Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan

Abstract

(Rontani et al., 1999;Hedlund et al., 2001;Melcher et al., 2002). Intensive studies of Marinobacter spp. metabolites of sufficient structural specificity suggest that they may have an important role as biological markers for microbial degradation in the aquatic environment.In this study, we present the characterization of novel bacteria of the genus Marinobacter that were isolated from sediments collected in Chazhma Bay, Sea of Japan. This work was part of the taxonomic investigation of free-living marine bacteria dwelling in the Bay, sediments of which were contaminated by radionuclides (Ivanova et al., 2002). Sediment samples were collected in 2001 from a depth of 0?5 m (salinity, 32 %; temperature, 12 u C) at Chazhma Bay, Sea of Japan. Bacteria were isolated by plating 0?1 ml of a suspension of 1 g sediment in 10 ml sterilized natural sea water onto marine 2216 agar plates (Difco) or plates with medium B that contained 0?2 % (w/v) Bacto peptone (Difco), 0?2 % (w/v) casein hydrolysate (Merck), 0?2 % (w/v) Bacto yeast extract (Difco), 0?1 % (w/v) glucose,

Marinobacter goseongensis sp. nov., from seawater

“…Since this genus was proposed in 1992, more than 15 species of Marinobacter have been isolated from various environments, including: seawater (Antunes et al, 2007;Gauthier et al, 1992;Shivaji et al, 2005;Yoon et al, 2003Yoon et al, , 2004, oilproducing well (Huu et al, 1999), marine sediment (Gorshkova et al, 2003;Romanenko et al, 2005), saline soil Martin et al, 2003), coastal hot spring (Shieh et al, 2003), wastewater (Liebgott et al, 2006), laboratory cultures of dinoflagellates (Green et al, 2006) and sea sand (Kim et al, 2006). In this paper, the taxonomic position of strain En6 T was established by means of phenotypic, genetic and chemotaxonomic analyses.…”

Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands

“…Cells of strain CK 47 T were Gram-negative, motile, rod-shaped and non-spore-forming. CK 47 T had Q9 as the major respiratory ubiquinone, C 16 : 0 (21?7 %), C 17 : 0 (21?3 %), C 18 : 0 (5?7 %), C 18 : 1 v7c (9?0 %) and C 18 : 1 v9c (31?4 %) as the predominant fatty acids (Supplementary Table S2) and a G+C content of the DNA of 58?0 mol%, similar to species of the genus Marinobacter (Nguyen et al, 1999;Gorshkova et al, 2003;Yoon et al, 2003Yoon et al, , 2004. The G+C content of the DNA in the genus Marinobacter varies from 53 to 58 mol%.…”

“…Species of the genus, including Marinobacter hydrocarbonoclasticus (Gauthier et al, 1992) et al, 2004), possess a large number of saturated and unsaturated fatty acids and ubiquinone-9 (Q9) as the major respiratory pigment (Gorshkova et al, 2003;Yoon et al, 2003Yoon et al, , 2004. Based on a polyphasic taxonomic approach, a Gramnegative motile bacterium is identified here as representing a novel species of the genus Marinobacter, for which the name Marinobacter maritimus sp.…”

“…Species of the genus, including Marinobacter hydrocarbonoclasticus (Gauthier et al, 1992), Marinobacter aquaeolei (Nguyen et al, 1999), Marinobacter excellens (Gorshkova et al, 2003), Marinobacter lipolyticus (Martin et al, 2003), Marinobacter litoralis (Yoon et al, 2003), Marinobacter flavimaris and Marinobacter daepoensis (Yoon et al, 2004), possess a large number of saturated and unsaturated fatty acids and ubiquinone-9 (Q9) as the major respiratory pigment (Gorshkova et al, 2003;Yoon et al, 2003Yoon et al, , 2004. Based on a polyphasic taxonomic approach, a Gramnegative motile bacterium is identified here as representing a novel species of the genus Marinobacter, for which the name Marinobacter maritimus sp.…”

Marinobacter adhaerens sp. nov., isolated from marine aggregates formed with the diatom Thalassiosira weissflogii

“…These species are tolerant to various conditions as they were isolated from diverse locations, i.e. from sediment (Gorshkova et al, 2003), the water column (Yoon et al, 2004), coastal (Roh et al, 2008) and deep-sea waters (Takai et al, 2005), the Antarctic (Montes et al, 2008) and the Red Sea (Antunes et al, 2007). Furthermore, representatives of this genus have been isolated from oil-contaminated areas (Huu et al, 1999), hot springs (Shieh et al, 2003) and saline environments (Martín et al, 2003).…”