Evaluation of pathogencity and race classification of &amp;lt;i&amp;gt;Xanthomonas oryzae&amp;lt;/i&amp;gt; pv. &amp;lt;i&amp;gt;oryzae&amp;lt;/i&amp;gt; in guilan province-Iran

Khoshkdaman, Maryam; Ebadi, A.; Kahrizi, Danial

doi:10.4236/as.2012.34066

Cited by 10 publications

(6 citation statements)

References 13 publications

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“…In this study, we establish the level of resistance to the bacterial blight pathogen conferred by a single gene individually and multiple gene combinations. Previous results on the basis of host-pathogen interaction showed that there were four bacterial leaf blight races in Iran [28]. These races were tested on 12 international differentials of near-isogenic lines and 14 varieties of pyramided lines and 8 local and improved varieties to support a gene deployment approach to managing the disease using resistant cultivars.…”

Section: Discussionmentioning

confidence: 99%

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Preliminary evaluation of resistance genes in rice against bacterial leaf blight in Guilan Province—Iran

Khoshkdaman¹,

Ebadi²,

Majidi-Shilsar³

et al. 2014

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The reactions of rice bacterial leaf blight races were identified in Guilan province-Iran on 12 near-isogenic lines and 14 pyramiding lines from International Network for Genetic Evaluation of rice (INGER) and 8 local and improved Iranian varieties were evaluated under natural photoperiod condition in the field. Inoculation was done at panicle initiation by clipping the sterilized scissors in the bacterial suspension to booting stage. Scoring of inoculated plants was made 21 days after inoculation. Infection levels of pyramiding lines containing two to five resistance genes, expect, IRBB53 and IRBB61 with respectively resistance gene combination, Xa5 + Xa13 and Xa4 + Xa5 + Xa7, were not so clear. Among near-isogenic lines IRBB1, IRBB2, IRBB4 and IRBB10 carrying resistance gene Xa1, Xa2, Xa4 and Xa10 were susceptible; IRBB8, IRBB11, IRBB3, IRBB5 and IRBB13 were moderately susceptible; (having resistance gene Xa8, Xa11, Xa3, Xa5 and Xa13) IRBB14, IRBB21 and IRBB7 with respectively resistance gene Xa14, Xa21 and Xa7 were moderately resistance to bacterial blight. Furthermore, most of the time gene combinations support the strategy of pyramiding appropriate resistance gene. Local varieties were more susceptible than improved varieties to leaf blight disease. Among local varieties, Tarom was the most susceptible. And also, there were no significant differences among improved varieties and all of them were moderately resistance.

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Section: Discussionmentioning

confidence: 99%

“…In Guilan province, previous results on BLB showed that there were four races of X. oryzae pv. oryzae [28]. The purpose of this study is to identify resistance sources for controlling the rice bacterial blight in Guilan-Iran.…”

Section: Introductionmentioning

confidence: 99%

Preliminary evaluation of resistance genes in rice against bacterial leaf blight in Guilan Province—Iran

Khoshkdaman¹,

Ebadi²,

Majidi-Shilsar³

et al. 2014

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“…Galur isogenik bermanfaat dalam monitoring keparahan serangan dan distribusi ras Xoo (Ogawa et al, 1988). Di samping itu, galur-galur isogenik juga dapat digunakan sebagai standar diferensial untuk mengetahui perbedaan respons patogenisitas atau virulensi suatu ras (Hayashi dan Fukuta, 2007;Khoshkdaman et al, 2012;Loan et al, 2006;Noh et al, 2016;Tsunematsu et al, 2000) dan untuk evaluasi ketahanan galur hasil persilangan (silang balik) dalam rangka perakitan varietas tahan HDB (Webb et al, 2010).…”

Section: Pendahuluanunclassified

Identifikasi Gen Ketahanan terhadap (Xanthomonasoryzae pv. Oryzae), penyebab Penyakit Hawar Daun Bakteri padaPlasma Nutfah Padi Lokal Sulawesi Selatan

