Evaluation of Phospholipid and Fatty Acid Compositions as Chemotaxonomic Markers of Alteromonas -Like Proteobacteria

Ivanova, Elena P.; Zhukova, Natalia V.; Svetashev, Vassilii I.; Gorshkova, N. M.; Kurilenko, Valeriya V.; Frolova, Galina M.; Mikhailov, Valery V.

doi:10.1007/s002840010146

Cited by 59 publications

(36 citation statements)

References 19 publications

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“…These authors found a relatively high proportion of hydroxy fatty acids (up to 10 %), whilst in our experiments, C 12 : 0 3-OH was detected in lower amounts (up to 0?7 %). Such variation in the proportion of fatty acids was observed previously for other Proteobacteria (Huys et al, 1994;Ivanova et al, 2000) and can be explained by differing experimental conditions employed in different laboratories. In addition, a greater proportion of C 16 : 1 v9c in the fatty acid profiles of the new isolates compared to those of other type strains and a number of differences in distribution of fatty acids present in minor amounts, i.e.…”

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confidence: 67%

“…Isoprenoid quinones were extracted from lyophilized cells and analysed as described previously (Ivanova et al, 2003) according to Moule & Wilkinson (1987). Lipids were extracted as described previously (Ivanova et al, 2000). TLC revealed that phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) were the major phospholipids; the amount of PE ranged from 48?1 to 54?8 %, whilst the amount of PG ranged from 29?0 to 38?4 %.…”

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confidence: 99%

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Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan

Gorshkova

Ivanova

Sergeev

et al. 2003

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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(Rontani et al., 1999;Hedlund et al., 2001;Melcher et al., 2002). Intensive studies of Marinobacter spp. metabolites of sufficient structural specificity suggest that they may have an important role as biological markers for microbial degradation in the aquatic environment.In this study, we present the characterization of novel bacteria of the genus Marinobacter that were isolated from sediments collected in Chazhma Bay, Sea of Japan. This work was part of the taxonomic investigation of free-living marine bacteria dwelling in the Bay, sediments of which were contaminated by radionuclides (Ivanova et al., 2002). Sediment samples were collected in 2001 from a depth of 0?5 m (salinity, 32 %; temperature, 12 u C) at Chazhma Bay, Sea of Japan. Bacteria were isolated by plating 0?1 ml of a suspension of 1 g sediment in 10 ml sterilized natural sea water onto marine 2216 agar plates (Difco) or plates with medium B that contained 0?2 % (w/v) Bacto peptone (Difco), 0?2 % (w/v) casein hydrolysate (Merck), 0?2 % (w/v) Bacto yeast extract (Difco), 0?1 % (w/v) glucose,

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confidence: 67%

mentioning

confidence: 99%

Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan

Gorshkova

Ivanova

Sergeev

et al. 2003

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…The fatty acid profiles of strains S18K6 T and S18K5 clearly resemble those determined for other marine genera of the Gammaproteobacteria, e.g. Alteromonas, Pseudoalteromonas and Glaciecola (Ivanova et al, 2000).…”

supporting

confidence: 57%

Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium

Matsuyama

Hirabayashi

Kasahara

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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Two novel exopolysaccharide-producing bacteria, strains S18K6 T and S18K5, were isolated from Pacific Ocean sediment. The isolates were Gram-negative, motile, strictly aerobic chemoheterotrophic bacteria. The DNA G+C contents of strains S18K6 T and S18K5 were 44?8 and 46?3 mol%, respectively. DNA-DNA relatedness between the two strains was 70 %. Major fatty acids were hexadecanoic acid (C 16 : 0 ), hexadecenoic acid (C 16 : 1 v7c) and octadecenoic acid (C 18 : 1 v7c). 16S rRNA gene sequence, chemotaxonomic and morphological data indicated that these strains clearly belonged to the genus Glaciecola. Based on phenotypic properties and DNA-DNA hybridization data, strains S18K6 T and S18K5 are considered to represent a novel species of the genus Glaciecola, for which the name Glaciecola chathamensis sp. nov. is proposed. The type strain is S18K6 T (=JCM 13645 T =NCIMB 14146 T ).There have been many reports of micro-organisms that produce exopolysaccharides (EPSs). Some bacterial polysaccharides are produced on an industrial scale and are used as raw materials in food processing and in medical and industrial preparations. It remains possible that new polysaccharide-producing bacteria will be found in various habitats. In attempts to find new polysaccharide-producing bacteria from ocean bottom sediment we have successfully isolated numerous bacteria that produced polysaccharide in our laboratory. We describe here two polysaccharideproducing bacterial strains that were considered to belong to the genus Glaciecola. The genus Glaciecola is a member of the Gammaproteobacteria (Bowman et al., 1998). The physiological and biochemical features, chemotaxonomic characteristics and phylogeny of the two strains, designated S18K6 T and S18K5, were examined. DNA-DNA relatedness data showed that the strains should be classified as a novel species of the genus Glaciecola.Sediment samples were collected during the R/V HakuhoMaru cruise (KH-05-2) in 2005. Strains S18K6 T and S18K5 were isolated by selective enrichment from a sediment sample taken from the Pacific Ocean floor near Chatham Rise at a water depth of 4627 m (39 u 599 570 S 169 u 599 850 W). The sediment sample was inoculated in 10 ml mineral salts medium containing (per litre) 5 g peptone, 10 g glucose, 0?008 g Na 2 HPO 4 , 0?0016 g NH 4 NO 3 , 23?4 g NaCl, 0?7 g KCl, 10?6 g MgCl 2 .6H 2 O, 1?1 g CaCl 2 , 3?9 g Na 2 SO 4 , 0?2 g NaHCO 3 , 1?0 g (NH 4 ) 2 SO 4 , 0?01 g K 2 HPO 4 and 6?05 g Tris, pH 7?8. This medium was incubated at 15 u C with shaking at 150 r.p.m. After being incubated for several days, a portion of the suspension was transferred into 10 ml fresh medium and the medium was then reincubated. After three successive transfers, the suspension was plated onto solid medium to isolate pure cultures. The isolates were checked for their ability to produce EPSs by adding ethanol to the broth supernatant. Of the strains isolated, S18K6 T and S18K5, which showed good EPS production, were selected for further study. To investigate their morphological and physiological c...

