lowest genetic similarity (90%) according to ISSR and RAPD study. The classification of rice germplasm, breeding initiatives, and conservation efforts all heavily rely on the determination of genetic diversity within the rice species. To find genetic variations, morphological characteristics, ISSR, and RAPD analysis are useful techniques. The findings demonstrated the large ratio of variation in rice. This work successfully established the possibility of using MgONPs and ZnO NPs as high-performing fertilisers to improve rice output and quality.This study could serve as a guide for future research on rice and could support efforts to understand the species and improve breeding stock.

show abstract

Section: Genetic Diversity and Relationshipsmentioning

confidence: 80%

Detected Genetic Markers for Three Varieties of Rice (Oryza sativa L.) under Nano- Particles

El-Abeid,

Heiba,

El-Demerdash

et al. 2023

J. Adv. Zool.

View full text Add to dashboard Cite

show abstract

The Effect of Incorporated Aloe vera Extracts onto Zeolitic Imidazolate Framework on Physiological, Biochemical, and Molecular Behavior of Quinoa (Chenopodium quinoa Willd.) Genotype Under Saline Conditions

Mahdi

Mahmoud

Abdelhameed

2021

J Soil Sci Plant Nutr

View full text Add to dashboard Cite

Antifungal properties and phytotoxicity of ZnO nanoparticles – a genotypic dependent effect

Tang,

Yeo,

Chin

et al. 2023

Archives of Phytopathology and Plant Protection

View full text Add to dashboard Cite

Pyricularia oryzae, the causal agent of rice blast disease, is a serious threat to rice. it reduces the yield and quality of rice. agrofungicide used to control rice blast is harming the environment and human health. safer alternative is needed. this study aimed to investigate the antifungal activity of zinc oxide nanoparticles (ZnO NPs) against P. oryzae of sarawak, and its phytotoxicity towards sarawak rice. solvothermal sysnthesis method was used to synthesize two sizes of ZnO NPs (~20 nm and ~40 nm). ZnO NPs showed inhibition effect on P. oryzae tested and the effect was isolate dependent. the effect increased along with the concentration increment of ZnO NPs. ZnO NPs were found not affecting the seed germination rate, but on the shoot and root development. the effect varies at different concentration treatments of ZnO NPs. landrace/genotype dependent phytotoxicity effect of ZnO NPs was observed.

show abstract

Estimate of the Antifungal Activity and Phytotoxicity of ZnO Nanoparticles on Magnaporthe oryzae and Rice Cultivar Sakha 101 using Morphological, Biochemical and Molecular Markers

Cited by 3 publications

References 30 publications

Detected Genetic Markers for Three Varieties of Rice (Oryza sativa L.) under Nano- Particles

Detected Genetic Markers for Three Varieties of Rice (Oryza sativa L.) under Nano- Particles

The Effect of Incorporated Aloe vera Extracts onto Zeolitic Imidazolate Framework on Physiological, Biochemical, and Molecular Behavior of Quinoa (Chenopodium quinoa Willd.) Genotype Under Saline Conditions

Antifungal properties and phytotoxicity of ZnO nanoparticles – a genotypic dependent effect

Contact Info

Product

Resources

About