Yuriyah¹,

Nurani

Utami³

et al. 2018

J. AgroBiogen

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One of the limiting factors on rice production, especially in South Sulawesi, is the bacterial leaf blight (BLB) disease infection caused by Xanthomonas oryzae pv. oryzae (Xoo). The use of resistant variety is considered as the most effective method to control Xoo. Local cultivar could serve as resistance source for Xoo resistance. The objectives of this research were to evaluate and identify markers for Xoo resistance in rice local cultivars from South Sulawesi based on comparison with the resistance response of the differential varieties and association analysis between resistance phenotype and genotype of molecular markers. Three Xoo races (race III, IV, and VIII) were tested on IRBB differential lines and local cultivars. The genotype analysis was done by using the molecular marker linked to Xoo resistance. Meanwhile, the association analysis was done by combination analysis (U-joint) using generalized linear model (GLM). The resistance test result showed that single gene isogenic lines (IRBB 5, IRBB 7, and IRBB 21), multiple genes isogenic lines (IRBB 50, IRBB 52, IRBB 53, IRBB 54, IRBB 56, IRBB 58, IRBB 64, and IRBB 66), and Ase Andele accession were resistant to the three Xoo races. Association test obtained one significant marker that associated with the resistance to race III (marker Xa26-STS2), four markers significantly associated with the resistance to race IV (Xa1-STS15, Xa4-STS44, xa13-STS51, and Xa21-STS6), and two markers significantly associated with the resistance to race VIII (Xa7-STS57 and RM 20589). Keywords:Resistance gene, bacterial leaf blight, germplasm. ABSTRAKSalah satu faktor pembatas produksi padi, di Sulawesi Selatan khususnya, adalah serangan penyakit hawar daun bakteri (HDB) yang disebabkan oleh Xanthomonas oryzae pv. oryzae (Xoo). Upaya yang dipandang efektif untuk mengendalikan penyakit HDB adalah penanaman varietas tahan. Perakitan varietas padi dengan menggunakan gen-gen tahan dari berbagai kultivar berpeluang menghasilkan varietas tahan HDB. Kultivar lokal berperan sebagai salah satu sumber keragaman genetik untuk beberapa sifat ketahanan penyakit. Penelitian ini bertujuan mengevaluasi dan mengidentifikasi marka penanda gen ketahanan penyakit hawar daun bakteri pada padi lokal Sulawesi Selatan berdasarkan sistem standar diferensial dan analisis molekuler. Analisis uji ketahanan fenotipe dilakukan menggunakan tiga ras HDB (ras III, IV, dan VIII) dan galur diferensial (galur IRBB). Analisis genotipe dilakukan menggunakan marka molekuler terkait ketahanan terhadap HDB yang ditampilkan sebagai dendrogram keragaman. Analisis asosiasi dilakukan dengan analisis gabungan (U-joint) menggunakan generalized linear model (GLM). Hasil pengujian ketahanan menunjukkan galur isogenik dengan gen tunggal (IRBB 5, IRBB 7, dan IRBB 21), galur isogenik dengan multipel gen (IRBB 50, IRBB 52, IRBB 53, IRBB 54, IRBB 56, IRBB 58, IRBB 64, dan IRBB 66), dan aksesi Ase Andele bersifat tahan terhadap ketiga ras uji. Dari uji asosiasi, diperoleh satu marka signifikan yang berasosiasi dengan k...

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“…Near isogenic lines (NILs) with specific resistance genes have been identified and among them, lines with a single resistance gene are widely used to characterize virulence and differentiate pathogenic races of Xoo. Classification of the pathogenic race of Xoo is based on the interaction between its avr gene and rice Xa gene [23,[26][27][28]. In this study, we used IRBB4 line carrying Xa4 gene and the susceptible genotype FKR14.…”

Section: Strain Virulencementioning

confidence: 99%

“…The phenotypic reactions of the bacteria strains and rice line IRBB4 were similar to the reaction observed on FKR14 except with strain Mai1. IRBB4 line shows susceptibility to Xoo strains from Iran [28], while in China; it is susceptible to 50% of Chinese Xoo strains [29]. In earlier 1970, the Xoo populations were dominated by those strains that could not infect rice lines with Xa4 gene [30], while the newly developed rice lines with Xa4 gene are nowadays susceptible to several strains [31].…”

Section: Strain Virulencementioning

confidence: 99%

Biochemical Approach for Virulence Factors’ Identification in Xanthomonas Oryzae Pv. Oryzae

C¹,

Karlovský²,

Wydra³

2014

J Plant Pathol Microbiol

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Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) leads to a substantial yield reduction of up to 50% in most rice-growing regions. Host plant resistance is an effective control method, and more than 30 resistance genes have been identified in rice genotypes. To understand the interaction of the pathogen in a susceptible reaction of the host plant, Xoo culture filtrate and treated culture filtrates were used to treat two rice genotypes using four strains Mai1, PXO88, Dak1 and Dak16. The study revealed that Xoo culture filtrate, heated culture filtrate and proteinase K treated culture filtrate induced typical bacterial blight symptoms on rice genotypes IRBB4 and FKR14 with a maximum lesion length of about 23.1 cm for culture filtrate. Heated culture filtrate phytotoxicity effects on both rice genotypes was with highest lesion length of about 6.9 cm, while 13.4 cm was the maximum length induced by a proteinase K treated fraction. After ethyl acetate treatment of the culture filtrate, a considerable reduction of the phytotoxicity was observed. Therefore we suggest that a low molecular-weight toxin may be present in the ethyl acetate extract should not play a major role in Xoo virulence and speculate that EPS, Xylanase, polygalacturonase, proteinaceouse contribute to Xoo virulence.

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Evaluation of pathogencity and race classification of <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> in guilan province-Iran

Cited by 10 publications

References 13 publications

Preliminary evaluation of resistance genes in rice against bacterial leaf blight in Guilan Province—Iran

Preliminary evaluation of resistance genes in rice against bacterial leaf blight in Guilan Province—Iran

Identifikasi Gen Ketahanan terhadap (Xanthomonasoryzae pv. Oryzae), penyebab Penyakit Hawar Daun Bakteri padaPlasma Nutfah Padi Lokal Sulawesi Selatan

Biochemical Approach for Virulence Factors’ Identification in Xanthomonas Oryzae Pv. Oryzae

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