Evaluation of Pegylated Nanoniosomal Cisplatin on Rat C6 in Vitro.

Abstract

Cisplatin is one of the most useful drugs in chemotherapy, which have several therapeutic properties. Nevertheless, this drug carries some side effects. In order to reduce such effects, nanotechnology has been a great help. In this study pegylated nanoniosomal Cisplatin was prepared through reverse phase evaporation technique. Certain ratios of span 60, cholesterol and polyethylene glycol (3000 Da) were synthesized to prepare pegylated nanoniosomal Cisplatin. The mean diameter, size distribution and zeta potential of pegylated nanoniosomal containing drug and without drug was measured 205.5±4.60 nm, 0.253±0.0036 and-21.4 mv; 251.1±3.93 nm, 0.058±0.0075 and-22.6 mv, respectively using Zetasizer. Encapsulation and drug loading efficiency of pegylated nanoniosomal Cisplatin was determined by spectrophotometry method that were 48.2±2.05% and 4.38±0.28%, respectively. The percent of drug released from niosomes was performed by dialysis for 48 hours. The amount of released drug from niosomes indicated that 82.73±1.36% of drug released into the PBS. Also, this study investigated the cytotoxicity effect of pegylated nanoniosomal Cisplatin using MTT assay. The results showed that IC50 of the pegylated nanoniosomal Cisplatin formulation is less than free drug on C6 cell line.