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“…The fatty acid profile of strain k53 T clearly resembles those determined for other marine genera of the Gammaproteobacteria, e.g. Alteromonas, Pseudoalteromonas and Glaciecola (Ivanova et al, 2000).Bacterial DNA was prepared according to the method of Marmur (1961). The DNA obtained was digested with nuclease P1 (Yamasa Shoyu) and resulting nucleotides were separated by HPLC (Tamaoka & Komagata, 1984).…”

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confidence: 90%

Pseudoalteromonas arabiensis sp. nov., a marine polysaccharide-producing bacterium

Matsuyama

Minami

Kasahara

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A novel exopolysaccharide-producing bacterium, designated strain k53 T , was isolated from sediment from the Arabia Sea, Indian Ocean. The strain was Gram-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na + for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of C 16 : 1 v7c, C 16 : 0 and C 18 : 1 v7c. The DNA G+C content was 43 mol%. 16S rRNA gene sequence analysis suggested that strain k53 T is a member of the genus Pseudoalteromonas. Strain k53 T exhibited close phylogenetic affinity to Pseudoalteromonas lipolytica LMEB 39 T (98.0% 16S rRNA gene sequence similarity) and Pseudoalteromonas donghaensis HJ51 T (97.3 %).The DNA-DNA reassociation values between strain k53 T and P. lipolytica JCM 15903 T and P. donghaensis LMG 24469 T were 17 % and 12 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analysis based on the 16S rRNA gene sequence and DNA-DNA relatedness data, the isolate merits classification as a representative of a novel species, for which the name Pseudoalteromonas arabiensis is proposed. The type strain of this species is k53 T (5JCM 17292 T 5NCIMB 14688 T ).There have been many reports of marine micro-organisms that produce exopolysaccharides (EPSs). It remains possible that new polysaccharide-producing bacteria could be found in various habitats, and we have been trying to find them in sediment from the ocean bed (Matsuyama et al., 2006). Strain k53 T was isolated as a polysaccharide producer and was considered to be a 'Pseudoalteromonas-like' strain. The genus Alteromonas originally described by Baumann et al. (1972) for marine aerobic, Gram-negative, non-fermentative, polarly flagellated bacteria, was later divided into two genera, Alteromonas and Pseudoalteromonas, on the basis of phylogenetic analysis (Gauthier et al., 1995). At the time of writing, the genus Pseudoalteromonas comprised 38 species and two subspecies (http://www.bacterio.cict.fr/p/pseudoalteromonas. html). Members of the genus Pseudoalteromonas have been isolated from marine environments and the genus is one of the largest within the class Gammaproteobacteria. In this study, we isolated a polysaccharide-producing bacterium 'belonging to genus Pseudoalteromonas' and the phenotypic and chemotaxonomic characteristics and results of phylogenetic analysis based on the 16S rRNA gene sequence of the isolate showed that it merited classification as a novel Pseudoalteromonas species. Sediment samples were collected from the Arabia Sea at a depth of 3615 m (16 u 459 0199 N, 69 u 009 0299 E) using a multiple corer during the GEOTRACES cruise in 2009. Strain k53 T was isolated by selective enrichment. The sediment sample was inoculated in 10 ml mineral salts medium (KJ medium) containing (l 21 ) 23.4 g NaCl, 5 g MgCl 2 , 1.1 g CaCl 2 . 2H 2 O, 0.7 g KCl, 0.2 g NaHCO 3 , 6.05 g Tris, 5 g peptone, 10 g glucose, 0.01 g K 2 HPO 4 , 0.008 g Na 2 HPO 4 , 1.0 g (NH 4 ) ...

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Evaluation of Phospholipid and Fatty Acid Compositions as Chemotaxonomic Markers of Alteromonas -Like Proteobacteria

Cited by 59 publications

References 19 publications

Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan

Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan

Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium

Pseudoalteromonas arabiensis sp. nov., a marine polysaccharide-producing bacterium

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