Evaluation of photo-activated disinfection effectiveness with methylene blue against Porphyromonas gingivalis involved in endodontic infection: An in vitro study

Pourhajibagher, Maryam; Chiniforush, Nasim; Raoofian, Reza; Pourakbari, Babak; Ghorbanzadeh, Roghayeh; Bazarjani, Farzaneh; Bahador, Abbas

doi:10.1016/j.pdpdt.2016.09.008

Cited by 29 publications

(11 citation statements)

References 15 publications

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“…The findings of this study showed that MB-sPAD (25 μg/ml-117.18 J/cm 2 ) and (50-100 μg/mL-117.18 J/cm 2 ) significantly inhibited the growth of P. gingivalis than the control group. 34 Boluki et al studied the effect of photodynamic therapy (TBO with 630 nm LED laser light) with colistin on A. baumannii pandrug resistant strains. The results of this study showed that the effect of TBO+LED significantly reduced the growth of bacterium.…”

Section: Application Of Photodynamic Therapy In Vitromentioning

confidence: 99%

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

et al. 2018

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Introduction: The purpose of this review was to evaluate the available literature for in vitro and in vivo effectiveness of antimicrobial Photodynamic therapy (aPDT) in the field of bacteriology. Methods: A review of the relevant articles carried out in PubMed and Scopus to determine the efficiency of aPDT used in the reduction of microbial infection. Thirty-one relevant documents retrieved from PubMed, Scopus by inserting "antimicrobial photodynamic therapy" and "bacterial infection" and "photodynamic therapy" keywords. Results: According to different results, aPDT can be used as an adjuvant for the treatment of infectious diseases. The use of photosensitizer methylene blue, toluidine blue O (TBO), indocyanine green with light diode laser centered at (630±10 nm) and (650±10 nm) wavelengths have been shown to have significant results for the treatment of infectious diseases and bactericidal properties Conclusion: These findings suggest that, aPDT can be an efficient method in the treatment of localized and superficial infections.

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Section: Application Of Photodynamic Therapy In Vitromentioning

confidence: 99%

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

et al. 2018

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“…14,15,16 All the wells of round-bottom 96-well microtiter plates (TPP, Trasadingen, Switzerland) were filled with 90 microliter (µL) brain heart infusion (BHI) broth (Merck, Darmstadt, Germany). Next, 10 μL of each sample was transferred to the wells in column 1 and was diluted ten-fold stepwise from column 1 to column 5.…”

Section: Culture Analysis Of the Microbiotamentioning

confidence: 99%

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Pourhajibagher

Ghorbanzadeh²,

Bahador

2017

Braz. oral res.

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Endodontic infections are considered to be caused by the presence of various microorganisms within the root canal system. Recognition of this microbiota contributes to the successful treatment of infected root canals. This study investigated the microorganisms associated with primary and secondary endodontic infections via culture methods, biochemical tests, and molecular approaches in an Iranian population. Microbial specimens were collected from 36 patients with primary endodontic infection and 14 patients with a history of root canal therapy. Advanced microbiological culture techniques were used to isolate microbiota; subsequently, biochemical tests and 16S ribosomal RNA gene sequencing were performed to identify the microorganisms. Within the total 218 cultivable isolates, Veillonella parvula (20.6%) was found to occur with the highest frequency in primary endodontic infection, followed by Porphyromonas gingivalis (14.1%), and Aggregatibacter actinomycetemcomitans (9.2%). Enterococcus faecalis (36.6%) was the most predominant microorganism in secondary endodontic infections, followed by Candida albicans, Propionibacterium acnes, and V. parvula with frequencies of 20%, 2%, and 2%, respectively. It was concluded that V. parvula and E. faecalis was most frequently found in primary and secondary endodontic infections, respectively.

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“…According to the previous studies, 9 – 11 stock solutions of TBO and MB (both items were purchased from Sigma-Aldrich) at final concentrations of 0.1 mg/mL and ICG (Santa Cruz Biotechnology Co., Ltd; Shanghai, China) stock solution at a final concentration of 1.0 mg/mL were prepared as photosensitizers in sterile 0.9% (wt/vol) NaCl. Prior to use, the photosensitizers were filtered with a sterile syringe filter of 0.22 μm pore size and were kept under dark conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Endodontic pathogens such as P. gingivalis may be exposed by a sublethal dose of PAD (sPAD; i.e., a combination of sub-minimum inhibitory concentration of photosensitizers and/or a sublethal dose of diode laser irradiation time) at the site of infection during endodontic treatment, which not only does not eradicate or inactivate the P. gingivalis but also may affect a broad range of its features and virulence factors. 7 , 8 Some studies 9 – 11 have provided evidence for the effects of sPAD with different photosensitizers and the corresponding wavelengths of light on the count and biofilm formation ability of P. gingivalis , but the sPAD effects on the expression of P. gingivalis virulence factor genes, including those that are involved in response to oxidative stress, are still missing. The aim of this study was to assess the effects of sPAD treatment in vitro on oxyR gene expression of clinical P. gingivalis isolates surviving photodynamic treatment.…”

Section: Introductionmentioning

confidence: 99%

Expression patterns of <em>oxyR</em> induced by oxidative stress from <em>Porphyromonas gingivalis</em> in response to photo-activated disinfection

Pourhajibagher¹,

Ghorbanzadeh²,

Bahador³

2018

IDR

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IntroductionPorphyromonas gingivalis, an important endodontic pathogen, may be exposed to sublethal doses of photo-activated disinfection (sPAD) during root canal therapy. Such an exposure can affect bacterial survival and virulence features. In this study, we evaluated the effect of sPAD-related oxidative stresses on the expression of oxidative stress response gene (oxyR) in P. gingivalis clinical isolates surviving in vitro photodynamic treatment.Materials and methodsTo determine the sPAD, 16 clinical P. gingivalis isolates photosensitized with toluidine blue O (TBO), methylene blue (MB), and indocyanine green (ICG) were irradiated with specific wavelength and energy density of diode laser corresponding to the photosensitizers following bacterial viability measurements. The effects of sPAD on the expression ratio of oxyR of 16 clinical P. gingivalis isolates were then assessed using quantitative real-time PCR (qRT-PCR) assay.ResultsMaximum values of sPAD against P. gingivalis were 6.25, 15.6, and 25 μg/mL at fluencies of 171.87, 15.6, and 93.75 J/cm2, respectively, for TBO-, ICG-, and MB-sPAD (P>0.05). ICG-, MB-, and TBO-sPAD could increase the oxyR gene expression of the clinical P. gingivalis isolates 12.3-, 5.6-, and 8.5-fold, respectively. ICG-sPAD increased the expression of oxyR gene in clinical isolates of P. gingivalis ~1.5- and 2-fold higher than TBO- and MB-sPAD, respectively.ConclusionOur results showed that upregulation of oxyR during sPAD may lead to better survival and increased pathogenicity of P. gingivalis isolates. Therefore, selection of appropriate photo-activated disinfection dosage should be considered for the successful treatment of endodontic infection.

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Evaluation of photo-activated disinfection effectiveness with methylene blue against Porphyromonas gingivalis involved in endodontic infection: An in vitro study

Cited by 29 publications

References 15 publications

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

Culture-dependent approaches to explore the prevalence of root canal pathogens from endodontic infections

Expression patterns of <em>oxyR</em> induced by oxidative stress from <em>Porphyromonas gingivalis</em> in response to photo-activated disinfection

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